POPULATION DYNAMICS OF AN ENDOGENOUS MEIOTIC DRIVE SYSTEM IN AEDES AEGYPTI IN TRINIDAD

2006 ◽  
Vol 75 (1) ◽  
pp. 70-77 ◽  
Author(s):  
SUNG-JAE CHA ◽  
DAVE D. CHADEE ◽  
DAVID W. SEVERSON
Keyword(s):  
Population Dynamics ◽  
Aedes Aegypti ◽  
Meiotic Drive ◽  
Drive System

scholarly journals Genome-Wide Transcriptome Profiling Reveals Genes Associated with Meiotic Drive System of Aedes aegypti

Insects ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 25 ◽  
Author(s):  
Dongyoung Shin ◽  
Susanta Behura ◽  
David Severson
Keyword(s):  
Aedes Aegypti ◽  
Meiotic Drive ◽  
Transcriptome Profiling ◽  
Protein Translation ◽  
Drive System ◽  
Differentially Expressed ◽  
Mosquito Vector ◽  
Whole Genome ◽  
Bioinformatics Analyses ◽  
Whole Genome Microarray

Aedes aegypti is an important mosquito vector of several arboviruses, including dengue, yellow fever, Zika, and Chikungunya, which cause significant human morbidity and mortality globally. In certain populations of this mosquito, a native meiotic drive system causes abnormal spermatogenesis that results in highly male-biased progenies from some matings. Although the basic genetics and cytogenetics of the drive mechanism were elucidated, very little is known on a transcriptome level about how the meiotic drive phenotype is expressed in individual males. To address this question, we conducted a whole-genome microarray expression study of testes from a meiotic-drive-carrying strain (T37) in comparison with testes from a non-drive-carrying strain (RED). Based on bioinformatics analyses of the microarray data, we identified 209 genes associated with the meiotic drive phenotype that were significantly differentially expressed between the two strains. K-means cluster analysis revealed nine clusters, in which genes upregulated in T37 testes were assigned to five clusters and genes downregulated in T37 testes were assigned to four clusters. Our data further revealed that genes related to protein translation, phosphorylation, and binding, as well as to G-protein-coupled receptor (GPCR) and peptidase activities, are differentially upregulated in testes from males with the meiotic drive genotype. Based on pathway analysis of these differentially expressed genes, it was observed that the glycosylphosphatidylinositol (GPI)-anchor biosynthesis pathway may play a role in the meiotic drive system. Overall, this investigation enhances our understanding of whole-genome gene expression associated with the meiotic drive system in Ae. aegypti.

scholarly journals A CRISPR endonuclease gene drive reveals two distinct mechanisms of inheritance bias

2020 ◽  
Author(s):  
Sebald A.N. Verkuijl ◽  
Estela González ◽  
Joshua Xin De Ang ◽  
Ming Li ◽  
Nikolay P Kandul ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Homing Endonuclease ◽  
Meiotic Drive ◽  
Drive System ◽  
Transgene Inheritance ◽  
Gene Drive ◽  
Tight Linkage ◽  
Multiple Species ◽  
Gene Drives ◽  
Endonuclease Gene

RNA guided CRISPR gene drives have shown the capability of biasing transgene inheritance in multiple species. Among these, homing endonuclease drives are the most developed. In this study, we report the functioning of sds3, bgcn, and nup50 expressed Cas9 in an Aedes aegypti homing split drive system targeting the white gene. We report their inheritance biasing capability, propensity for maternal deposition, and zygotic/somatic expression. Additionally, by making use of the tight linkage of white to the sex-determining locus, we were able to elucidate mechanisms of inheritance bias. We find inheritance bias through homing in double heterozygous males, but find that a previous report of the same drive occurred through meiotic drive. We propose that other previously reported 'homing' design gene drives may in fact bias their inheritance through other mechanisms with important implications for gene drive design.

scholarly journals Reinvestigation of an Endogenous Meiotic Drive System in the Mosquito, Aedes aegypti (Diptera: Culicidae)

2004 ◽  
Vol 41 (6) ◽  
pp. 1027-1033 ◽  
Author(s):  
Akio Mori ◽  
Dave D. Chadee ◽  
Douglas H. Graham ◽  
David W. Severson
Keyword(s):  
Aedes Aegypti ◽  
Meiotic Drive ◽  
Drive System

A model for the population dynamics of the vector Aedes aegypti (Diptera: Culicidae) with control

2018 ◽  
Vol 12 (7) ◽  
pp. 311-325 ◽  
Author(s):  
Julian Alejandro Olarte Garcia ◽  
Anibal Munoz Loaiza ◽  
Carlos Alberto Abello Munoz
Keyword(s):  
Population Dynamics ◽  
Aedes Aegypti

scholarly journals Water Level Flux in Household Containers in Vietnam - A Key Determinant of Aedes aegypti Population Dynamics

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e39067 ◽  
Author(s):  
Jason A. L. Jeffery ◽  
Archie C. A. Clements ◽  
Yen Thi Nguyen ◽  
Le Hoang Nguyen ◽  
Son Hai Tran ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Aedes Aegypti ◽  
Water Level ◽  
Aegypti Population

scholarly journals Understanding Uncertainties in Model-Based Predictions of Aedes aegypti Population Dynamics

2010 ◽  
Vol 4 (9) ◽  
pp. e830 ◽  
Author(s):  
Chonggang Xu ◽  
Mathieu Legros ◽  
Fred Gould ◽  
Alun L. Lloyd
Keyword(s):  
Population Dynamics ◽  
Aedes Aegypti ◽  
Model Based ◽  
Aegypti Population
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