Protective effect of Qingxin Kaiqiao Fang containing cerebrospinal fluid on PC12 cell injury induced by Aβ25-35

2012 ◽  
Author(s):  
YE Guang-hua
Keyword(s):  
Cerebrospinal Fluid ◽  
Protective Effect ◽  
Pc12 Cell ◽  
Cell Injury

scholarly journals RNA-Seq Based Transcriptome Analysis of Ethanol Extract of Saffron Protective Effect Against Corticosterone-Induced PC12 Cell Injury

2021 ◽  
Author(s):  
Xi Chen ◽  
Ting Yang ◽  
Cong' en Zhang ◽  
Zhijie Ma
Keyword(s):  
Protective Effect ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Cell Injury ◽  
Ethanol Extract ◽  
Mapk Signaling ◽  
Cell Counting ◽  
Rna Seq ◽  
Traditional Chinese Herbal Medicine ◽  
Water Test

Abstract Background Saffron is a traditional Chinese herbal medicine, which is typically used in clinical to regulate anxiety, tension, and other depression-related conditions. The study aimed to explore the neuroprotective effect of ethanol extract of saffron (EES) on corticosterone (CORT)-induced injury in PC12 cells and further explored its potential mechanism. Methods The authenticity of saffron and the active components of EES were identified by a water test and ultra-performance liquid chromatography-time of flight mass spectrometry system. The screening of cytotoxicity for PC12 cells was incubated with EES in different concentrations for 24 h, and the protective efficacy of EES on CORT (500 µM) induced PC12 cell injury, cell viability was assessed by Cell Counting Kit-8 (CCK-8) assay. The DEGs of EES-protected PC12 cells were analyzed using the RNA-seq method, and the results were analyzed for GO and KEGG enrichment. The results of RNA-seq were verified by qPCR analysis. Results The saffron was initially identified as authentic in the water test and 10 compounds were identified by UPLC-MS. The results of CCK-8 demonstrated that EES at concentrations above 640 µg/mL exerted a certain cytotoxic effect, and PC12 cells pretreated with EES (20, 40, and 80 µg/mL) significantly reversed the 500 µM CORT-induced cell death. RNA-seq analysis showed that EES regulated 246 differential genes, which were mainly enriched in the MAPK signaling pathway. Dusp5, Dusp6, Gadd45b, Gadd45G, and Pdgfc were further validated by qPCR. Experimental data showed that the results of qPCR were consistent with RNA-seq. Conclusions These findings provide an innovative understanding of the molecular mechanism of the protective effect of EES on PC12 cells at the molecular transcription level, and the above molecules may be potential novel targets for antidepressant treatment.

scholarly journals Protective effect of HAMI 3379 against high glucose-induced PC12 cell injury

2015 ◽  
Vol 6 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Zhao Chunzhen ◽  
Wang Lin ◽  
Li Wanzhong
Keyword(s):  
Protective Effect ◽  
Pc12 Cell ◽  
High Glucose ◽  
Cell Injury

scholarly journals Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

2011 ◽  
Vol 35 (5) ◽  
pp. 469 ◽  
Author(s):  
Eun-Mi Lee ◽  
Young-Eun Lee ◽  
Esder Lee ◽  
Gyeong Ryul Ryu ◽  
Seung-Hyun Ko ◽  
...  
Keyword(s):  
Protective Effect ◽  
Heme Oxygenase ◽  
High Glucose ◽  
Cell Injury ◽  
Heme Oxygenase 1 ◽  
Pancreatic Β Cell ◽  
Β Cell

Protective Effect of Paeoniflorin on Aβ25–35-Induced SH-SY5Y Cell Injury by Preventing Mitochondrial Dysfunction

2013 ◽  
Vol 34 (2) ◽  
pp. 227-234 ◽  
Author(s):  
Ke Wang ◽  
Ling Zhu ◽  
Xue Zhu ◽  
Kai Zhang ◽  
Biao Huang ◽  
...  
Keyword(s):  
Protective Effect ◽  
Mitochondrial Dysfunction ◽  
Cell Injury

Low Frequency of Adenylate Kinase Release into Cerebrospinal Fluid during Balanced, Normotensive Anaesthesia and a Non-Orthognathic Surgical Procedure

1997 ◽  
Vol 25 (2) ◽  
pp. 92-97 ◽  
Author(s):  
M Enlund ◽  
O Mentell ◽  
L Edmark ◽  
G Ronquist
Keyword(s):  
Cerebrospinal Fluid ◽  
Adenylate Kinase ◽  
Orthognathic Surgery ◽  
Cell Injury ◽  
Low Frequency ◽  
Brain Cell ◽  
Contributing Factors ◽  
Oscillating Saw ◽  
The Difference ◽  
Increased Activity

Activity of strictly intracellular enzymes in the cerebrospinal fluid (CSF) may indicate leakage from dysfunctional brain cells. Increased activity of adenylate kinase (AK) in the CSF is indicative of brain cell injury arising from several sources, among them orthognathic surgery. The mechanism in the latter case is obscure, but the use of an oscillating saw which generates vibrations, and the site of surgery close to the brain may be contributing factors. Anaesthesia may also play a role. In the present study, CSF-AK activity was measured after hysterectomy and was compared with activity after orthognathic surgery in two other studies. Four of 19 patients (21%) in the present study expressed pathological activity, compared with 34 of 47 (72%) orthognathic patients in the two other studies. No firm conclusion may be drawn from historical comparisons, and the difference in activity seen between the two types of surgery might not necessarily be the result of surgical factors. Until this is investigated further, however, we conclude that there may be a difference in postoperative CSF-AK activity between orthognathic and lower abdominal surgery.

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