Determination of chlorogenic acid, caffeic acid and linarin in Flos Chrysanthemi Indici from different places by RP-HPLC

2010 ◽  
Vol 35 (9) ◽  
Author(s):  
GUO Qiaosheng
Keyword(s):  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Rp Hplc

scholarly journals Anti-diabetes Constituents in Leaves of Smallanthus sonchifolius

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Xiang Zheng ◽  
He Fan ◽  
Kang Ting-Guo ◽  
Dou De-Qiang ◽  
Gai Kuo ◽  
...  
Keyword(s):  
Quality Control ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Analytical Method ◽  
Inhibitory Effect ◽  
Hplc Method ◽  
Smallanthus Sonchifolius ◽  
Rp Hplc

The inhibitory effect of smallanthaditerpenic acids A, B, C and D previously isolated from leaves of Smallanthus sonchifolius (yacon) on α-glucosidase were examined and their IC50 were determined to be 0.48 mg/mL, 0.59 mg/mL, 1.00 mg/mL, and 1.17 mg/mL respectively. In addition, a rapid, reliable RP-HPLC method for the analysis of chlorogenic acid, caffeic acid, and smallanthaditerpenic acids A and C in yacon leaves was established, and the variation in their contents in leaves from plants cultivated in different places and collected at different times of the year were compared. The established analytical method for determining smallanthaditerpenic acids A and C, chlorogenic acid and caffeic acid presented good results and could be used as a method for the quality control of S. sonchifolius leaves.

scholarly journals Spectrophotometric and Smartphone-Assisted Determination of Phenolic Compounds Using Crude Eggplant Extract

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4407 ◽  
Author(s):  
M.A. Morosanova ◽  
A.S. Bashkatova ◽  
E.I. Morosanova
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Polyphenol Oxidase ◽  
Oxidase Activity ◽  
Biological Fluids ◽  
Low Cost ◽  
Analytical Signal ◽  
Polyphenol Oxidase Activity

In order to develop a simple, reliable and low cost enzymatic method for the determination of phenolic compounds we studied polyphenol oxidase activity of crude eggplant (S. melongena) extract using 13 phenolic compounds. Catechol, caffeic and chlorogenic acids, and L-DOPA have been rapidly oxidized with the formation of colored products. Monophenolic compounds have been oxidized at a much slower speed. Ferulic acid, quercetin, rutin, and dihydroquercetin have been found to inhibit polyphenol oxidase activity of crude eggplant extract. The influence of pH, temperature, crude eggplant extract amount, and 3-methyl-2-benzothiazolinone hydrazone (MBTH) concentration on the oxidation of catechol, caffeic acid, chlorogenic acid, and L-DOPA has been investigated spectrophotometrically. Michaelis constants values decrease by a factor of 2 to 3 in the presence of MBTH. Spectrophotometric (cuvette and microplate variants) and smartphone-assisted procedures for phenolic compounds determination have been proposed. Average saturation values (HSV color model) of the images of the microplate wells have been chosen as the analytical signal for smartphone-assisted procedure. LOD values for catechol, caffeic acid, chlorogenic acid, and L-DOPA equaled 5.1, 6.3, 5.8 and 30.0 µM (cuvette procedure), 12.2, 13.2, 13.2 and 80.4 µM (microplate procedure), and 23.5, 26.4, 20.8 and 120.6 µM (smartphone procedure). All the variants have been successfully applied for fast (4-5 min) and simple TPC determination in plant derived products and L-DOPA determination in model biological fluids. The values found with smartphone procedure are in good agreement with both spectrophotometric procedures values and reference values. Using crude eggplant extract- mediated reactions combined with smartphone camera detection has allowed creating low-cost, reliable and environmentally friendly analytical method for the determination of phenolic compounds.

scholarly journals RP-HPLC and HPTLC Methods for Analysis of Selected Herbs Used as Complexion Promoters in Ayurveda and Unani Systems of Medicine

2020 ◽  
Vol 103 (3) ◽  
pp. 692-698
Author(s):  
Akanksha Sharma ◽  
Chandra Kant Katiyar ◽  
Subhadip Banerjee ◽  
Joydeb Chanda ◽  
Amit Kar ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Quality Evaluation ◽  
Retardation Factor ◽  
Herbal Products ◽  
Hemidesmus Indicus ◽  
Inula Racemosa ◽  
Rp Hplc

Abstract Background: In Ayurvedic and Unani texts, many herbs and formulations are mentioned as complexion promoters having skin brightening and whitening effects that downregulate melanin synthesis. However, with the assistance of chromatographic techniques, further validation of scientific standardization is required. Objective: To validate individual herbs and formulations in the ancient literature, some scientific aspects are to be focused on, including standardization of herbs or herbal products consisting of the active compounds. Methods: Out of many herbs having complexion promoting properties, three herbs (Myristica fragrans, Hemidesmus indicus, and Inula racemosa) were selected for the study. In the present study, validated reverse phase (RP)-HPLC and high-performance TLC (HPTLC) methods were developed for standardization of the herbs. Results: It was observed that the quercetin present in M. fragrans was 0.62% (w/w) with a retention time (Rt) of 6.23 min, the ferulic acid present in H. indicus was 1.39% (w/w) with an Rt of 8.083 min, and the chlorogenic acid present in I. racemosa was 1.03% (w/w) with an Rt of 3.19 min. The HPTLC method showed 1.23% (w/w) of quercetin present in M. fragrans with a retardation factor (Rf) of 0.48, ferulic acid in H. indicus was 1.52% (w/w) with an Rf of 0.44, and chlorogenic acid was 1.09% (w/w) in I. racemosa with an Rf of 0.52. Conclusions: This specific and precise validated method can be useful for the quality evaluation and quantitative determination of the constituents in complexion-promoting herbs from Ayurveda and Unani systems of medicine. Highlights: The exploration of complexion promoters from Ayurveda and Unani; quality evaluation of complexion promoters herbs from Ayurveda and Unani; HPTLC and RP-HPLC analysis of herbal extracts used and their validation.

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