scholarly journals Tissue specific role of calcium sensing receptor

2014 ◽  
Author(s):  
P Sarkar ◽  
S Kumar
Keyword(s):  
Specific Role ◽  
Calcium Sensing Receptor ◽  
Tissue Specific ◽  
Calcium Sensing

Expression and Role of the Calcium-Sensing Receptor in the Blood Vessel Wall

2009 ◽  
Vol 10 (3) ◽  
pp. 282-288 ◽  
Author(s):  
Guerman Molostvov ◽  
Rosemary Bland ◽  
Daniel Zehnder
Keyword(s):  
Blood Vessel ◽  
Vessel Wall ◽  
Blood Vessel Wall ◽  
Calcium Sensing Receptor ◽  
Calcium Sensing

scholarly journals The role of the calcium-sensing receptor in gastrointestinal inflammation

2016 ◽  
Vol 49 ◽  
pp. 44-51 ◽  
Author(s):  
Jennifer L. Owen ◽  
Sam X. Cheng ◽  
Yong Ge ◽  
Bikash Sahay ◽  
Mansour Mohamadzadeh
Keyword(s):  
Calcium Sensing Receptor ◽  
Calcium Sensing ◽  
Gastrointestinal Inflammation

Evidence for a functional calcium-sensing receptor that modulates myogenic tone in rat subcutaneous small arteries

2005 ◽  
Vol 288 (4) ◽  
pp. H1756-H1762 ◽  
Author(s):  
Jacqueline Ohanian ◽  
Kelly M. Gatfield ◽  
Donald T. Ward ◽  
Vasken Ohanian
Keyword(s):  
Vascular Tissue ◽  
Immunoblot Analysis ◽  
Negative Regulator ◽  
Intraluminal Pressure ◽  
Myogenic Tone ◽  
Internal Diameter ◽  
Calcium Sensing Receptor ◽  
Small Arteries ◽  
Calcium Sensing

Myogenic tone of small arteries is dependent on the presence of extracellular calcium ([Formula: see text]), and, recently, a receptor that senses changes in Ca2+, the calcium-sensing receptor (CaR), has been detected in vascular tissue. We investigated whether the CaR is involved in the regulation of myogenic tone in rat subcutaneous small arteries. Immunoblot analysis using a monoclonal antibody against the CaR demonstrated its presence in rat subcutaneous arteries. To determine whether the CaR was functionally active, segments of artery (<250 μm internal diameter) mounted in a pressure myograph with an intraluminal pressure of 70 mmHg were studied after the development of myogenic tone. Increasing [Formula: see text] concentration ([Ca2+]o) cumulatively from 0.5 to 10 mM induced an initial constriction (0.5–2 mM) followed by dilation (42 ± 5% loss of tone). The dose-dependent dilation was mimicked by other known CaR agonists including magnesium (1–10 mM) and the aminoglycosides neomycin (0.003–10 mM) and kanamycin (0.003–3 mM). PKC activation with the phorbol ester phorbol-12,13-dibutyrate (20nM) inhibited the dilation induced by high [Ca2+]o or neomycin, whereas inhibition of PKC with GF109203X (10 μM) increased the responses to [Formula: see text] or neomycin, consistent with the role of PKC as a negative regulator of the CaR. We conclude that rat subcutaneous arteries express a functionally active CaR that may be involved in the modulation of myogenic tone and hence the regulation of peripheral vascular resistance.

Role of calcium-sensing receptor in cadmium-induced apoptosis of rat primary osteoblasts in vitro

2020 ◽  
Vol 67 ◽  
pp. 104923 ◽  
Author(s):  
Di Ran ◽  
Wei Liu ◽  
Yonggang Ma ◽  
Jiaming Zheng ◽  
Dedong Wang ◽  
...  
Keyword(s):  
Calcium Sensing Receptor ◽  
Calcium Sensing ◽  
Primary Osteoblasts ◽  
Induced Apoptosis

Expression of extracellular calcium-sensing receptor in human osteoblastic MG-63 cell line

2001 ◽  
Vol 280 (2) ◽  
pp. C382-C393 ◽  
Author(s):  
Toru Yamaguchi ◽  
Naibedya Chattopadhyay ◽  
Olga Kifor ◽  
Chianping Ye ◽  
Peter M. Vassilev ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Northern Blot Analysis ◽  
Pcr Primers ◽  
Extracellular Calcium ◽  
Calcium Sensing Receptor ◽  
Patch Clamp Technique ◽  
Calcium Sensing ◽  
Specific Affinity

We have previously shown the expression of the extracellular calcium (Cao 2+)-sensing receptor (CaR) in osteoblast-like cell lines, and others have documented its expression in sections of murine, bovine, and rat bone. The existence of the CaR in osteoblasts remains controversial, however, since some studies have failed to document its expression in the same osteoblast-like cell lines. The goals of the present study were twofold. 1) We sought to determine whether the CaR is expressed in the human osteoblast-like cell line, MG-63, which has recently been reported by others not to express this receptor. 2) We investigated whether the CaR, if present in MG-63 cells, is functionally active, since most previous studies have not proven the role of the CaR in mediating known actions of Cao 2+ on osteoblast-like cells. We used immunocytochemistry and Western blotting with the specific, affinity-purified anti-CaR antiserum 4637 as well as Northern blot analysis and RT-PCR using a riboprobe and PCR primers specific for the human CaR, respectively, to show readily detectable CaR protein and mRNA expression in MG-63 cells. Finally, we employed the patch-clamp technique to show that an elevation in Cao 2+ as well as the specific, allosteric CaR activator NPS R-467 (0.5 μM), but not its less active stereoisomer NPS S-467 (0.5 μM), activate an outward K+ channel in MG-63 cells, strongly suggesting that the CaR in MG-63 cells is not only expressed but is functionally active.

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