scholarly journals CD44 (CD44 molecule (Indian blood group))

2011 ◽  
Author(s):  
YP Sen ◽  
GW Yip
Keyword(s):  
Blood Group ◽  
Indian Blood

Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in north Indian blood donors

2010 ◽  
Vol 43 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Beenu Thakral ◽  
Karan Saluja ◽  
Ratti Ram Sharma ◽  
Neelam Marwaha
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Indian Blood

Molecular genotyping of Indian blood group system antigens in Indian blood donors

2018 ◽  
Vol 57 (3) ◽  
pp. 388-390
Author(s):  
Harita Gogri ◽  
Pranali Pitale ◽  
Manisha Madkaikar ◽  
Swati Kulkarni
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Group System ◽  
Molecular Genotyping ◽  
Group System ◽  
Indian Blood

scholarly journals Novel variants in Krueppel Like Factor 1 that cause Persistence of Fetal Hemoglobin in In(Lu) individuals

2021 ◽  
Author(s):  
Jesse Eernstman ◽  
Barbera Veldhuisen ◽  
Peter Ligthart ◽  
Marieke von Lindern ◽  
Ellen van der Schoot ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Transcription Factor ◽  
Blood Group ◽  
High Frequency ◽  
Fetal Hemoglobin ◽  
Donor Group ◽  
Wild Type ◽  
Novel Variants ◽  
Klf1 Gene ◽  
Indian Blood

Abstract Beta-hemoglobinopathies become prominent after birth due to a switch from γ-globin to the mutated β-globin. Haploinsufficiency for the erythroid specific indispensable transcription factor Krueppel-like factor 1 (KLF1) is associated with high persistence of fetal hemoglobin (HPFH). The In(Lu) phenotype, characterized by low to undetectable Lutheran blood group expression is caused by mutations within KLF1 gene. These KLF1 variants often lead to KLF1 haploinsufficiency. We screened a donor cohort of 55 Lutheran weak or negative donors for KLF1 variants. To discriminate between weak and negative Lutheran expression, a flow cytometry (FCM) assay was developed to detect Lu polymorphisms. The Lu(a-b-) (negative) donor group, showing a significant decreased CD44 (Indian blood group) expression, also showed increased HbF and HbA2 levels, with outliers expressing >5% HbF. KLF1 exons and promoter sequencing revealed variants in 80% of the Lutheran negative donors. Thirteen different variants plus one high frequency SNP (c.304T>C) were identified of which 6 were novel. In primary erythroblasts, knockdown of endogenous KLF1 resulted in decreased CD44, Lu and increased HbF expression, while KLF1 over-expressing cells were comparable to wild type (WT). In line with the pleiotropic effects of KLF1 during erythropoiesis, distinct KLF1 mutants expressed in erythroblasts display different abilities to rescue CD44 and Lu expression and/or to affect fetal (HbF) or adult (HbA) hemoglobin expression.

Indian Blood Group System

2012 ◽  
pp. 567-575
Author(s):  
Marion E. Reid ◽  
Christine Lomas-Francis ◽  
Martin L. Olsson
Keyword(s):  
Blood Group ◽  
Blood Group System ◽  
Group System ◽  
Indian Blood

Molecular genotyping of Indian blood group antigens amongst regular voluntary blood donors of Surat city, Gujarat, India

2021 ◽  
pp. 103325
Author(s):  
Avani Shah ◽  
Parizad Patel ◽  
Keyuri Jariwala ◽  
Farzin Qureshi ◽  
Kanchan Mishra ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Group Antigens ◽  
Molecular Genotyping ◽  
Indian Blood

Two missense mutations in the CD44 gene encode two new antigens of the Indian blood group system

Transfusion ◽  
2007 ◽  
Vol 47 (7) ◽  
pp. 1306-1311 ◽  
Author(s):  
Joyce Poole ◽  
Louise Tilley ◽  
Nicole Warke ◽  
Frances A. Spring ◽  
Marijke A.M. Overbeeke ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Group System ◽  
Missense Mutations ◽  
Group System ◽  
Gene Encode ◽  
Indian Blood

scholarly journals A unique approach to screen for blood donors lacking high-prevalence antigen Inb of the Indian blood group system

2021 ◽  
Vol 37 (3) ◽  
pp. 126-130
Author(s):  
S.R. Joshi ◽  
S.B. Senjaliya ◽  
H.D. Maru ◽  
P.D. Kshirsagar ◽  
S.S. Kulkarni ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Group System ◽  
Group System ◽  
High Prevalence ◽  
Unique Approach ◽  
Indian Blood

Indian blood group system

2004 ◽  
pp. 455-460
Author(s):  
Marion E. Reid ◽  
Christine Lomas-Francis
Keyword(s):  
Blood Group ◽  
Blood Group System ◽  
Group System ◽  
Indian Blood

scholarly journals Novel variants in Krueppel like factor 1 that cause persistence of fetal hemoglobin in In(Lu) individuals

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jesse Eernstman ◽  
Barbera Veldhuisen ◽  
Peter Ligthart ◽  
Marieke von Lindern ◽  
C. Ellen van der Schoot ◽  
...  
Keyword(s):  
Blood Group ◽  
High Frequency ◽  
Target Gene ◽  
Fetal Hemoglobin ◽  
Antigen Expression ◽  
Donor Group ◽  
Wild Type ◽  
Blood Group Typing ◽  
Novel Variants ◽  
Indian Blood

AbstractBeta-hemoglobinopathies become prominent after birth due to a switch from γ-globin to the mutated β-globin. Haploinsufficiency for the erythroid specific indispensable transcription factor Krueppel-like factor 1 (KLF1) is associated with high persistence of fetal hemoglobin (HPFH). The In(Lu) phenotype, characterized by low to undetectable Lutheran blood group expression is caused by mutations within KLF1 gene. Here we screened a blood donor cohort of 55 Lutheran weak or negative donors for KLF1 variants and evaluated their effect on KLF1 target gene expression. To discriminate between weak and negative Lutheran expression, a flow cytometry (FCM) assay was developed to detect Lu antigen expression. The Lu(a−b−) (negative) donor group, showing a significant decreased CD44 (Indian blood group) expression, also showed increased HbF and HbA2 levels, with one individual expressing HbF as high as 5%. KLF1 exons and promoter sequencing revealed variants in 80% of the Lutheran negative donors. Thirteen different variants plus one high frequency SNP (c.304 T > C) were identified of which 6 were novel. In primary erythroblasts, knockdown of endogenous KLF1 resulted in decreased CD44, Lu and increased HbF expression, while KLF1 over-expressing cells were comparable to wild type (WT). In line with the pleiotropic effects of KLF1 during erythropoiesis, distinct KLF1 mutants expressed in erythroblasts display different abilities to rescue CD44 and Lu expression and/or to affect fetal (HbF) or adult (HbA) hemoglobin expression. With this study we identified novel KLF1 variants to be include into blood group typing analysis. In addition, we provide further insights into the regulation of genes by KLF1.

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