scholarly journals Detection of Culturable Bacteria from Tomato Seeds on Media Semi-selective for the Canker Pathogen

2021 ◽  
Vol 26 (1) ◽  
pp. 61-65
Author(s):  
NOBUTAKA SOMEYA ◽  
MASAHARU KUBOTA ◽  
TOMOHIRO MOROHOSHI ◽  
YASUHIRO INOUE
Keyword(s):  
Culturable Bacteria ◽  
Tomato Seeds ◽  
Canker Pathogen

Photodynamic inactivation to control Xanthomonas gardneri in tomato seeds

2021 ◽  
Author(s):  
Flávia de Souza Ferreira ◽  
Nilvanira Donizete Tebaldi ◽  
Carlos Alberto de Oliveira
Keyword(s):  
Photodynamic Inactivation ◽  
Tomato Seeds

scholarly journals Experimental studies to identify ultraviolet radiation impact on tomato seeds ‘Rozoviy Novichok” seeding quality

2021 ◽  
Vol 659 (1) ◽  
pp. 012035
Author(s):  
N E Ponomareva ◽  
G V Stepanchuk ◽  
N N Gracheva ◽  
I V Yudaev ◽  
N N Yakovenko ◽  
...  
Keyword(s):  
Ultraviolet Radiation ◽  
Experimental Studies ◽  
Tomato Seeds

scholarly journals Effect of Spaceflight on Tomato Seed Quality and Biochemical Characteristics of Mature Plants

Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 89
Author(s):  
Elena Dzhos ◽  
Nadezhda Golubkina ◽  
Marina Antoshkina ◽  
Irina Kondratyeva ◽  
Andrew Koshevarov ◽  
...  
Keyword(s):  
Seed Quality ◽  
Space Station ◽  
Field Conditions ◽  
Tomato Seed ◽  
Tomato Plants ◽  
Biochemical Characteristics ◽  
Tomato Seeds ◽  
Genetic Modifications ◽  
Total Antioxidant

Intensive space exploration includes profound investigations on the effect of weightlessness and cosmic radiation on plant growth and development. Tomato seeds are often used in such experiments though up to date the results have given rather vague information about biochemical changes in mature plants grown from seeds subjected to spaceflight. The effect of half a year of storage in the International Space Station (ISS) on tomato seeds (cultivar Podmoskovny ranny) was studied by analyzing the biochemical characteristics and mineral content of mature plants grown from these seeds both in greenhouse and field conditions. A significant increase was recorded in ascorbic acid, polyphenol and carotenoid contents, and total antioxidant activity (AOA), with higher changes in the field conditions compared to greenhouse. Contrary to control plants, the ones derived from space-stored seeds demonstrated a significant decrease in root AOA. The latter plants also showed a higher yield, but lower content of fruit dry matter, sugars, total dissolved solids and organic acids. The fruits of plants derived from space-stored seeds demonstrated decreased levels of Fe, Cu and taste index. The described results reflect the existence of oxidative stress in mature tomato plants as a long-term consequence of the effect of spaceflight on seed quality, whereas the higher yield may be attributed to genetic modifications.

scholarly journals Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications

2021 ◽  
Vol 9 (8) ◽  
pp. 1642
Author(s):  
Dorothee Tegtmeier ◽  
Sabine Hurka ◽  
Sanja Mihajlovic ◽  
Maren Bodenschatz ◽  
Stephanie Schlimbach ◽  
...  
Keyword(s):  
Gut Microbiome ◽  
Amplicon Sequencing ◽  
Industrial Applications ◽  
Culture Collection ◽  
Rrna Gene ◽  
Organic Substrates ◽  
Culturable Bacteria ◽  
Black Soldier Fly ◽  
Culture Independent ◽  
Culture Dependent

Black soldier fly larvae (BSFL) are fast-growing, resilient insects that can break down a variety of organic substrates and convert them into valuable proteins and lipids for applications in the feed industry. Decomposition is mediated by an abundant and versatile gut microbiome, which has been studied for more than a decade. However, little is known about the phylogeny, properties and functions of bacterial isolates from the BSFL gut. We therefore characterized the BSFL gut microbiome in detail, evaluating bacterial diversity by culture-dependent methods and amplicon sequencing of the 16S rRNA gene. Redundant strains were identified by genomic fingerprinting and 105 non-redundant isolates were then tested for their ability to inhibit pathogens. We cultivated representatives of 26 genera, covering 47% of the families and 33% of the genera detected by amplicon sequencing. Among these isolates, we found several representatives of the most abundant genera: Morganella, Enterococcus, Proteus and Providencia. We also isolated diverse members of the less-abundant phylum Actinobacteria, and a novel genus of the order Clostridiales. We found that 15 of the isolates inhibited at least one of the tested pathogens, suggesting a role in helping to prevent colonization by pathogens in the gut. The resulting culture collection of unique BSFL gut bacteria provides a promising resource for multiple industrial applications.

scholarly journals Development of β‐1,3‐glucanase activity in germinated tomato seeds

2000 ◽  
Vol 51 (349) ◽  
pp. 1381-1387 ◽  
Author(s):  
Yukio Morohashi ◽  
Hisashi Matsushima
Keyword(s):  
Glucanase Activity ◽  
Tomato Seeds

scholarly journals Evaluation of genetic integrity of tomato seeds during ageing by microsatellite markers

2014 ◽  
Vol 28 (2) ◽  
pp. 29-33 ◽  
Author(s):  
I.O. Daniel ◽  
O.W. Adabale ◽  
K.A. Adeboye ◽  
E.S. Aladele ◽  
O.T. Oduoye ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Integrity ◽  
Tomato Seeds

Method for the detachment of culturable bacteria from wetland gravel

2010 ◽  
Vol 80 (3) ◽  
pp. 242-250 ◽  
Author(s):  
Kela P. Weber ◽  
Raymond L. Legge
Keyword(s):  
Culturable Bacteria

scholarly journals Bacteria in the global atmosphere – Part 2: Modeling of emissions and transport between different ecosystems

2009 ◽  
Vol 9 (23) ◽  
pp. 9281-9297 ◽  
Author(s):  
S. M. Burrows ◽  
T. Butler ◽  
P. Jöckel ◽  
H. Tost ◽  
A. Kerkweg ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Residence Time ◽  
General Circulation ◽  
Bacterial Species ◽  
Circulation Model ◽  
Cloud Condensation Nucleus ◽  
Cloud Formation ◽  
Emission Region ◽  
Culturable Bacteria ◽  
Bacterial Cells

Abstract. Bacteria are constantly being transported through the atmosphere, which may have implications for human health, agriculture, cloud formation, and the dispersal of bacterial species. We simulate the global transport of bacteria, represented as 1 μm and 3 μm diameter spherical solid particle tracers in a general circulation model. We investigate factors influencing residence time and distribution of the particles, including emission region, cloud condensation nucleus activity and removal by ice-phase precipitation. The global distribution depends strongly on the assumptions made about uptake into cloud droplets and ice. The transport is also affected, to a lesser extent, by the emission region, particulate diameter, and season. We find that the seasonal variation in atmospheric residence time is insufficient to explain by itself the observed seasonal variation in concentrations of particulate airborne culturable bacteria, indicating that this variability is mainly driven by seasonal variations in culturability and/or emission strength. We examine the potential for exchange of bacteria between ecosystems and obtain rough estimates of the flux from each ecosystem by using a maximum likelihood estimation technique, together with a new compilation of available observations described in a companion paper. Globally, we estimate the total emissions of bacteria-containing particles to the atmosphere to be 7.6×1023–3.5×1024 a−1, originating mainly from grasslands, shrubs and crops. We estimate the mass of emitted bacteria- to be 40–1800 Gg a−1, depending on the mass fraction of bacterial cells in the particles. In order to improve understanding of this topic, more measurements of the bacterial content of the air and of the rate of surface-atmosphere exchange of bacteria will be necessary. Future observations in wetlands, hot deserts, tundra, remote glacial and coastal regions and over oceans will be of particular interest.

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