scholarly journals Research Article Human Serum Paraoxonase (PON-1) and hemochromatosis gene (HFE) gene polymorphisms in occupationally exposed lead workers from Saudi Arabia

2019 ◽  
Vol 18 (2) ◽  
Author(s):  
A.P. Shaik ◽  
A.H. Alsaeed ◽  
M. Faiyaz-ul-Haque ◽  
M.A. Alsaeed ◽  
A.S. Shaik
Keyword(s):  
Saudi Arabia ◽  
Human Serum ◽  
Gene Polymorphisms ◽  
Hfe Gene ◽  
Research Article ◽  
Hemochromatosis Gene ◽  
Occupationally Exposed ◽  
Lead Workers ◽  
Serum Paraoxonase

scholarly journals A critical review on human serum Paraoxonase-1 in the literature: truths and misconceptions

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Michael Mackness ◽  
Eser Yildirim Sozmen
Keyword(s):  
Heart Disease ◽  
Experimental Design ◽  
Human Serum ◽  
Critical Review ◽  
Neurological Diseases ◽  
Rapid Expansion ◽  
Paraoxonase 1 ◽  
Serum Paraoxonase ◽  
Inflammatory Component ◽  
Biomedical Fields

AbstractHuman serum paraoxonase 1 (PON1) appears to play an important role in the development of a large variety of diseases with an inflammatory component including heart disease, diabetes, rheumatic diseases, neurological diseases and cancer. As such PON1 research is rapidly expanding into new biomedical fields. Unfortunately, this rapid expansion has resulted in a number of problems due to poor experimental design and the spreading of misconceptions in the literature. This review seeks to describe the basic properties of PON1 and the problems and misconceptions that have arisen.

Properties of the retained N-terminal hydrophobic leader sequence in human serum paraoxonase/arylesterase

1999 ◽  
Vol 119-120 ◽  
pp. 243-249 ◽  
Author(s):  
Robert C. Sorenson ◽  
Michael Aviram ◽  
Charles L. Bisgaier ◽  
Scott Billecke ◽  
Cary Hsu ◽  
...  
Keyword(s):  
Human Serum ◽  
Leader Sequence ◽  
Serum Paraoxonase

scholarly journals Solid-Phase Amplification for Detection of C282Y and H63D Hemochromatosis (HFE) Gene Mutations

2001 ◽  
Vol 47 (8) ◽  
pp. 1384-1389 ◽  
Author(s):  
Mark S Turner ◽  
Sarah Penning ◽  
Angela Sharp ◽  
Valentine J Hyland ◽  
Ray Harris ◽  
...  
Keyword(s):  
Solid Phase ◽  
Point Mutations ◽  
Gene Mutations ◽  
Pcr Primers ◽  
Clinical Samples ◽  
Hfe Gene ◽  
Target Sequence ◽  
Nucleotide Polymorphisms ◽  
Nitrophenyl Phosphate ◽  
Hemochromatosis Gene

Abstract Background: There is a need for simple, rapid, and inexpensive methods for the detection of single-nucleotide polymorphisms. Our aim was to develop a single-tube ELISA-like PCR assay and evaluate it by detecting the common C282Y and H63D mutations found in the hemochromatosis gene (HFE) by use of clinical samples. Methods: The method, termed solid-phase amplification (SPA), involves dual liquid- and solid-phase amplification of a target sequence by the use of two PCR primers, one of which is in two forms: the first is covalently immobilized to the wall of a microwell, and the second is free in solution. During allele-specific amplification, both the free and solid-phase amplicons are labeled by incorporation of digoxigenin (DIG)-dUTP. The amount of surface-bound amplicon is determined colorimetrically by the use of an alkaline phosphatase-anti-DIG-Fab conjugate and p-nitrophenyl phosphate. Results: Two different amplicon-labeling methods were evaluated. Analysis of 173 clinical samples for the C282Y and H63D HFE point mutations with SPA revealed that only one sample was incorrectly diagnosed, apparently because of operator error, when compared with conventional restriction fragment length polymorphism assay results. Conclusions: The SPA assay has potential for medium-scale mutation detection, having the advantage of being manipulatively simple and immediately adaptable for use in clinical laboratories with existing ELISA instrumentation.

Association of human serum paraoxonase‐1 with some respiratory drugs

2019 ◽  
Vol 33 (12) ◽  
Author(s):  
Başak Gökçe ◽  
Nurhan Sarıoğlu ◽  
Nahit Gençer ◽  
Oktay Arslan
Keyword(s):  
Human Serum ◽  
Paraoxonase 1 ◽  
Serum Paraoxonase ◽  
Respiratory Drugs

scholarly journals Novel Nucleophiles Enhance the Human Serum Paraoxonase 1 (PON1)-mediated Detoxication of Organophosphates

2014 ◽  
Vol 143 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Janice E. Chambers ◽  
Howard W. Chambers ◽  
Edward C. Meek ◽  
Kristen E. Funck ◽  
Manikanthan H. Bhavaraju ◽  
...  
Keyword(s):  
Human Serum ◽  
Paraoxonase 1 ◽  
Serum Paraoxonase

scholarly journals Human Serum Paraoxonase/Arylesterase’s Retained HydrophobicN-Terminal Leader Sequence Associates With HDLs by Binding Phospholipids

1999 ◽  
Vol 19 (9) ◽  
pp. 2214-2225 ◽  
Author(s):  
Robert C. Sorenson ◽  
Charles L. Bisgaier ◽  
Michael Aviram ◽  
Cary Hsu ◽  
Scott Billecke ◽  
...  
Keyword(s):  
Human Serum ◽  
Leader Sequence ◽  
Serum Paraoxonase

Iron metabolism and the role of HFE gene polymorphisms in Wilson disease

2011 ◽  
Vol 32 (1) ◽  
pp. 165-170 ◽  
Author(s):  
Jan Pfeiffenberger ◽  
Daniel N. Gotthardt ◽  
Thomas Herrmann ◽  
Jessica Seeßle ◽  
Uta Merle ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Wilson Disease ◽  
Gene Polymorphisms ◽  
Hfe Gene
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