scholarly journals Research Article Genetic diversity in natural populations of Stylosanthes scabra Fabaceae using ISSR markers

2019 ◽  
Vol 18 (2) ◽  
Author(s):  
J.C. Costa ◽  
G.G.M. Fracetto ◽  
F.J.C. Fracetto ◽  
T.C. Souza ◽  
M.V.F. Santos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Natural Populations ◽  
Issr Markers ◽  
Research Article

scholarly journals Research Article Genetic diversity in cowpea landraces analyzed by ISSR markers

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
L.B.R. Araújo ◽  
L.B.C. Fiege ◽  
A.V.A Silva ◽  
C.H.C.M. Bertini
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Research Article

scholarly journals Research Article Genetic diversity in a cajuí (Anacardium spp.) germplasm bank as determined by ISSR markers

2018 ◽  
Vol 17 (4) ◽  
Author(s):  
A.N.C. Borges ◽  
A.C.A. Lopes ◽  
F.B. Britto ◽  
L.F.L. Vasconcelos ◽  
P.S.C. Lima
Keyword(s):  
Genetic Diversity ◽  
Issr Markers ◽  
Germplasm Bank ◽  
Research Article

Genetic diversity in natural populations of Colossomamacropomum in the Brazilian Amazon region and in populations farmed in Northeast Brazil based on ISSR markers

2019 ◽  
Vol 27 (5) ◽  
pp. 1423-1434
Author(s):  
Claudivane de Sá Teles Oliveira ◽  
Ricardo Franco Cunha Moreira ◽  
Aldeney Andrade Soares Filho ◽  
Soraia Barreto Aguiar Fonteles ◽  
Norma Suely Evangelista-Barreto
Keyword(s):  
Genetic Diversity ◽  
Natural Populations ◽  
Brazilian Amazon ◽  
Issr Markers ◽  
Amazon Region ◽  
Northeast Brazil ◽  
Brazilian Amazon Region

Genetic diversity of natural populations of medicinally valuable plant Satureja khuzistanica Jamzad based on ISSR markers

2017 ◽  
Vol 40 (3) ◽  
pp. 771-781 ◽  
Author(s):  
Javad Hadian ◽  
Safiollah Raeisi ◽  
Ali Azizi ◽  
Maryam Pezhmanmehr ◽  
Ali Sarkhosh
Keyword(s):  
Genetic Diversity ◽  
Natural Populations ◽  
Issr Markers

scholarly journals Low Divergence Among Natural Populations of Cornus kousa subsp. chinensis Revealed by ISSR Markers

Forests ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1082 ◽  
Author(s):  
Jia-Qiu Yuan ◽  
Qin Fang ◽  
Guo-Hua Liu ◽  
Xiang-Xiang Fu
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
In Situ Conservation ◽  
Geographic Distance ◽  
Natural Populations ◽  
Issr Markers ◽  
Mantel Test ◽  
Distribution Area ◽  
Genetic Characteristics ◽  
Cornus Kousa

Research Highlights: Taking Chinese dogwood (Cornus kousa subsp. chinensis) as an example, the genetic characteristics of natural populations collected from main a distribution area were evaluated using intersimple sequence repeat (ISSR) markers to reveal the genetic basis for further selection and breeding. Background and Objectives: Chinese dogwood is a small understory tree that is widely distributed in China. Chinese dogwood has attracted interest for its potential horticultural and ornamental values, and its natural resource potential urgently needs to be estimated. Materials and Methods: In this study, the genetic diversity of 12 natural populations collected from six provinces containing 223 individuals was evaluated based on ISSR markers. Results: Relatively high levels of genetic diversity were found at both the population and individual levels. The Shannon’s diversity index (I) among individuals (0.504) was higher than that among populations (0.338). Analysis of molecular variance (AMOVA) revealed that genetic variation mainly existed within populations (61.55%) rather than among populations (38.45%). According to the STRUCTURE analysis, 12 populations were assigned to two groups, i.e., the northern and southern ecological regions, which are separated by the Yangtze River. A Mantel test analysis showed that there was no significant correlation between genetic distance and geographic distance. Conclusions: Considering the breeding system of dogwoods, we speculated that the genetic characteristics of the natural populations of this species would be affected by the dispersal mode of its pollens and seeds; additionally, genetic drift could play an important role in its genetic differentiation. In conclusion, in situ conservation is recommended for Chinese dogwood based on our results.

Assessing the genetic diversity of Myrsine umbellata (Primulaceae) in Brazilian Atlantic Forest remnants - an important step towards reforestation efforts

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Micheli Sossai Spadeto ◽  
Thais Lazarino Maciel ◽  
Tatiana Tavares Carrijo ◽  
Marcia Flores da Silva Ferreira ◽  
Milene Miranda Praça Fontes
Keyword(s):  
Genetic Diversity ◽  
Atlantic Forest ◽  
Native Species ◽  
Natural Populations ◽  
Production Capacity ◽  
Issr Markers ◽  
Isolated Populations ◽  
Forest Remnants ◽  
High Production

Abstract The investigation of genetic diversity in natural populations of species that show potential for use in reforestation programs is a key step in making management decisions. However, reforestation programs with native species in Brazil are still rarely based on a genetic understanding of the seed matrices used for seedling production. This is also the case for Myrsine umbellata, a dioecious shrub within the family Primulaceae that has been used in reforestation programs in Brazil, mainly due to its high production capacity of fruits attractive to the avifauna. The goal of this study was to measure intra- and interpopulational genetic diversity in natural populations of M. umbellata in six forest remnants of the Atlantic Forest using ISSR markers. The results revealed that the intrapopulational genetic diversity was greater than the genetic diversity among the studied populations. For this reason, the cultivation of seedlings from seeds obtained in more than one population seems the most appropriate strategy for reforestation purposes. Even though the most isolated populations are also the ones with highest genetic structure, all populations of M. umbellata included in this study revealed to be an important germplasm bank conserved in situ.

scholarly journals ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations

2021 ◽  
Vol 38 ◽  
pp. 00082
Author(s):  
Dinara S. Muraseva ◽  
Alexandra A. Guseva
Keyword(s):  
Genetic Diversity ◽  
Nuclear Dna ◽  
Natural Populations ◽  
Issr Markers ◽  
Population Diversity ◽  
Point Scale ◽  
High Quality ◽  
Issr Primer ◽  
Issr Analysis ◽  
Selection Of

Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was assessed on a point scale. When assessing the primers, the number of reproducible amplified DNA fragments, the clarity and brightness of the obtained fragments, and only distinctive bands were taken into account. As a result, 10 ISSR markers were selected that are the most informative for assessing the population diversity of the species.

Genetic diversity and structure among natural populations of Sindora glabra in Hainan Island, China as revealed by ISSR markers

2016 ◽  
Vol 69 ◽  
pp. 145-151 ◽  
Author(s):  
Jin-chang Yang ◽  
Qiong-Qiong Li ◽  
Niu Yu ◽  
Guang-tian Yin ◽  
Zhong-feng Wu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Natural Populations ◽  
Hainan Island ◽  
Issr Markers ◽  
Genetic Diversity And Structure

scholarly journals Study of genetic diversity and molecular variation in natural populations of Pinus armandii subsp. Xuannhaensis L.K. Phan - an endemic species in Son La, Vietnam using ISSR markers

2016 ◽  
Vol 14 (3) ◽  
pp. 441-450 ◽  
Author(s):  
Vũ Đình Duy ◽  
Bùi Thị Tuyết Xuân ◽  
Đỗ Thị Phương Thảo ◽  
Phan Kế Lộc ◽  
Nguyễn Minh Tâm
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Nature Reserve ◽  
Natural Populations ◽  
Population Level ◽  
Issr Markers ◽  
Similarity Matrix ◽  
Similarity Coefficients ◽  
Analysis Of Molecular Variance ◽  
Narrow Endemic

Pinus armandii subsp. xuannhaensis L.K. Phan is a new five needle pine discovered recently from Xuan Nha Nature Reserve, Son La province. This subspecies is considered as a narrow endemic to Vietnam and is assessed as endangered. In this study, 15 ISSR markers were used to analyze the genetic diversity of this taxon collected in five subpopulations (Tan Xuan, Thac Nuoc, Dinh VTV2, Near VTV2 and Dinh Pomu). Results of the analysis showed 15/15 markers were polymorphic. A total of 51 DNA fragments were amplified, in which 50 fragments were polymorphic (98.04%). Genetic diversity was the highest in Dinh Pomu subpopulation (I = 0.555; h = 0.8; PPB = 68.76%; Ne =1.6 and He = 0.4)) and the lowest in Tan Xuan subpopulation (I = 0.428; h = 0.6; PPB = 57.06%, Ne = 1.215 and He = 0.303). Analysis of molecular variance (AMOVA) results showed that the total level of molecular changes between subpopulations was 7% and between individuals in the same subpopulation was 93%. A constructed dendrogram based on similarity matrix of 71 Pinus armandii subsp. xuannhaensis L.K. Phan samples divided the samples into two main groups with genetic similarity coefficients ranged from 0.53 to 0.96. Results of the molecular analysis showed that Pinus armandii subsp. xuannhaensis L.K. Phan species should be protected at the population level.

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