scholarly journals Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

2015 ◽  
Vol 14 (1) ◽  
pp. 2890-2899 ◽  
Author(s):  
C. Gutiérrez-Vergara ◽  
J. Quintero ◽  
J.F. Duarte ◽  
J.P. Suescún ◽  
A. López-Herrera
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome

Hepatitis E virus genome in stools of hepatitis patients during large epidemic in north India

The Lancet ◽  
1991 ◽  
Vol 338 (8770) ◽  
pp. 783-784 ◽  
Author(s):  
R. Ray ◽  
G.P. Talwar ◽  
R. Aggarwal ◽  
P.N. Salunke ◽  
S.R. Naik ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome ◽  
North India

An analysis of two open reading frames (ORF3 and ORF4) of rat hepatitis E virus genome using its infectious cDNA clones with mutations in ORF3 or ORF4

2018 ◽  
Vol 249 ◽  
pp. 16-30 ◽  
Author(s):  
Tanggis ◽  
Tominari Kobayashi ◽  
Masaharu Takahashi ◽  
Suljid Jirintai ◽  
Tsutomu Nishizawa ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome ◽  
Open Reading Frames ◽  
Cdna Clones ◽  
Reading Frames

scholarly journals Hepatitis E virus genome detection in commercial pork livers and pork meat products in Germany

2020 ◽  
Vol 28 (1) ◽  
pp. 196-204 ◽  
Author(s):  
Srinivas Reddy Pallerla ◽  
Sonja Schembecker ◽  
Christian G. Meyer ◽  
Le Thi Kieu Linh ◽  
Reimar Johne ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Meat Products ◽  
Hepatitis E ◽  
Virus Genome ◽  
Pork Meat

scholarly journals The Nucleotides on the Stem-Loop RNA Structure in the Junction Region of the Hepatitis E Virus Genome Are Critical for Virus Replication

2010 ◽  
Vol 84 (24) ◽  
pp. 13040-13044 ◽  
Author(s):  
Dianjun Cao ◽  
Yao-Wei Huang ◽  
Xiang-Jin Meng
Keyword(s):  
Virus Replication ◽  
Intergenic Region ◽  
Rna Structure ◽  
Hepatitis E Virus ◽  
Reporter Genes ◽  
Hepatitis E ◽  
Virus Genome ◽  
Junction Region ◽  
Stem Loop ◽  
Huh7 Cells

ABSTRACT The roles of conserved nucleotides on the stem-loop (SL) structure in the intergenic region of the hepatitis E virus (HEV) genome in virus replication were determined by using Huh7 cells transfected with HEV SL mutant replicons containing reporter genes. One or two nucleotide mutations of the AGA motif on the loop significantly reduced HEV replication, and three or more nucleotide mutations on the loop abolished HEV replication. Mutations on the stem and of the subgenome start sequence also significantly inhibited HEV replication. The results indicated that both the sequence and the SL structure in the junction region play important roles in HEV replication.

scholarly journals NMR study on the interaction of the conserved CREX ‘stem–loop’ in the Hepatitis E virus genome with a naphthyridine-based ligand

2015 ◽  
Vol 13 (37) ◽  
pp. 9665-9672 ◽  
Author(s):  
N. Dyubankova ◽  
M. Froeyen ◽  
M. Abramov ◽  
H. P. Mattelaer ◽  
P. Herdewijn ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome ◽  
Stem Loop ◽  
Dna And Rna ◽  
Nmr Study ◽  
Rna Duplexes

A 2-amino-1,8-naphthyridine derivative that is described to bind single guanine bulges in RNA–DNA and RNA–RNA duplexes was synthesized and its interaction with the single G bulge in the conserved CREX of the Hepatitis E Virus (HEV) genome was explored by NMR and molecular modeling.

scholarly journals Detection and quantification of hepatitis E virus genome in pig liver samples originating from Serbian retail establishments

2021 ◽  
Vol 854 (1) ◽  
pp. 012059
Author(s):  
L Milojevic ◽  
B Velebit ◽  
M Dimitrijevic ◽  
V Djordjevic ◽  
V Jankovic ◽  
...  
Keyword(s):  
Potential Risk ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Viral Disease ◽  
Virus Genome ◽  
Target Tissue ◽  
Pig Liver ◽  
Wild Pigs ◽  
Four Seasons ◽  
One Year

Abstract Hepatitis E is considered an emerging human viral disease with a zoonotic nature, and domestic and wild pigs are the main reservoirs of hepatitis E virus (HEV) among animals. Pork liver is the target tissue of this virus. This study aimed to investigate the presence of HEV in commercial pig liver samples. Sixty samples were collected during one year from different retail outlets in Serbia. Furthermore, the collected samples were separated by four seasons, and every season included three months. The presence of HEV in the livers was examined by molecular analysis using RT-qPCR. The overall prevalence of the virus in analysed pig livers was 5%. HEV was detected in three livers, two in the first season and one in the second, while in the third and fourth season, no positive livers were detected. However, there were no statistically significant differences between the surveyed seasons. HEV was quantified in positive livers. Among positive livers, HEV concentrations ranged between 8×101 and 1.9×104 genome copies of the virus per gram. The presence of HEV in commercial pig livers indicates a potential risk for consumers. Appropriate heat treatment of meals during preparation is essential to eliminate the potential risk of developing the illness.

scholarly journals Evolution of the hepatitis E virus hypervariable region

2012 ◽  
Vol 93 (11) ◽  
pp. 2408-2418 ◽  
Author(s):  
Donald B. Smith ◽  
Jeff Vanek ◽  
Sandeep Ramalingam ◽  
Ingolfur Johannessen ◽  
Kate Templeton ◽  
...  
Keyword(s):  
Amino Acid ◽  
Positive Selection ◽  
Hepatitis E Virus ◽  
Hypervariable Region ◽  
Hepatitis E ◽  
Virus Genome ◽  
Amino Acid Sequences ◽  
Specific Sequence ◽  
Virus Genotypes ◽  
Selection For

The presence of a hypervariable (HVR) region within the genome of hepatitis E virus (HEV) remains unexplained. Previous studies have described the HVR as a proline-rich spacer between flanking functional domains of the ORF1 polyprotein. Others have proposed that the region has no function, that it reflects a hypermutable region of the virus genome, that it is derived from the insertion and evolution of host sequences or that it is subject to positive selection. This study attempts to differentiate between these explanations by documenting the evolutionary processes occurring within the HVR. We have measured the diversity of HVR sequences within acutely infected individuals or amongst sequences derived from epidemiologically linked samples and, surprisingly, find relative homogeneity amongst these datasets. We found no evidence of positive selection for amino acid substitution in the HVR. Through an analysis of published sequences, we conclude that the range of HVR diversity observed within virus genotypes can be explained by the accumulation of substitutions and, to a much lesser extent, through deletions or duplications of this region. All published HVR amino acid sequences display a relative overabundance of proline and serine residues that cannot be explained by a local bias towards cytosine in this part of the genome. Although all published HVRs contain one or more SH3-binding PxxP motifs, this motif does not occur more frequently than would be expected from the proportion of proline residues in these sequences. Taken together, these observations are consistent with the hypothesis that the HVR has a structural role that is dependent upon length and amino acid composition, rather than a specific sequence.

scholarly journals In vivo evidence for ribavirin-induced mutagenesis of the hepatitis E virus genome

Gut ◽  
2016 ◽  
Vol 65 (10) ◽  
pp. 1733-1743 ◽  
Author(s):  
Daniel Todt ◽  
Anett Gisa ◽  
Aleksandar Radonic ◽  
Andreas Nitsche ◽  
Patrick Behrendt ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genome ◽  
Induced Mutagenesis

scholarly journals Phylogenetic Analysis and Characterization of the Complete Hepatitis E Virus Genome (Zoonotic Genotype 3) in Swine Samples from Mexico

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 391 ◽  
Author(s):  
Alicia Sotomayor-González ◽  
María Trujillo-Ortega ◽  
Blanca Taboada-Ramírez ◽  
Carlos Sandoval-Jaime ◽  
Rosa Sarmiento-Silva
Keyword(s):  
Public Health ◽  
Hepatitis E Virus ◽  
Public Health Problem ◽  
Hepatitis E ◽  
Virus Genome ◽  
Complete Sequence ◽  
Farm Animals ◽  
Genotype 3 ◽  
Bile Sample ◽  
Genotype 2

Hepatitis E virus (HEV) is an emerging public health problem with an estimated 20 million infections each year. In Mexico, Orthohepevirus A, genotype 2, has been reported in humans, but genotype 3 has only been reported in swine (zoonotic). No diagnostic tests are publicly available in Mexico, and only partial sequences have been reported from swine samples. Hence, research is necessary to determine circulating strains, understand the features and dynamics of infection on pig farms, determine how to implement surveillance programs, and to assess public health risks. In this study, a next-generation sequencing (NGS) approach was applied to obtain a complete genome of swine HEV. Liver, feces, and bile samples were taken at slaughterhouses and a farm in Mexico. RT-PCR was used to determine positive samples and confirmed by Sanger sequencing. Of the 64 slaughterhouse samples, one bile sample was positive (B1r) (1.56%). Of 21 sample pools from farm animals, 14 were positive (66.66%), representing all stages of production. A complete sequence strain MXCDg3_B1c|_2016 was obtained from the bile of a domestic swine in the fattening stage. In addition, two partial sequences—MXCDg3_H2cons|_2016 (1473 nt) and MXCDg3_C3Acons|_2016 (4777 nt)—were obtained from sampled farm animals. Comparison with all reported genome HEV sequences showed similarity to genotype 3 subgenotype a (G3a), which has been previously reported in acute cases of human hepatitis in the US, Colombia, China, and Japan.

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