scholarly journals Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress

2015 ◽  
Vol 14 (1) ◽  
pp. 2384-2398 ◽  
Author(s):  
G.P. Moraes ◽  
L.C. Benitez ◽  
M.N. do Amaral ◽  
I.L. Vighi ◽  
P.A. Auler ◽  
...  
Keyword(s):  
Salt Stress ◽  
Reference Genes ◽  
Rice Seedlings

Melatonin Regulates Antioxidant Strategy in Response to Continuous Salt Stress in Rice Seedlings

2021 ◽  
Author(s):  
Feiyu Yan ◽  
Haimin Wei ◽  
Yanfeng Ding ◽  
Weiwei Li ◽  
Zhenghui Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Rice Seedlings

Identification and validation of reference genes for quantitative real-time PCR under salt stress in a halophyte, Sesuvium portulacastrum

Plant Gene ◽  
2018 ◽  
Vol 13 ◽  
pp. 18-24 ◽  
Author(s):  
Ganesh C. Nikalje ◽  
Ashish K. Srivastava ◽  
Gaurav Sablok ◽  
Girdhar K. Pandey ◽  
Tukaram D. Nikam ◽  
...  
Keyword(s):  
Salt Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Sesuvium Portulacastrum

scholarly journals Selection of reference genes for quantitative real-time PCR in Casuarina equisetifolia under salt stress

2017 ◽  
Vol 61 (3) ◽  
pp. 463-472 ◽  
Author(s):  
C. Fan ◽  
Z. Qiu ◽  
B. Zeng ◽  
Y. Liu ◽  
X. Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Casuarina Equisetifolia ◽  
Quantitative Real Time Pcr ◽  
Selection Of

scholarly journals Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization inIris. lacteavar.chinensisRoots under Cadmium, Lead, and Salt Stress Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Chun-Sun Gu ◽  
Liang-qin Liu ◽  
Chen Xu ◽  
Yan-hai Zhao ◽  
Xu-dong Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Real Time ◽  
Reference Genes ◽  
Elongation Factor ◽  
Stress Conditions ◽  
Translation Initiation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range

Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples. In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1α), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), inIris. lacteavar.chinensis(I. lacteavar.chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions. All seven reference genes showed a relatively wide range of threshold cycles (Ct) values in different samples. GeNorm and NormFinder algorithms were used to assess the suitable reference genes. The results from the two software units showed thatEIF-5AandUBCwere the most stable reference genes across all of the tested samples, whileTUBLINwas unsuitable as internal controls.I. lacteavar.chinensisis tolerant to Cd, Pb, and salt. Our results will benefit future research on gene expression in response to the three abiotic stresses.

scholarly journals   Effects of exogenous proline and trehalose on physiological responses in rice seedlings during salt-stress and after recovery

2012 ◽  
Vol 58 (No. 7) ◽  
pp. 309-315 ◽  
Author(s):  
N. Nounjan ◽  
P. Theerakulpisut
Keyword(s):  
Antioxidant Enzymes ◽  
Salt Stress ◽  
Physiological Responses ◽  
Recovery Period ◽  
Growth Enhancement ◽  
Salt Tolerant ◽  
Rice Seedlings ◽  
Growth Recovery ◽  
Growth Increase ◽  
High Level

Two osmoprotectants (proline &ndash; Pro; trehalose &ndash; Tre) were exogenously supplied to seedlings of rice cvs. Pokkali (PK &ndash; salt-tolerant) and Khao Dawk Mali 105 (KDML105 &ndash; salt-sensitive) to investigate their effects on plants exposed to 200 mmol/L NaCl for 6 days and 5 days after recovery from stress. The reduction of growth, increase in Na<sup>+</sup> to K<sup>+</sup> ratio, high level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) content, enhanced activity of antioxidant enzymes (superoxide dismutase &ndash; SOD, peroxidase &ndash; POX, catalase &ndash; CAT and ascorbate peroxidase &ndash; APX) were observed in both rice cultivars under NaCl treatments. Exogenous Pro and Tre supplement to NaCl-stressed plants did not mitigate the reduction of growth during salt-stress. Nevertheless, during recovery plants previously supplied with Tre showed markedly higher percentage of growth recovery than those treated with NaCl alone or supplied with Pro. The beneficial effect of Tre on growth recovery was clearly demonstrated in KDML105 in which growth enhancement was related to reduction in Na<sup>+</sup> to K<sup>+</sup> ratio. Exogenous Pro was able to reduce H<sub>2</sub>O<sub>2</sub> in both cultivars during salt stress whereas Tre could reduce it only in KDML105. Exogenous Tre did not enhance any antioxidant enzymes during stress but enhanced APX activity in KDML105 during recovery. Exogenous Pro enhanced the activity of APX in PK, and POX, CAT and APX in KDML105 during both stress and recovery period. &nbsp; &nbsp;

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