scholarly journals Identification of QTLs of resistance to white mold in common bean from multiple markers by using Bayesian analysis

2015 ◽  
Vol 14 (1) ◽  
pp. 1124-1135 ◽  
Author(s):  
L.A.C. Lara ◽  
J.B. Santos ◽  
M. Balestre ◽  
I.A. Lima ◽  
A.K.A. Pamplona ◽  
...  
Keyword(s):  
Bayesian Analysis ◽  
Common Bean ◽  
White Mold ◽  
Multiple Markers

White Mold–Resistant Interspecific Common Bean Germplasm Lines VCW 54 and VCW 55

2009 ◽  
Vol 3 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Shree P. Singh ◽  
Henry Terán ◽  
Howard F. Schwartz ◽  
Kristen Otto ◽  
Margarita Lema
Keyword(s):  
Common Bean ◽  
White Mold

Efficacy of three greenhouse screening methods for the identification of physiological resistance to white mold in dry bean

2009 ◽  
Vol 89 (4) ◽  
pp. 755-762 ◽  
Author(s):  
H Terán ◽  
S P Singh
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Screening Method ◽  
White Mold ◽  
Screening Methods ◽  
Post Inoculation ◽  
Dry Bean ◽  
Physiological Resistance ◽  
Nueva Granada

White mold (WM) caused by Sclerotinia sclerotiorum (Lib.) de Bary is the most devastating disease of common bean (dry and snap or garden bean) (Phaseolus vulgaris L.) in North America. The use of a reliable screening method (SM) in common bean is crucial to improve physiological resistance to WM. The objective of this study was to compare the efficacy of three SM to identify physiological resistance in dry bean genotypes with different evolutionary origins and levels of resistance. Screening methods tested were: (i) the modified straw test or cut–stem (CSM); (ii) infected bean flower (IFL); and (iii) infected oat seed (IOS). A 195, ICA Bunsi, Othello, and VCW 54 dry bean were tested with the three SM. The experimental design was a split plot in randomized complete blocks with three replications in 2007 and 2008. Two independent inoculations 1 wk apart for each SM were made. The WM reaction was scored at 16, 23, and 33 d post-inoculation (DPI) using a 1 to 9 scale. There were highly significant differences between SM and its interaction with years. The CSM and IFL were the most consistent and highly correlated (r > 0.70, P < 0.01). Interspecific breeding line VCW 54 consistently had the highest WM resistance across years, SM, and evaluation dates, followed by A 195. White mold scores increased with delayed evaluations. Thus, CSM or IFL with disease assessed 33 DPI should be used for identifying common bean genotypes with high levels of physiological resistance to WM.Key words: Common bean, growth habit, race Mesoamerica, race Nueva Granada, Phaseolus vulgaris, Sclerotinia sclerotiorum

Comparison of inoculation methods for selecting common bean genotypes with physiological resistance to white mold

2018 ◽  
Vol 44 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Lenio U. Ferreira ◽  
Victor A. Ribeiro ◽  
Patrícia G. S. Melo ◽  
Murillo Lobo Junior ◽  
Joaquim Geraldo C. Costa ◽  
...  
Keyword(s):  
Common Bean ◽  
White Mold ◽  
Inoculation Methods ◽  
Physiological Resistance

scholarly journals Protocol adjustment improves the extraction of high-quality total RNA from common bean stems infected by Sclerotinia sclerotiorum

2019 ◽  
Vol 43 ◽  
Author(s):  
Rafael Novais de Miranda ◽  
Caroline Marcela da Silva ◽  
Antonio Carlos da Mota Porto ◽  
Welison Andrade Pereira
Keyword(s):  
Gene Expression ◽  
Common Bean ◽  
Sclerotinia Sclerotiorum ◽  
Rna Extraction ◽  
White Mold ◽  
Basic Requirement ◽  
High Quality ◽  
Commercial Kits ◽  
Made In

ABSTRACT The Straw Test is an assay developed to evaluate the resistance of common bean to white mold, in which the plant stems are inoculated and the symptoms of the disease are monitored. It is plausible to admit that investigating gene expression in pathogen-infected tissues may be strategically interesting. However, obtaining a quality RNA is a basic requirement for this purpose. Therefore, the objective of this study was to evaluate adjustments in protocols of commercial kits in the expectation of improving the quality of RNA obtained from bean stems. For this, plants of two lines were inoculated and the stems pathogen-infected were collected 72 hours after. For RNA extraction, two commercial reagents were used following the manufacturer’s recommendations and then following adaptations in these protocols. In particular, the proposed modifications relate to volumes of supernatant recovered in purification steps, additional step of chloroform purification and extended time for nucleic acids precipitation. The obtained RNA was analyzed by spectrophotometer, electrophoresis and bioanalyzer, then converted into cDNA and subsequently submitted to PCR. From the obtained data, it was observed that the adaptations made in the protocols contributed to better results and that, when the indicative values of RNA quality are guaranteed, the subsequent reactions are more pure, precise and representative.

Identification of QTL Associated with White Mold Resistance in Common Bean

Crop Science ◽  
2005 ◽  
Vol 45 (6) ◽  
pp. 2482-2490 ◽  
Author(s):  
Marcio Ender ◽  
James D. Kelly
Keyword(s):  
Common Bean ◽  
White Mold ◽  
Mold Resistance

scholarly journals Combining ability as a strategy for selecting common bean parents and populations resistant to white mold

2018 ◽  
Vol 18 (3) ◽  
pp. 276-283 ◽  
Author(s):  
Lenio Urzeda Ferreira ◽  
Patrícia Guimarães Santos Melo ◽  
Rogério Faria Vieira ◽  
Murillo Lobo Junior ◽  
Helton Santos Pereira ◽  
...  
Keyword(s):  
Common Bean ◽  
Combining Ability ◽  
White Mold

Mapping of QTL for Resistance to White Mold Disease in Common Bean

Crop Science ◽  
2001 ◽  
Vol 41 (4) ◽  
pp. 1253-1262 ◽  
Author(s):  
Soon O. Park ◽  
Dermot P. Coyne ◽  
James R. Steadman ◽  
Paul W. Skroch
Keyword(s):  
Common Bean ◽  
White Mold

scholarly journals White Mold Management in Common Bean by Increasing Within-Row Distance Between Plants

Plant Disease ◽  
2010 ◽  
Vol 94 (3) ◽  
pp. 361-367 ◽  
Author(s):  
R. F. Vieira ◽  
T. J. Paula Júnior ◽  
H. Teixeira ◽  
J. E. de S. Carneiro
Keyword(s):  
Common Bean ◽  
Winter Season ◽  
Sprinkler Irrigation ◽  
Severity Index ◽  
White Mold ◽  
Row Spacing ◽  
Plant Distributions ◽  
Growing Seasons ◽  
Indeterminate Growth Habit ◽  
Flowering Onset

White mold of common bean (Phaseolus vulgaris), caused by Sclerotinia sclerotiorum, is a major yield-limiting disease during the fall-winter season in Brazil. This study was conducted to evaluate the efficacy of decreasing within-row densities for an indeterminate growth habit (type IIIa) cultivar, keeping constant the between-row spacing of 0.5 m, to manage the disease. A modification of within-row plant distributions was also attempted in order to reduce white mold intensity. The study was conducted with sprinkler irrigation in two growing seasons (2000 and 2001) in Viçosa, State of Minas Gerais, Brazil, in an area naturally infested with sclerotia. In 2000, treatments were arranged as 3 × 2 × 2 factorial combination of within-row densities (15, 7.5, or 5 plants/m), within-row plant distributions (single and equidistant plants or equidistant groups of three plants), and fluazinam treatments (with or without). In 2001, 16, 12, 8, or 4 plants/m were combined with fluazinam treatments. Fluazinam was applied at both flowering onset and 10 or 13 days later. Average incidence was 92.6% in 2000 and 77.8% in 2001; severity index was 69.8% in 2000 and 40.2% in 2001; and yield was 1,656 kg/ha in 2000 and 2,542 kg/ha in 2001. White mold decreased and yield increased as within-row densities were reduced, regardless of fluazinam treatments. A distribution of equidistant groups of three plants was ineffective in reducing disease. The use of 4 equidistant plants/m in infested irrigated areas was an effective strategy in white mold management.

Mapping quantitative trait loci conferring partial physiological resistance to white mold in the common bean RIL population Xana × Cornell 49242

2010 ◽  
Vol 29 (1) ◽  
pp. 31-41 ◽  
Author(s):  
Elena Pérez-Vega ◽  
Aida Pascual ◽  
Ana Campa ◽  
Ramón Giraldez ◽  
Phillip N. Miklas ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Common Bean ◽  
Quantitative Trait ◽  
White Mold ◽  
Ril Population ◽  
Trait Loci ◽  
Physiological Resistance ◽  
The Common

scholarly journals Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Letícia A. de C. Lara ◽  
João B. dos Santos ◽  
Juliana S. Veloso ◽  
Marcio Balestre ◽  
Filipe C. Alves ◽  
...  
Keyword(s):  
Common Bean ◽  
Dna Markers ◽  
Marker Assisted Selection ◽  
Test Method ◽  
White Mold ◽  
Phenotypic Analysis ◽  
Mean Values ◽  
High Heritability ◽  
Bayesian Shrinkage ◽  
The Bayesian Approach

The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F2 population of common bean evaluated by the straw test method. The DNA from 186 F2 plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F2:4 progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F2:4 progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance—13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection.

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