scholarly journals Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge)

2015 ◽  
Vol 14 (4) ◽  
pp. 18302-18314 ◽  
Author(s):  
C. Cheng ◽  
J. Hu ◽  
Y. Zhi ◽  
J.J. Su ◽  
X.K. Zhang ◽  
...  
Keyword(s):  
Small Subunit ◽  
Cyperus Esculentus ◽  
Subunit Gene ◽  
Yellow Nutsedge ◽  
Adp Glucose Pyrophosphorylase

scholarly journals Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivumL.)

2015 ◽  
Vol 10 (2) ◽  
pp. e989033 ◽  
Author(s):  
Shalini Mukherjee ◽  
Claudio Stasolla ◽  
Anita Brûlé-Babel ◽  
Belay T Ayele
Keyword(s):  
Common Wheat ◽  
Gene Promoter ◽  
Small Subunit ◽  
Subunit Gene ◽  
Isolation And Characterization ◽  
Rubisco Small Subunit

scholarly journals A single gene encodes two different transcripts for the ADP-glucose pyrophosphorylase small subunit from barley (Hordeum vulgare)

1996 ◽  
Vol 313 (1) ◽  
pp. 149-154 ◽  
Author(s):  
Tine THORBJØRNSEN ◽  
Per VILLAND ◽  
Leszek A. KLECZKOWSKI ◽  
Odd-Arne OLSEN
Keyword(s):  
Hordeum Vulgare ◽  
Genomic Library ◽  
Single Gene ◽  
Starch Synthesis ◽  
Transit Peptide ◽  
Small Subunit ◽  
Peptide Sequence ◽  
Subunit Gene ◽  
Starchy Endosperm ◽  
Adp Glucose Pyrophosphorylase

ADP-glucose pyrophosphorylase (AGPase), a heterotetrameric enzyme composed of two small and two large subunits, catalyses the first committed step of starch synthesis in plant tissues. In an attempt to learn more about the organization and expression of the small-subunit gene of AGPase, we have studied the small-subunit transcripts as well as the structure of the gene encoding these transcripts in barley (Hordeum vulgare L. cv. Bomi). Two different transcripts (bepsF1 and blps14) were identified: bepsF1 was abundantly expressed in the starchy endosperm but not in leaves, whereas blps14 was isolated from leaves but was also found to be present at a moderate level in the starchy endosperm. The sequences for the two transcripts are identical over approx. 90% of the length, with differences being confined solely to their 5ʹ ends. In blps14, the unique 5ʹ end is 259 nt long and encodes a putative plastid transit peptide sequence. For the 178-nt 5ʹ end of bepsF1, on the other hand, no transit peptide sequence could be recognized. A lambda clone that hybridized to the AGPase transcripts was isolated from a barley genomic library and characterized. The restriction map has suggested a complex organization of the gene, with alternative exons encoding the different 5ʹ ends of the two transcripts followed by nine exons coding for the common part of the transcripts. The sequence of a portion of the genomic clone, covering the alternative 5ʹ-end exons as well as upstream regions, has verified that both transcripts are encoded by the gene. The results suggest that the small-subunit gene of barley AGPase transcribes two different mRNAs by a mechanism classified as alternative splicing.

scholarly journals Isolation and characterisation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS1) promoter in tomato (Solanum lycopersicum L.)

2013 ◽  
Vol 30 (3) ◽  
pp. 279-286 ◽  
Author(s):  
Yukihisa Goto ◽  
Satoko Nonaka ◽  
Yong-Gen Yin ◽  
Teruyuki Koiwa ◽  
Erika Asamizu ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Small Subunit ◽  
Subunit Gene ◽  
Solanum Lycopersicum L ◽  
Adp Glucose Pyrophosphorylase

Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives

Biologia ◽  
2017 ◽  
Vol 72 (12) ◽  
Author(s):  
Xiao-Wei Zhang ◽  
Qing Wang ◽  
Ling-Ling Zhang ◽  
Xiao-Juan Zhong ◽  
Qian-Tao Jiang ◽  
...  
Keyword(s):  
Cdna Sequence ◽  
Small Subunit ◽  
Full Length ◽  
Gene Encoding ◽  
Full Length Cdna ◽  
S Gene ◽  
Limiting Step ◽  
Adp Glucose Pyrophosphorylase

AbstractADP-glucose pyrophosphorylase (AGP) consists of two large (AGP-L) and two small (AGP-S) subunits; it regulates the limiting step in the biosynthesis of starch. Here, we isolated the full-length cDNA sequence of the AGP-S gene (designed as

Isolation and characterization of a higher plant ADP-glucose pyrophosphorylase small subunit homotetramer

FEBS Letters ◽  
2000 ◽  
Vol 482 (1-2) ◽  
pp. 113-118 ◽  
Author(s):  
Peter R. Salamone ◽  
Thomas W. Greene ◽  
Ibrahim H. Kavakli ◽  
Thomas W. Okita
Keyword(s):  
Small Subunit ◽  
Higher Plant ◽  
Isolation And Characterization ◽  
Adp Glucose Pyrophosphorylase

Theileria parva ribosomal internal transcribed spacer sequences exhibit extensive polymorphism and mosaic evolution: application to the characterization of parasites from cattle and buffalo

Parasitology ◽  
1999 ◽  
Vol 118 (6) ◽  
pp. 541-551 ◽  
Author(s):  
N. E. COLLINS ◽  
B. A. ALLSOPP
Keyword(s):  
Genetic Recombination ◽  
Large Subunit ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Rrna Genes ◽  
Gene Pools ◽  
Theileria Parva ◽  
Causative Agents ◽  
Internal Transcribed Spacer Sequences

We sequenced the rRNA genes and internal transcribed spacers (ITS) of several Theileria parva isolates in an attempt to distinguish between the causative agents of East coast fever and Corridor disease. The small subunit (SSU) and large subunit (LSU) rRNA genes from a cloned T. p. lawrencei parasite were sequenced; the former was identical to that of T. p. parva Muguga, and there were minor heterogeneities in the latter. The 5·8S gene sequences of 11 T. parva isolates were identical, but major differences were found in the ITS. Six characterization oligonucleotides were designed to hybridize within the variable ITS1 region; 93·5% of T. p. parva isolates examined were detected by probe TPP1 and 81·8% of T. p. lawrencei isolates were detected by TPL2 and/or TPL3a. There was no absolute distinction between T. p. parva and T. p. lawrencei and the former hybridized with fewer of the probes than did the latter. It therefore seems that a relatively homogenous subpopulation of T. parva has been selected in cattle from a more diverse gene pool in buffalo. The ITSs of both T. p. parva and T. p. lawrencei contained different combinations of identifiable sequence segments, resulting in a mosaic of segments in any one isolate, suggesting that the two populations undergo genetic recombination and that their gene pools are not completely separate.

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