Effect of montelukast on the expression of interleukin-18, telomerase reverse transcriptase, and Bcl-2 in the brain tissue of neonatal rats with hypoxic-ischemic brain damage

2015 ◽  
Vol 14 (3) ◽  
pp. 8901-8908 ◽  
Author(s):  
J.L. Liu ◽  
X.H. Zhao ◽  
D.L. Zhang ◽  
J.B. Zhang ◽  
Z.H. Liu
Keyword(s):  
Reverse Transcriptase ◽  
Brain Tissue ◽  
Brain Damage ◽  
Telomerase Reverse Transcriptase ◽  
Neonatal Rats ◽  
Interleukin 18 ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
The Brain

Significant Role of microRNA-182-5p in Neonatal Hypoxic-Ischemic Injury and Related Mechanism

2020 ◽  
Vol 10 (5) ◽  
pp. 654-661
Author(s):  
Qin Wang ◽  
Ting Wang
Keyword(s):  
Cell Viability ◽  
Brain Tissue ◽  
Brain Damage ◽  
Rat Model ◽  
Target Gene ◽  
Luciferase Reporter ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Qrt Pcr ◽  
The Brain

The purpose of current study was to explore the role and mechanism of microRNA-182-5p (miR182-5p) in neonatal hypoxic ischemic brain damage (HIBD). First, we established a hypoxic-ischemic (HI) rat model and assessed the neurological function of the rats using the Zea Longa score. Then, the level of miR-182-5p in brain tissue of neonatal rats was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Findings revealed that miR-182-5p was significantly down-regulated in the brain tissue of HI rat model. Next, we studied the target gene of miR-182-5p by using TargetScan and dual luciferase reporter assay. Results showed that CASP2 was a direct target gene of miR-182-5p, and the level of CASP2 was significantly up-regulated in the brain tissue of HI rat model. Immediately thereafter, we established an oxygen and glucose deprivation (OGD) cell model of primary cortical neurons, and demonstrated the changes of miR182-5p in cells treated with OGD by qRT-PCR. Finally, to determine the function of miR-182-5p in OGD subjected neuronal cells, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and flow cytometry (FCM) assays were used to study cell viability and apoptosis. The study found that compared with the OGD group, miR-182-5p mimic significantly increased nerve cell viability, reduced cell apoptosis and decreased cleaved-Caspase3/7/8 protein expression, however, all these changes were significantly reversed by overexpression of the CASP2 gene. Taken together, miR-182-5p might protect the nerve cells from ischemia and hypoxia by targeting CASP2, thereby playing a protective role in hypoxic ischemic encephalopathy, which might be a new effective target for neonatal hypoxic ischemic brain damage treatment.

scholarly journals Intraventricular Injection of Human Dental Pulp Stem Cells Improves Hypoxic-Ischemic Brain Damage in Neonatal Rats

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66748 ◽  
Author(s):  
Cheng-zhi Fang ◽  
Yu-jia Yang ◽  
Qin-hong Wang ◽  
Yue Yao ◽  
Xiao-ying Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Brain Damage ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Intraventricular Injection ◽  
Neonatal Rats ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Human Dental Pulp

Progesterone inhibits the expression of cycloxygenase-2 and interleukin-1β in neonatal rats with hypoxic ischemic brain damage

2013 ◽  
Vol 124 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Xiaoyin Wang ◽  
Junhe Zhang ◽  
Daowen Si ◽  
Ruling Shi ◽  
Weihua Dong ◽  
...  
Keyword(s):  
Brain Damage ◽  
Interleukin 1Β ◽  
Neonatal Rats ◽  
Ischemic Brain Damage ◽  
Ischemic Brain

Zonisamide reduces hypoxic-ischemic brain damage in neonatal rats irrespective of its anticonvulsive effect

1994 ◽  
Vol 257 (1-2) ◽  
pp. 131-136 ◽  
Author(s):  
Takahiro Hayakawa ◽  
Yoshihisa Higuchi ◽  
Hiroyuki Nigami ◽  
Haruo Hattori
Keyword(s):  
Brain Damage ◽  
Neonatal Rats ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Anticonvulsive Effect

Antisense in vivo knockdown of synaptotagmin I by HVJ–liposome mediated gene transfer attenuates ischemic brain damage in neonatal rats

2008 ◽  
Vol 30 (5) ◽  
pp. 313-320 ◽  
Author(s):  
Tadaki Omae ◽  
Hiroshi Yoshioka ◽  
Taro Tanaka ◽  
Hideyuki Nagai ◽  
Makoto Saji ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Brain Damage ◽  
Neonatal Rats ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Synaptotagmin I

Studies on cerebral protection of digoxin against hypoxic–ischemic brain damage in neonatal rats

Neuroreport ◽  
2016 ◽  
Vol 27 (12) ◽  
pp. 906-915 ◽  
Author(s):  
Kaiwei Peng ◽  
Danfeng Tan ◽  
Miao He ◽  
Dandan Guo ◽  
Juan Huang ◽  
...  
Keyword(s):  
Brain Damage ◽  
Cerebral Protection ◽  
Neonatal Rats ◽  
Ischemic Brain Damage ◽  
Ischemic Brain

scholarly journals Characteristics of nervous tissue after modeling of focal cerebral ischemia in rats at different periods of reperfusion

2018 ◽  
Vol 24 (3) ◽  
pp. 58-64
Author(s):  
O.I. Savchuk ◽  
G.G. Skibo
Keyword(s):  
Cerebral Ischemia ◽  
Brain Damage ◽  
Nervous Tissue ◽  
Focal Cerebral Ischemia ◽  
Tactile Sensitivity ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Ischemic Period ◽  
The Brain ◽  
Occlusion Period

The stroke-causing problems are extremely important in Ukraine. This makes a heavy burden not only on the health care system, but also on the whole society as a whole. That's why we've studied structural and ultrastructural changes of cortical neurons and striatum of the brain and the development of delayed death of nerve cells after the modeling of the middle cerebral artery occlusion (MCAO) and post ischemic period in rats. We've analyzed the data at different terms after modeling of MCAO. The purpose of the study was to investigate the changes in the nervous tissue in the modeling of focal cerebral ischemia by monofilament occlusion of MCAO in rats at different periods of reperfusion. The statistical processing of primary digital experimental data was carried out using the software Statistica 6.0. It was confirmed that the 60-minute occlusion of the MCAO is an adequate model of focal ischemic brain damage in rats. Changes of locomotor activity and a tactile sensitivity were determined in rats after occlusion and after reperfusion during the post-period period. It was found that in the experimental group with a reperfusion period of 72 hours, a clear increase of the volume of the ischemic area of the brain, accompanied by significant neurological deficiency, was observed. Reduced research activity of the rats was revealed, which was shown in the decrease of the number of squares they crossed, the number of racks, the increase of acts of grooming and the duration of acts of frizings. Following ischemic brain damage, there was also a disbalance of somato-sensory functions, as evidenced by an increase in the time during which the animal took a test stimulus ("Sticky tape") from both the anterior paws when tested for tactile sensitivity (adhesive removal test). An electron microscopic study of the cortex showed that dark wrinkled neurons and enlightened swollen neurons were observed at 72 hours of post-occlusion period, indicating different ways of death of these cells. Changes in striatum were similar to changes in the cortex, which progressed with an increase in the post-occlusion period. The protocol of the serial evaluation of neurological disorders used after MCAO modeling allowed detecting long-term stable functional disorders in laboratory rats. The obtained data indicate significant changes in the structure of the cortex and striatum in the post-ischemic period and the progressive nature of these changes.

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