scholarly journals Analysis of alterations to the transcriptome of Loquat (Eriobotrya japonica Lindl.) under low temperature stress via de novo sequencing

2015 ◽  
Vol 14 (3) ◽  
pp. 9423-9436 ◽  
Author(s):  
R.G. Gong ◽  
J. Lai ◽  
W. Yang ◽  
M.A. Liao ◽  
Z.H. Wang ◽  
...  
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
De Novo ◽  
De Novo Sequencing ◽  
Low Temperature Stress ◽  
Eriobotrya Japonica

scholarly journals Comparative Transcriptome Analysis of Temperature-Induced Green Discoloration in Garlic

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Ningyang Li ◽  
Zhichang Qiu ◽  
Xiaoming Lu ◽  
Bingchao Shi ◽  
Xiudong Sun ◽  
...  
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
De Novo ◽  
Aminolevulinic Acid ◽  
Average Length ◽  
Transcriptome Profiling ◽  
Temperature Treatment ◽  
Low Temperature Stress ◽  
Large Numbers ◽  
Encoding Genes

Green discoloration is one of the most important problems that cause low quality of product in the processing of garlic, which can be induced by low-temperature stress. But the mechanism of low temperature-induced green discoloration is poorly understood. In the present study, the control garlic and three low temperature-treated garlic samples (stored at 4°C with 10, 15, and 40 days, respectively) were used for genome-wide transcriptome profiling analysis. A total of 49280 garlic unigenes with an average length of 1337 bp were de novo assembled, 20231 of which were achieved for functional annotation. When being suffered from 10, 15, and 40 days of low-temperature treatment, an increased degree of discoloration was observed, and a total of 4757, 4401, and 2034 unigenes showed a differential expression, respectively. Finally, 5923 differentially expressed genes (DEGs) were found to respond to the low-temperature stress, of which 3921 were identified in at least two treatments. Among these stress-responsive unigenes, there were large numbers of enzyme-encoding genes, which significantly enriched the pathway “proteasome,” many genes of which are potentially involved in the garlic discoloration, such as 7 alliinase-encoding genes, 5 γ-glutamyltranspeptidase-encoding genes, and 1 δ-aminolevulinic acid dehydratase-encoding gene. These stress-responsive enzyme-encoding genes are possibly responsible for the low-temperature-induced garlic discoloration. The identification of large numbers of DEGs provides a basis for further elucidating the mechanism of low-temperature-induced green discoloration in garlic.

scholarly journals Putrescine Content Parallels Ammonia and Arginine Metabolism in Developing Flowers of the `Washington' Navel Orange

1991 ◽  
Vol 116 (2) ◽  
pp. 280-285 ◽  
Author(s):  
Oded Sagee ◽  
Carol J. Lovatt
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Flower Development ◽  
Biosynthetic Pathway ◽  
De Novo ◽  
Maximum Activity ◽  
Low Temperature Stress ◽  
Navel Orange ◽  
Flower Opening ◽  
Washington Navel

Maximum leaf NH3-NH4+ content and activity of the de novo arginine biosynthetic pathway occurred during the 1st week after transfer of 5-year-old rooted cuttings of the `Washington' navel orange (Citrus sinensis L. Osbeck) from 8 weeks of low-temperature stress [8-hour days (500 μmol·s-1·m-2) at 15 to 18C/16-hour nights at 10 to 13C]. Both aspects declined in parallel during the subsequent 4 weeks of 12-hour days (500 μmol·s-1·m-2) at 24 C/12-hour nights at 19C, which culminated in maximum bloom. Apical flowers of inflorescences initiated in response to 8 weeks of low-temperature stress exhibited maximum tissue concentrations of NH3-NH4+ and putrescine, and maximum activity of the de novo arginine biosynthetic pathway 1 week after transfer of the trees from the low-temperature induction to the higher temperature (flower buds were 7 × 5 mm, length/width). All three criteria decreased in parallel as flowers developed through Stage V (petal fall). In contrast, spermine concentration increased 7-fold during Stage IV of flower development (flower opening). By Stage V, ovaries contained about equal concentrations of putrescine, spermidine, and spermine. The activity of the de novo tyrosine biosynthetic pathway exhibited a pattern of change independent of flower NH3-NH4+ concentration. Observed changes were not due to increased organ weight or size and persisted when the data were expressed per milligram protein. The results of this study demonstrate that leaves and floral buds undergo parallel changes in N metabolism in response to low-temperature, stress-induced flowering and provide evidence that flower NH3-NH4+ content and putrescine synthesis via argine are metabolically correlated during flower development in C. sinensis.

Effects of low-temperature stress on histone modification in ZF4 cells

2019 ◽  
Vol 26 (2) ◽  
pp. 280
Author(s):  
Penglei JIANG ◽  
Yingdi SHI ◽  
Yanwen HOU ◽  
Bingshe HAN ◽  
Junfang ZHANG
Keyword(s):  
Low Temperature ◽  
Histone Modification ◽  
Temperature Stress ◽  
Low Temperature Stress

Effects of low temperature stress on photosynthesis in potato leaves

2014 ◽  
Vol 39 (1) ◽  
pp. 26-30 ◽  
Author(s):  
Yu-zhi QIN ◽  
Jue CHEN ◽  
Zhen XING ◽  
Chang-zheng HE ◽  
Xing-yao XIONG
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Low Temperature Stress

scholarly journals Zinc Seed Priming Improves Spinach Germination at Low Temperature

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 271
Author(s):  
Muhammad Imran ◽  
Asim Mahmood ◽  
Günter Neumann ◽  
Birte Boelt
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Multispectral Imaging ◽  
Germination Rate ◽  
Cost Effective ◽  
Seed Priming ◽  
Seedling Development ◽  
Low Temperature Stress ◽  
Early Seedling ◽  
Early Seedling Development

Low temperature during germination hinders germination speed and early seedling development. Zn seed priming is a useful and cost-effective tool to improve germination rate and resistance to low temperature stress during germination and early seedling development. Spinach was tested to improve germination and seedling development with Zn seed priming under low temperature stress conditions. Zn priming increased seed Zn concentration up to 48 times. The multispectral imaging technique with VideometerLab was used as a non-destructive method to differentiate unprimed, water- and Zn-primed spinach seeds successfully. Localization of Zn in the seeds was studied using the 1,5-diphenyl thiocarbazone (DTZ) dying technique. Active translocation of primed Zn in the roots of young seedlings was detected with laser confocal microscopy. Zn priming of spinach seeds at 6 mM Zn showed a significant increase in germination rate and total germination under low temperature at 8 °C.

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