scholarly journals Isolation and characterization of CCoAOMT in interspecific hybrid of Acacia auriculiformis x Acacia mangium - a key gene in lignin biosynthesis

2014 ◽  
Vol 13 (3) ◽  
pp. 7217-7238 ◽  
Author(s):  
S.L. Pang ◽  
S.S. Ong ◽  
H.H. Lee ◽  
Z. Zamri ◽  
K.I. Kandasamy ◽  
...  
Keyword(s):  
Interspecific Hybrid ◽  
Acacia Mangium ◽  
Lignin Biosynthesis ◽  
Acacia Auriculiformis ◽  
Isolation And Characterization

scholarly journals A pseudogene of caffeic acid-o-methyltransferase (COMT) in Acacia mangium: Comparative analysis with other COMT plant promoters

2018 ◽  
Author(s):  
Azmah Abdul Latif ◽  
Sheh May Tam ◽  
Wickneswari Ratnam
Keyword(s):  
Stop Codon ◽  
Regulatory Region ◽  
Premature Stop Codon ◽  
Acacia Mangium ◽  
Lignin Biosynthesis ◽  
Regulatory Elements ◽  
Wood Products ◽  
Acacia Auriculiformis ◽  
Myb Transcription Factor ◽  
Stem Loop

ABSTRACTAcacia mangium is a prominent tree species in the forest plantation industry of Southeast Asia, grown mainly to produce pulp and paper, and to a lesser extent wood chips and solid wood products. Lignin, a natural complex polymer used by plants for structural support and defence, has to be chemically removed during the production of quality paper. Delignification is very expensive and moreover, is an environmental pollutant. Understanding the complex mechanisms that underlie the regulation of lignin biosynthetic genes requires in-depth knowledge of not only the genes involved but also their regulatory elements. Using Thermal Asymmetric Interlaced PCR, a 770 bp promoter sequence with 93% identity with COMT1 gene from Acacia auriculiformis × A. mangium hybrid was isolated from A. mangium. Bioinformatics analysis revealed the presence of cis acting elements commonly found in other lignin biosynthesis genes such as TATA box, CAAT box, W box, AC-I and AC-11 elements. However, a nonsense mutation that created a premature stop codon was found on the first exon. Modelling of MYB transcription factor binding site on this newly isolated pseudogene shows it has binding sites for important transcription factors involved in lignin biosynthesis both in Arabidopsis thaliana and Eucalyptus grandis. Given the remarkable structures of its regulatory region, the possible structure of its transcript was detected using Mfold. Results show the transcript are capable of forming stem loop structures, a characteristic commonly attributed to presence of miRNA. Possible functions of pseudoAmCOMT1 were discussed.

scholarly journals Isolation and characterization of flower-specific transcripts in Acacia mangium

2005 ◽  
Vol 25 (2) ◽  
pp. 167-178 ◽  
Author(s):  
X. J. Wang ◽  
X. L. Cao ◽  
Y. Hong
Keyword(s):  
Acacia Mangium ◽  
Isolation And Characterization

Isolation and characterization of a Pinus radiata lignin biosynthesis-related O-methyltransferase promoter

2002 ◽  
Vol 20 (11) ◽  
pp. 1052-1060 ◽  
Author(s):  
R. Moyle ◽  
J. Moody ◽  
L. Phillips ◽  
C. Walter ◽  
A. Wagner
Keyword(s):  
Pinus Radiata ◽  
Lignin Biosynthesis ◽  
Isolation And Characterization

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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