scholarly journals Absolute quantification of free tumor cells in the peripheral blood of gastric cancer patients

2014 ◽  
Vol 13 (2) ◽  
pp. 4425-4432 ◽  
Author(s):  
N. Bayat ◽  
M.M. Mokhtari ◽  
M. Rezaei-Tavirani ◽  
A. Baradaran-rafii ◽  
S. Rahman Zadeh ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Absolute Quantification ◽  
Gastric Cancer Patients

scholarly journals Molecular Detection of Disseminated Tumor Cells in the Peripheral Blood of Patients with Gastric Cancer: Evaluation of Their Prognostic Significance

2006 ◽  
Vol 22 (3) ◽  
pp. 103-109 ◽  
Author(s):  
C. H. Wu ◽  
S. R. Lin ◽  
J. S. Hsieh ◽  
F. M. Chen ◽  
C. Y. Lu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Prognostic Significance ◽  
Disseminated Tumor Cells ◽  
Postoperative Recurrence ◽  
Healthy Individuals ◽  
Gastric Cancer Patients ◽  
Cea Mrna

Early detection of disseminated tumor cells in the peripheral blood of patients with early stage gastric cancer could help to improve the outcome after tumor resection. The aim of this study is to evaluate the prognostic significance of tumor-related mRNA for the detection of circulating tumor cells in gastric cancer patients by a reverse-transcriptase polymerase chain reaction (RT-PCR) method. We simultaneously analyzed human telomerase reverse transcriptase (hTERT), cytokeratin-19 (CK-19), cytokeratin-20 (CK-20) and carcinoembryonic antigen (CEA) mRNA (messenger RNA) expression in the peripheral blood of 42 gastric cancer patients and 30 healthy individuals. Additionally, analyses were carried out for the correlation of these four molecular markers with patients’ clinicopathologic features, as well as the occurrence of postoperative recurrence/metastasis. Among 42 gastric cancer patients, the prevalence of mRNA for hTERT, CK-19, CK-20, and CEA was 61.9% (26/42), 69% (29/42), 61.9% (26/42), and 78.6% (33/42), respectively. All 30 healthy individuals were negative for hTERT and CEA mRNA, while two were positive for either CK-19 mRNA or CK-20 mRNA. Positive CEA mRNA was significantly correlated with tumor size (p= 0.008), vessel invasion (p= 0.001), depth of tumor invasion (p= 0.007), lymph node metastasis (p< 0.001), and TNM stage (p< 0.001). In addition, the multivariate logistic regression demonstrated that CEA mRNA expression was an independent and significant predictor for postoperative recurrence/metastasis (p= 0.032). Our findings suggest that CEA mRNA may be a more reliable marker than hTERT, CK-19 and CK-20 for the detection of circulating cancer cells in gastric cancer patients' peripheral blood. Patients with positive CEA mRNA expression in peripheral blood have a significantly higher risk of postoperative recurrence/metastasis.

The prognostic significance of isolated tumor cells in the lymph nodes of gastric cancer patients

2010 ◽  
Vol 13 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Takeo Fukagawa ◽  
Mitsuru Sasako ◽  
Seiji Ito ◽  
Hayao Nakanishi ◽  
Hisae Iinuma ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Prognostic Significance ◽  
Isolated Tumor Cells ◽  
Gastric Cancer Patients

scholarly journals Detection and HER2 Expression of Circulating Tumor Cells in Advanced Gastric Cancer Patients

2012 ◽  
Vol 23 ◽  
pp. xi118
Author(s):  
S. Matsusaka ◽  
K. Chin ◽  
M. Ogur ◽  
E. Shinozaki ◽  
M. Suenaga ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Advanced Gastric Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Her2 Expression ◽  
Gastric Cancer Patients

Clinical Relevance of Occult Tumor Cells in Lymph Nodes From Gastric Cancer Patients

2005 ◽  
Vol 29 (9) ◽  
pp. 1135-1144 ◽  
Author(s):  
Fania S Doekhie ◽  
Wilma E Mesker ◽  
J Han J. M. van Krieken ◽  
Niels F. M Kok ◽  
Henk H Hartgrink ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Clinical Relevance ◽  
Occult Tumor ◽  
Gastric Cancer Patients ◽  
Occult Tumor Cells
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