scholarly journals Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis

2014 ◽  
Vol 13 (2) ◽  
pp. 4348-4360
Author(s):  
W. Zhai ◽  
Y. Zhao ◽  
L.X. Zhang ◽  
X.J. Li
Keyword(s):  
Phylogenetic Analysis ◽  
Disease Resistance ◽  
Resistance Gene ◽  
Hevea Brasiliensis ◽  
Disease Resistance Gene ◽  
Resistance Gene Candidates

Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in banana

2007 ◽  
Vol 278 (4) ◽  
pp. 443-453 ◽  
Author(s):  
Santy Peraza-Echeverria ◽  
Andrew James-Kay ◽  
Blondy Canto-Canché ◽  
Eduardo Castillo-Castro
Keyword(s):  
Phylogenetic Analysis ◽  
Disease Resistance ◽  
Resistance Gene ◽  
Disease Resistance Gene ◽  
Resistance Gene Candidates

scholarly journals Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing

2019 ◽  
Author(s):  
Huanhuan Li ◽  
Zhenjie Dong ◽  
Chao Ma ◽  
Xiubin Tian ◽  
Zhiguo Xiang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Disease Resistance Gene ◽  
R Genes ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistance Gene Candidates

AbstractPowdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

Cloning and Analysis of NBS-LRR Type Disease Resistance Gene Analogs from Rosa rubus in Yunnan

2012 ◽  
Vol 34 (1) ◽  
pp. 56
Author(s):  
Ling CHEN ◽  
Hao ZHANG ◽  
Xian-Qin QIU ◽  
Hui-Jun YAN ◽  
Qi-Gang WANG ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Disease Resistance Gene ◽  
Resistance Gene Analogs

Organization, Expression and Evolution of a Disease Resistance Gene Cluster in Soybean

Genetics ◽  
2002 ◽  
Vol 162 (4) ◽  
pp. 1961-1977
Author(s):  
Michelle A Graham ◽  
Laura Fredrick Marek ◽  
Randy C Shoemaker
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Developmental Stages ◽  
Gene Evolution ◽  
Pcr Amplification ◽  
Disease Resistance Genes ◽  
Disease Resistance Gene ◽  
R Genes ◽  
Specific Primers

Abstract PCR amplification was previously used to identify a cluster of resistance gene analogues (RGAs) on soybean linkage group J. Resistance to powdery mildew (Rmd-c), Phytophthora stem and root rot (Rps2), and an ineffective nodulation gene (Rj2) map within this cluster. BAC fingerprinting and RGA-specific primers were used to develop a contig of BAC clones spanning this region in cultivar “Williams 82” [rps2, Rmd (adult onset), rj2]. Two cDNAs with homology to the TIR/NBD/LRR family of R-genes have also been mapped to opposite ends of a BAC in the contig Gm_Isb001_091F11 (BAC 91F11). Sequence analyses of BAC 91F11 identified 16 different resistance-like gene (RLG) sequences with homology to the TIR/NBD/LRR family of disease resistance genes. Four of these RLGs represent two potentially novel classes of disease resistance genes: TIR/NBD domains fused inframe to a putative defense-related protein (NtPRp27-like) and TIR domains fused inframe to soybean calmodulin Ca2+-binding domains. RT-PCR analyses using gene-specific primers allowed us to monitor the expression of individual genes in different tissues and developmental stages. Three genes appeared to be constitutively expressed, while three were differentially expressed. Analyses of the R-genes within this BAC suggest that R-gene evolution in soybean is a complex and dynamic process.

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