Expression patterns and promoter activity analysis of UGP2 in pigs

2014 ◽  
Vol 13 (1) ◽  
pp. 1358-1365
Author(s):  
J.Y. Yu ◽  
S.M. Shao ◽  
K. Chen ◽  
M.G. Lei ◽  
Y.Z. Xiong
Keyword(s):  
Promoter Activity ◽  
Expression Patterns ◽  
Activity Analysis ◽  
Promoter Activity Analysis

Characterization of NtREL1, a novel root-specific gene from tobacco, and upstream promoter activity analysis in homologous and heterologous hosts

2016 ◽  
Vol 35 (4) ◽  
pp. 757-769 ◽  
Author(s):  
Chong Zhang ◽  
Shufang Pan ◽  
Hua Chen ◽  
Tiecheng Cai ◽  
Chunhong Zhuang ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Activity Analysis ◽  
Specific Gene ◽  
Upstream Promoter ◽  
Promoter Activity Analysis

Characterization and Promoter Activity Analysis of a New Porcine Gene: NICE-3

2010 ◽  
Vol 29 (3) ◽  
pp. 141-147
Author(s):  
Zongwu Zhang ◽  
Delin Mo ◽  
Fei Ling ◽  
Chong Wang ◽  
Anning Li ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Activity Analysis ◽  
Porcine Gene ◽  
Promoter Activity Analysis

scholarly journals Identification and Promoter Activity Analysis of Porcine miR-181 and miR-1

2012 ◽  
Vol 11 (6) ◽  
pp. 986-992
Author(s):  
Hai-xin ZHANG ◽  
Rui ZHANG ◽  
Yi-nan LIU ◽  
Dao-lin WANG ◽  
Yan-he ZHAO ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Activity Analysis ◽  
Promoter Activity Analysis

Expression characterization and the promoter activity analysis of zebrafish hdac4

2011 ◽  
Vol 38 (2) ◽  
pp. 585-593 ◽  
Author(s):  
Kecheng Zhu ◽  
Huanling Wang ◽  
Yasmeen Gul ◽  
Yuhua Zhao ◽  
Weimin Wang ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Activity Analysis ◽  
Expression Characterization ◽  
Promoter Activity Analysis

scholarly journals The TetR-Type Transcriptional Repressor RolR from Corynebacterium glutamicum Regulates Resorcinol Catabolism by Binding to a Unique Operator,rolO

2012 ◽  
Vol 78 (17) ◽  
pp. 6009-6016 ◽  
Author(s):  
Tang Li ◽  
Kexin Zhao ◽  
Yan Huang ◽  
Defeng Li ◽  
Cheng-Ying Jiang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Promoter Activity ◽  
Activity Analysis ◽  
Inverted Repeats ◽  
Catabolic Pathway ◽  
Transcription Start ◽  
Content Type ◽  
Transcription Start Sites ◽  
Reverse Transcription Pcr ◽  
Promoter Activity Analysis

ABSTRACTTherol(designated forresorcinol) gene clusterrolRHMDis involved in resorcinol catabolism inCorynebacterium glutamicum, and RolR is the TetR-type regulator. In this study, we investigated how RolR regulated the transcription of therolgenes inC. glutamicum. The transcription start sites and promoters ofrolRandrolHMDwere identified. Quantitative reverse transcription-PCR and promoter activity analysis indicated that RolR negatively regulated the transcription ofrolHMDand of its own gene. Further, a 29-bp operatorrolOwas located at the intergenic region ofrolRandrolHMDand was identified as the sole binding site for RolR. It contained two overlapping inverted repeats and they were essential for RolR-binding. The binding of RolR torolOwas affected by resorcinol and hydroxyquinol, which are the starting compounds of resorcinol catabolic pathway. These two compounds were able to dissociate RolR-rolOcomplex, thus releasing RolR from the complex and derepressing the transcription ofrolgenes inC. glutamicum. It is proposed that the binding of RolR to its operatorrolOblocks the transcription ofrolHMDand of its own gene, thus negatively regulated resorcinol degradation inC. glutamicum.

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