scholarly journals Genetic features of a translation initiation system composed of IRES element, nucleotide context surrounding the initiation codon, and translation initiation region of classical swine fever virus RNA

2014 ◽  
Vol 13 (4) ◽  
pp. 10803-10810
Author(s):  
X.-X. Ma ◽  
Y.-P. Feng ◽  
Y.-Q. Zhao ◽  
J.-L. Liu ◽  
L. Chen ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Initiation Codon ◽  
Translation Initiation Region ◽  
Initiation System ◽  
Virus Rna ◽  
Genetic Features ◽  
Nucleotide Context ◽  
Ires Element

scholarly journals The 3′-Terminal Hexamer Sequence of Classical swine fever virus RNA Plays a Role in Negatively Regulating the IRES-Mediated Translation

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33764 ◽  
Author(s):  
Shih-Wei Huang ◽  
Meng-Yu Chan ◽  
Wei-Li Hsu ◽  
Chin-Cheng Huang ◽  
Ching-Hsiu Tsai
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Virus Rna

scholarly journals Common Host Factors Involved in the Function of the Foot-and-mouth Disease Virus and Classical Swine Fever Virus Internal Ribosomal Entry Sites

2021 ◽  
Author(s):  
Yutaro Ide ◽  
Nobumasa Ito ◽  
Takafumi Matsui ◽  
Kyoko Tsukiyama-Kohara
Keyword(s):  
Disease Virus ◽  
Classical Swine Fever Virus ◽  
Foot And Mouth Disease ◽  
Classical Swine Fever ◽  
Host Factor ◽  
Mouth Disease ◽  
Fever Virus ◽  
Host Factors ◽  
Mouth Disease Virus ◽  
Ires Element

Abstract Foot-and-mouth disease virus (FMDV) and classical swine fever virus (CSFV) both possess positive strand RNA genomes and an internal ribosomal entry site (IRES) element within their 5¢-untranslated regions. To identify common host factors involved with the activity of these IRESes, we utilized cell lines expressing a bicistronic luciferase reporter plasmid, which contained an FMDV-IRES or CSFV-IRES element between the Renilla and firefly luciferase genes. First, we treated FMDV-IRES cells with French maritime pine extract, Pycnogenol® (PYC), and evaluated its suppressive effect on FMDV-IRES activity, as anti-viral effect of PYC was reported so far. We next performed microarray analysis to identify host factors affected by PYC, and confirmed host-factor-IRES interaction by applying host factor-specific siRNAs. We found that polycystic kidney disease 1-like 3 (PKD1L3) and ubiquitin specific peptidase 31 (USP31) are involved in FMDV-IRES activity. Moreover, silencing of these factors also significantly suppressed CSFV-IRES activity. Accordingly, we suggest that PKD1L3 and USP31 are host factors that are involved in the function of the FMDV and CSFV-IRES elements.

scholarly journals Modulation of Translation Initiation Efficiency in Classical Swine Fever Virus

2012 ◽  
Vol 86 (16) ◽  
pp. 8681-8692 ◽  
Author(s):  
Martin Barfred Friis ◽  
Thomas Bruun Rasmussen ◽  
Graham J. Belsham
Keyword(s):  
Translation Initiation ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Recombinant Vaccinia Virus ◽  
Fever Virus ◽  
Translation Efficiency ◽  
Entry Site ◽  
T7 Promoter ◽  
Artificial Chromosomes

Modulation of translation initiation efficiency on classical swine fever virus (CSFV) RNA can be achieved by targeted mutations within the internal ribosome entry site (IRES). In this study, cDNAs corresponding to the wild-type (wt) or mutant forms of the IRES of CSFV strain Paderborn were amplified and inserted into dicistronic reporter plasmids encoding Fluc and Rluc under the control of a T7 promoter. The mutations were within domains II, IIId1, and IIIf of the IRES. The plasmids were transfected into baby hamster kidney (BHK) cells infected with recombinant vaccinia virus vTF7-3, which expresses the T7 RNA polymerase. IRES mutants with different levels of IRES activity were identified and then introduced by homologous recombination into bacterial artificial chromosomes (BACs) containing CSFV Paderborn cDNA downstream of a T7 promoter. From the wt and mutant BACs, full-length CSFV RNA transcripts were producedin vitroand electroporated into porcine PK15 cells. Rescued mutant viruses were obtained from RNAs that contained mutations within domain IIIf which retained more than 75% of the wt translation efficiency. Sequencing of cDNA generated from these rescued viruses verified the maintenance of the introduced changes within the IRES. The growth characteristics of each rescued mutant virus were compared to those of the wt virus. It was shown that viable mutant viruses with reduced translation initiation efficiency can be designed and generated and that viruses containing mutations within domain IIIf of the IRES have reduced growth in cell culture compared to the wt virus.

scholarly journals RNA-protein interactions within the internal translation initiation region of encephalomyocarditis virus RNA

1991 ◽  
Vol 19 (18) ◽  
pp. 4999-5005 ◽  
Author(s):  
A.V. Borovjagin ◽  
M.V. Ezrokhi ◽  
V.M. Rostapshov ◽  
T.Yu. Ugarova ◽  
T.F. Bystrova ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Protein Interactions ◽  
Encephalomyocarditis Virus ◽  
Translation Initiation Region ◽  
Virus Rna ◽  
Internal Translation
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