scholarly journals Chromosome mapping of 18S rDNA and 5S rDNA by dual-color fluorescence in situ hybridization in the half-smooth tongue sole (Cynoglossus semilaevis)

2014 ◽  
Vol 13 (4) ◽  
pp. 10761-10768 ◽  
Author(s):  
L. Jiang ◽  
J. Jiang ◽  
J. Liu ◽  
J. Yuan ◽  
Y. Chen ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
18S Rdna ◽  
5S Rdna ◽  
Chromosome Mapping ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole ◽  
Dual Color ◽  
Half Smooth Tongue Sole

scholarly journals Fluorescence In Situ Hybridization (FISH) Analysis of the Locations of the Oligonucleotides 5S rDNA, (AGGGTTT)3, and (TTG)6 in Three Genera of Oleaceae and Their Phylogenetic Framework

Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 375 ◽  
Author(s):  
Xiaomei Luo ◽  
Juncheng Liu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
5S Rdna ◽  
Fish Analysis ◽  
Cytogenetic Map ◽  
Ligustrum Lucidum ◽  
Starting Point ◽  
Central Regions ◽  
Almost All

We report the cytogenetic map for a collection of species in the Oleaceae, and test similarities among the karyotypes relative to their known species phylogeny. The oligonucleotides 5S ribosomal DNA (rDNA), (AGGGTTT)3, and (TTG)6 were used as fluorescence in situ hybridization (FISH) probes to locate the corresponding chromosomes in three Oleaceae genera: Fraxinus pennsylvanica, Syringa oblata, Ligustrum lucidum, and Ligustrum × vicaryi. Forty-six small chromosomes were identified in four species. (AGGGTTT)3 signals were observed on almost all chromosome ends of four species, but (AGGGTTT)3 played no role in distinguishing the chromosomes but displayed intact chromosomes and could thus be used as a guide for finding chromosome counts. (TTG)6 and 5S rDNA signals discerned several chromosomes located at subterminal or central regions. Based on the similarity of the signal pattern (mainly in number and location and less in intensity) of the four species, the variations in the 5S rDNA and (TTG)6 distribution can be ordered as L. lucidum < L. × vicaryi < F. pennsylvanica < S. oblata. Variations have observed in the three genera. The molecular cytogenetic data presented here might serve as a starting point for further larger-scale elucidation of the structure of the Oleaceae genome, and comparison with the known phylogeny of Oleaceae family.

Variation in highly repetitive DNA composition of heterochromatin in rye studied by fluorescence in situ hybridization

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1061-1069 ◽  
Author(s):  
A. Cuadrado ◽  
N. Jouve ◽  
C. Ceoloni
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Individual Identification ◽  
Highly Repetitive Dna ◽  
The Individual

The molecular characterization of heterochromatin in six lines of rye has been performed using fluorescence in situ hybridization (FISH). The highly repetitive rye DNA sequences pSc 119.2, pSc74, and pSc34, and the probes pTa71 and pSc794 containing the 25S–5.8S–18S rDNA (NOR) and the 5S rDNA multigene families, respectively, were used. This allowed the individual identification of all seven rye chromosomes and most chromosome arms in all lines. All varieties showed similar but not identical patterns. A standard in situ hybridization map was constructed following the nomenclature system recommended for C-bands. All FISH sites observed appeared to correspond well with C-band locations, but not all C-banding sites coincided with hybridization sites of the repetitive DNA probes used. Quantitative and qualitative differences between different varieties were found for in situ hybridization response at corresponding sites. Variation between plants and even between homologous chromosomes of the same plant was found in open-pollinated lines. In inbred lines, the in situ pattern of the homologues was practically identical and no variation between plants was detected. The observed quantitative and qualitative differences are consistent with a corresponding variation for C-bands detected both within and between cultivars.Key words: fluorescence in situ hybridization, repetitive DNA, rye, Secale cereale, polymorphism.

Detection of t(14;18) in follicular lymphoma by dual-color fluorescence in situ hybridization on paraffin-embedded tissue sections

2004 ◽  
Vol 150 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Yosuke Matsumoto ◽  
Kenichi Nomura ◽  
Sawako Matsumoto ◽  
Kyoji Ueda ◽  
Mitsushige Nakao ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Follicular Lymphoma ◽  
Fluorescence In Situ Hybridization ◽  
Tissue Sections ◽  
Dual Color

Evaluating sex chromosome content of sorted human sperm samples with use of dual-color fluorescence in situ hybridization

1997 ◽  
Vol 176 (6) ◽  
pp. 1172-1180 ◽  
Author(s):  
W.Edward Richards ◽  
Sheila M. Dobin ◽  
Vicki Malone ◽  
Alfred B. Knight ◽  
Thomas J. Kuehl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosome ◽  
Human Sperm ◽  
Dual Color

Chromosome mapping of the human cytidine-5?-triphosphate synthetase (CTPS) gene to band 1p34.1?p34.3 by fluorescence in situ hybridization

1991 ◽  
Vol 88 (1) ◽  
pp. 119-121 ◽  
Author(s):  
Ei-ichi Takahashi ◽  
Masatake Yamauchi ◽  
Hideo Tsuji ◽  
Akitsu Hitomi ◽  
Mark Meuth ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Mapping

Yeast artificial chromosome mapping of the cystinosis locus on chromosome 17p by fluorescence in situ hybridization

1996 ◽  
Vol 98 (3) ◽  
pp. 321-322 ◽  
Author(s):  
Ingrid Stec ◽  
Ulrike Peters ◽  
Erik Harms ◽  
Michael R. Koehler ◽  
M. Schmid ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Chromosome Mapping ◽  
Artificial Chromosome

scholarly journals Dual-Color Fluorescence In Situ Hybridization Reveals an Association of Chromosome 8q22 but Not 8p21 Imbalance with High Grade Invasive Breast Carcinoma

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e70790 ◽  
Author(s):  
Logan C. Walker ◽  
Margaret McDonald ◽  
J. Elisabeth Wells ◽  
Gavin C. Harris ◽  
Bridget A. Robinson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Breast Carcinoma ◽  
Fluorescence In Situ Hybridization ◽  
Invasive Breast Carcinoma ◽  
High Grade ◽  
Dual Color

Detection of genetic alterations in primary bladder carcinoma with dual-color and multiplex fluorescence in situ hybridization

2004 ◽  
Vol 149 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Maria I. Stamouli ◽  
Anna D. Panani ◽  
Angeliki D. Ferti ◽  
Constantina Petraki ◽  
R.T.D. Oliver ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Bladder Carcinoma ◽  
Genetic Alterations ◽  
Dual Color ◽  
Primary Bladder
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