scholarly journals Phylogenetic analyses of the hemagglutinin gene of wild-type strains of canine distemper virus in southern Brazil

2013 ◽  
Vol 12 (3) ◽  
pp. 2549-2555 ◽  
Author(s):  
F.J. Negrão ◽  
N.R. Gardinali ◽  
S.A. Headley ◽  
A.A. Alfieri ◽  
M.A. Fernandez ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Phylogenetic Analyses ◽  
Southern Brazil ◽  
Canine Distemper ◽  
Wild Type ◽  
Hemagglutinin Gene ◽  
Type Strains

scholarly journals Molecular detection and phylogenetic relationship of wild-type strains of canine distemper virus in symptomatic dogs from Uberlândia, Minas Gerais

2015 ◽  
Vol 67 (6) ◽  
pp. 1510-1518
Author(s):  
S.A. Headley ◽  
T.R. Santos ◽  
L. Bodnar ◽  
J.P.E. Saut ◽  
A.P. Silva ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Phylogenetic Analyses ◽  
Clinical Manifestations ◽  
N Gene ◽  
Clinical Samples ◽  
Canine Distemper ◽  
Wild Type ◽  
Age Related ◽  
Relationship Of ◽  
Type Strains

This study investigated the occurrence of canine distemper virus (CDV) by evaluating the presence of viral RNA within urine samples of dogs from Uberlândia, MG, with clinical manifestations suggestive of infection by CDV by targeting the CDV N gene. Of the clinical samples collected ( n =33), CDV viruria was detected in 45.5%. Five dogs died spontaneously; all had characteristic CDV-associated histopathological alterations and demonstrated CDV viruria. Statistical analyses revealed that the age, gender, breed, or the organ system of the dog affected had no influence on the occurrence of canine distemper. Myoclonus and motor incoordination were the most significant neurological manifestations observed. A direct association was observed between keratoconjunctivitis and dogs with CDV viruria. These findings suggest that CDV viruria in symptomatic dogs might not be age related, and that symptomatic dogs can demonstrate clinical manifestations attributed to CDV without viruria identified by RT-PCR. Additionally, the results of the sequence identities analysed have suggested that all Brazilian wild-type strains of CDV currently identified are closely related and probably originated from the same lineage of CDV. Nevertheless, phylogenetic analyses suggest that there are different clusters of wild-type strains of CDV circulating within urban canine populations in Brazil.

scholarly journals Disease Duration Determines Canine Distemper Virus Neurovirulence

2007 ◽  
Vol 81 (21) ◽  
pp. 12066-12070 ◽  
Author(s):  
François Bonami ◽  
Penny A. Rudd ◽  
Veronika von Messling
Keyword(s):  
Canine Distemper Virus ◽  
Disease Duration ◽  
Subsequent Development ◽  
Neurological Involvement ◽  
Disease Course ◽  
Canine Distemper ◽  
Wild Type ◽  
Neurological Signs ◽  
H Protein ◽  
Type Strains

ABSTRACT The Morbillivirus hemagglutinin (H) protein mediates attachment to the target cell. To evaluate its contribution to canine distemper virus neurovirulence, we exchanged the H proteins of the wild-type strains 5804P and A75 and assessed the pathogenesis of the chimeric viruses in ferrets. Both strains are lethal to ferrets; however, 5804P causes a 2-week disease without neurological signs, whereas A75 is associated with a longer disease course and neurological involvement. We observed that both H proteins supported neuroinvasion and the subsequent development of clinical neurological signs if given enough time, demonstrating that disease duration is the main neurovirulence determinant.

scholarly journals Genome Sequences of Three Vaccine Strains and Two Wild-Type Canine Distemper Virus Strains from a Recent Disease Outbreak in South Africa

2017 ◽  
Vol 5 (27) ◽  
Author(s):  
Angelika K. Loots ◽  
Morné Du Plessis ◽  
Desiré Lee Dalton ◽  
Emily Mitchell ◽  
Estelle H. Venter
Keyword(s):  
South Africa ◽  
South African ◽  
Canine Distemper Virus ◽  
Disease Outbreak ◽  
Canine Distemper ◽  
Wild Type ◽  
Genome Sequences ◽  
African Lineage ◽  
Type Strains ◽  
Virus Strains

ABSTRACT Canine distemper virus causes global multihost infectious disease. This report details complete genome sequences of three vaccine and two new wild-type strains. The wild-type strains belong to the South African lineage, and all three vaccine strains to the America 1 lineage. This constitutes the first genomic sequences of this virus from South Africa.

scholarly journals Identification of Amino Acid Substitutions with Compensational Effects in the Attachment Protein of Canine Distemper Virus

2014 ◽  
Vol 88 (14) ◽  
pp. 8057-8064 ◽  
Author(s):  
Ursula Sattler ◽  
Mojtaba Khosravi ◽  
Mislay Avila ◽  
Paola Pilo ◽  
Johannes P. Langedijk ◽  
...  
Keyword(s):  
Amino Acid ◽  
Single Nucleotide Polymorphisms ◽  
Canine Distemper Virus ◽  
Nucleotide Polymorphisms ◽  
Canine Distemper ◽  
Wild Type ◽  
Attachment Protein ◽  
Single Nucleotide ◽  
Hemagglutinin Gene ◽  
H Gene

ABSTRACTThe hemagglutinin (H) gene of canine distemper virus (CDV) encodes the receptor-binding protein. This protein, together with the fusion (F) protein, is pivotal for infectivity since it contributes to the fusion of the viral envelope with the host cell membrane. Of the two receptors currently known for CDV (nectin-4 and the signaling lymphocyte activation molecule [SLAM]), SLAM is considered the most relevant for host susceptibility. To investigate how evolution might have impacted the host-CDV interaction, we examined the functional properties of a series of missense single nucleotide polymorphisms (SNPs) naturally accumulating within the H-gene sequences during the transition between two distinct but related strains. The two strains, a wild-type strain and a consensus strain, were part of a single continental outbreak in European wildlife and occurred in distinct geographical areas 2 years apart. The deduced amino acid sequence of the two H genes differed at 5 residues. A panel of mutants carrying all the combinations of the SNPs was obtained by site-directed mutagenesis. The selected mutant, wild type, and consensus H proteins were functionally evaluated according to their surface expression, SLAM binding, fusion protein interaction, and cell fusion efficiencies. The results highlight that the most detrimental functional effects are associated with specific sets of SNPs. Strikingly, an efficient compensational system driven by additional SNPs appears to come into play, virtually neutralizing the negative functional effects. This system seems to contribute to the maintenance of the tightly regulated function of the H-gene-encoded attachment protein.IMPORTANCETo investigate how evolution might have impacted the host-canine distemper virus (CDV) interaction, we examined the functional properties of naturally occurring single nucleotide polymorphisms (SNPs) in the hemagglutinin gene of two related but distinct strains of CDV. The hemagglutinin gene encodes the attachment protein, which is pivotal for infection. Our results show that few SNPs have a relevant detrimental impact and they generally appear in specific combinations (molecular signatures). These drastic negative changes are neutralized by compensatory mutations, which contribute to maintenance of an overall constant bioactivity of the attachment protein. This compensational mechanism might reflect the reaction of the CDV machinery to the changes occurring in the virus following antigenic variations critical for virulence.

Isolation and Phylogenetic Analysis of the Canine Distemper Virus from a Naturally Infected Dog in China

2020 ◽  
Author(s):  
Hongliang Zhang ◽  
Peipei Meng ◽  
Xiaoming Song ◽  
Shuguang Li ◽  
Ruimei Yang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Canine Distemper Virus ◽  
Glycosylation Site ◽  
High Morbidity ◽  
Canine Distemper ◽  
Wild Type ◽  
Neutralization Titer ◽  
Genotype I ◽  
H Gene ◽  
Type Strains

Background: Canine distemper virus (CDV) can infect a variety of predators, such as Canidae, Mustelidae and Procyonidae family and cause high morbidity and mortality. Genetic/antigenic heterogeneity has been observed among various CDV strains, notably in the haemagglutinin (H) gene. Based on sequence analysis of the H gene, wild-type CDV strains can be divided into distinct genotypes. Methods: We used Vero/SLAM cells to isolate CDV strains from clinically naturally infected dog tissues. Here, the H gene sequence of the isolated strain was analyzed for the restriction enzyme polymorphism with RT-PCR-RFLP method and the method could be used to distinguish CDV vaccine strains from wild-type strains. Furthermore, the genetic and antigenic variation of the H gene was also analyzed.Result: Our results indicated that Vero cells expressing canine SLAM allow the efficient isolation of CDV wild-type strains. The isolated CDV was named Shandong strain. Phylogenetic analysis identified that Shandong strain was CDV wild-type strain, the Asian genotype I. Analysis of the potential glycosylation site of the H gene and determination of the neutralization titer PD50 showed that there were immunogenic differences between the Shandong strain and the vaccine strains.

scholarly journals Sequence Analysis and Expression of the Attachment and Fusion Proteins of Canine Distemper Virus Wild-Type Strain A75/17

1999 ◽  
Vol 73 (3) ◽  
pp. 2263-2269 ◽  
Author(s):  
Pascal Cherpillod ◽  
Karin Beck ◽  
Andreas Zurbriggen ◽  
Riccardo Wittek
Keyword(s):  
Amino Acid ◽  
Fusion Protein ◽  
Translation Initiation ◽  
Fusion Proteins ◽  
Canine Distemper Virus ◽  
Protein A ◽  
Biological Properties ◽  
Canine Distemper ◽  
Wild Type ◽  
Attachment Protein

ABSTRACT The biological properties of wild-type A75/17 and cell culture-adapted Onderstepoort canine distemper virus differ markedly. To learn more about the molecular basis for these differences, we have isolated and sequenced the protein-coding regions of the attachment and fusion proteins of wild-type canine distemper virus strain A75/17. In the attachment protein, a total of 57 amino acid differences were observed between the Onderstepoort strain and strain A75/17, and these were distributed evenly over the entire protein. Interestingly, the attachment protein of strain A75/17 contained an extension of three amino acids at the C terminus. Expression studies showed that the attachment protein of strain A75/17 had a higher apparent molecular mass than the attachment protein of the Onderstepoort strain, in both the presence and absence of tunicamycin. In the fusion protein, 60 amino acid differences were observed between the two strains, of which 44 were clustered in the much smaller F2 portion of the molecule. Significantly, the AUG that has been proposed as a translation initiation codon in the Onderstepoort strain is an AUA codon in strain A75/17. Detailed mutation analyses showed that both the first and second AUGs of strain A75/17 are the major translation initiation sites of the fusion protein. Similar analyses demonstrated that, also in the Onderstepoort strain, the first two AUGs are the translation initiation codons which contribute most to the generation of precursor molecules yielding the mature form of the fusion protein.

scholarly journals Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China

2011 ◽  
Vol 8 (1) ◽  
pp. 520 ◽  
Author(s):  
Bin Tan ◽  
Yong-Jun Wen ◽  
Feng-Xue Wang ◽  
Shu-Qin Zhang ◽  
Xiu-Dong Wang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Canine Distemper Virus ◽  
Phylogenetic Analyses ◽  
Domestic Dogs ◽  
Canine Distemper ◽  
Canine Distemper Virus Strain
Sign in / Sign up

Export Citation Format

Share Document