scholarly journals Methodology Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

2013 ◽  
Vol 12 (1) ◽  
pp. 282-292 ◽  
Author(s):  
B. Canto-Canché ◽  
M. Tzec-Simá ◽  
J.I. Vázquez-Loría ◽  
H. Espadas-Álvarez ◽  
B.H. Chí-Manzanero ◽  
...  
2018 ◽  
Vol 24 (2) ◽  
pp. 77-86
Author(s):  
Sri Wening ◽  
Agus Eko Prasetyo ◽  
Tjut Ahmad Perdana Rozziansha ◽  
Agus Susanto

African pollination weevil (Elaeidobius kamerunicus Faust) has an important role in the productivity of Indonesian oil palm plantation. Up to now, there has not been a comprehensive biological study of the species at molecular level. The basic knowledge is very useful for exploitation of the weevil for effective oil palm fruit set development. This research aimed to obtain DNA extraction protocol of E. kamerunicus for DNA fingerprinting of the species. Results showed that using a DNA extraction kit,material disruption by using micro pestle resulted the highest quantity of DNA, while there were no significant differences of resulted DNA quantity among treatments using tissue lyser for material disruption. DNA extracted by using micro pestle or tissue lyser for material disruption is adequate for DNA fingerprinting using AFLP (Amplified Fragment Length Polymorphism) and sequencing techniques.


Fuels ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 168-178
Author(s):  
Marzia Quaglio ◽  
Daniyal Ahmed ◽  
Giulia Massaglia ◽  
Adriano Sacco ◽  
Valentina Margaria ◽  
...  

Sediment microbial fuel cells (SMFCs) are energy harvesting devices where the anode is buried inside marine sediment, while the cathode stays in an aerobic environment on the surface of the water. To apply this SCMFC as a power source, it is crucial to have an efficient power management system, leading to development of an effective energy harvesting technique suitable for such biological devices. In this work, we demonstrate an effective method to improve power extraction with SMFCs based on anodes alternation. We have altered the setup of a traditional SMFC to include two anodes working with the same cathode. This setup is compared with a traditional setup (control) and a setup that undergoes intermittent energy harvesting, establishing the improvement of energy collection using the anodes alternation technique. Control SMFC produced an average power density of 6.3 mW/m2 and SMFC operating intermittently produced 8.1 mW/m2. On the other hand, SMFC operating using the anodes alternation technique produced an average power density of 23.5 mW/m2. These results indicate the utility of the proposed anodes alternation method over both the control and intermittent energy harvesting techniques. The Anode Alternation can also be viewed as an advancement of the intermittent energy harvesting method.


2021 ◽  
Vol 775 ◽  
pp. 145904
Author(s):  
Jaecheul Yu ◽  
Younghyun Park ◽  
Evy Widyaningsih ◽  
Sunah Kim ◽  
Younggy Kim ◽  
...  

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