scholarly journals Genetic variations in the active efflux pump genes acrA/B and tolC in different drug-induced strains of Escherichia coli CVCC 1547

2013 ◽  
Vol 12 (3) ◽  
pp. 2829-2836 ◽  
Author(s):  
J.H. Liu ◽  
Y.S. Pan ◽  
L. Yuan ◽  
H. Wu ◽  
G.Z. Hu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Efflux Pump ◽  
Genetic Variations ◽  
Drug Induced ◽  
Active Efflux ◽  
Active Efflux Pump

scholarly journals MexAB-OprM Efflux Pump Interaction with the Peptidoglycan of Escherichia coli and Pseudomonas aeruginosa

2021 ◽  
Vol 22 (10) ◽  
pp. 5328
Author(s):  
Miao Ma ◽  
Margaux Lustig ◽  
Michèle Salem ◽  
Dominique Mengin-Lecreulx ◽  
Gilles Phan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Cell Division ◽  
Membrane Proteins ◽  
Outer Membrane ◽  
Efflux Pump ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Active Efflux

One of the major families of membrane proteins found in prokaryote genome corresponds to the transporters. Among them, the resistance-nodulation-cell division (RND) transporters are highly studied, as being responsible for one of the most problematic mechanisms used by bacteria to resist to antibiotics, i.e., the active efflux of drugs. In Gram-negative bacteria, these proteins are inserted in the inner membrane and form a tripartite assembly with an outer membrane factor and a periplasmic linker in order to cross the two membranes to expulse molecules outside of the cell. A lot of information has been collected to understand the functional mechanism of these pumps, especially with AcrAB-TolC from Escherichia coli, but one missing piece from all the suggested models is the role of peptidoglycan in the assembly. Here, by pull-down experiments with purified peptidoglycans, we precise the MexAB-OprM interaction with the peptidoglycan from Escherichia coli and Pseudomonas aeruginosa, highlighting a role of the peptidoglycan in stabilizing the MexA-OprM complex and also differences between the two Gram-negative bacteria peptidoglycans.

scholarly journals Characterization of Integrons and Quinolone Resistance in Clinical Escherichia coli Isolates in Mansoura City, Egypt

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Shaymaa H. Abdel-Rhman ◽  
Rehab M. Elbargisy ◽  
Dina E. Rizk
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Efflux Pump ◽  
Quinolone Resistance ◽  
Aminoglycoside Resistance ◽  
Variable Regions ◽  
Active Efflux ◽  
E Coli ◽  
High Prevalence ◽  
Class I Integrons

Escherichia coli is a common pathogen in both humans and animals. Quinolones are used to treat infections caused by Gram-negative bacteria, but resistance genes emerged. Only scarce studies investigated the association between plasmid-mediated quinolone resistance (PMQR) genes and integrons in clinical isolates of E. coli. The current study investigated the prevalence of quinolone resistance and integrons among 134 clinical E. coli isolates. Eighty (59.70%) isolates were quinolone-resistant, and 60/134 (44.77%) isolates were integron positive with the predominance of class I integrons (98.33%). There was a significant association between quinolone resistance and the presence of integrons ( P < 0.0001 ). Isolates from Urology and Nephrology Center and Gastroenterology Hospital were significantly quinolone-resistant and integron positive ( P ≤ 0.0005 ). Detection of PMQR genes on plasmids of integron-positive isolates showed that the active efflux pump genes oqxAB and qepA had the highest prevalence (72.22%), followed by the aminoglycoside acetyltransferase gene (aac(6′)-Ib-cr, 66.67%) and the quinolone resistance genes (qnr, 61.11%). Amplification and sequencing of integrons’ variable regions illustrated that no quinolone resistance genes were detected, and the most predominant gene cassettes were for trimethoprim and aminoglycoside resistance including dfrA17, dfrB4, and dfrA17-aadA5. In conclusion, this study reported the high prevalence of PMQR genes and integrons among clinical E. coli isolates. Although PMQR genes are not cassette-born, they were associated with integrons’ presence, which contributes to the widespread of quinolone resistance in Egypt.

scholarly journals Involvement of Antibiotic Efflux Machinery in Glutathione-Mediated Decreased Ciprofloxacin Activity in Escherichia coli

2016 ◽  
Vol 60 (7) ◽  
pp. 4369-4374 ◽  
Author(s):  
Manish Goswami ◽  
Mahesh Subramanian ◽  
Ranjeet Kumar ◽  
Jana Jass ◽  
Narendra Jawali
Keyword(s):  
Escherichia Coli ◽  
Efflux Pump ◽  
Antibacterial Action ◽  
Potential Mechanism ◽  
Content Type ◽  
Active Efflux ◽  
E Coli ◽  
Acrab Efflux Pump ◽  
Antibiotic Efflux

ABSTRACTWe have analyzed the contribution of different efflux components to glutathione-mediated abrogation of ciprofloxacin's activity inEscherichia coliand the underlying potential mechanism(s) behind this phenomenon. The results indicated that glutathione increased the total active efflux, thereby partially contributing to glutathione-mediated neutralization of ciprofloxacin's antibacterial action inE. coli. However, the role of glutathione-mediated increased efflux becomes evident in the absence of a functional TolC-AcrAB efflux pump.

scholarly journals Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents

2017 ◽  
Vol 13 (4) ◽  
pp. 1538-1546 ◽  
Author(s):  
Tingting Zhang ◽  
Min Wang ◽  
Yixin Xie ◽  
Xianping Li ◽  
Zhihui Dong ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Antibacterial Agents ◽  
Efflux Pump ◽  
Active Efflux ◽  
Active Efflux Pump
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