scholarly journals Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.

2012 ◽  
Vol 11 (3) ◽  
pp. 3186-3197 ◽  
Author(s):  
S.M.B. Cação ◽  
T.F. Leite ◽  
I.G.F. Budzinski ◽  
T.B. dos Santos ◽  
M.B.S. Scholz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enzymatic Activity ◽  
Fruit Development ◽  
Coffea Arabica ◽  
Pectin Methylesterase ◽  
Fruit Development And Ripening ◽  
Coffea Arabica L

Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display

2002 ◽  
Vol 21 (1) ◽  
pp. 043-050 ◽  
Author(s):  
R Rojas-Herrera ◽  
F Quiroz-Figueroa ◽  
M Monforte-González ◽  
L Sánchez-Teyer ◽  
V M Loyola-Vargas
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Differential Gene Expression ◽  
Differential Display ◽  
Coffea Arabica ◽  
Rt Pcr ◽  
Differential Gene ◽  
Coffea Arabica L

Phylogenetic and expression analysis of protein disulfide isomerase unravels good reference genes for gene expression studies in pear and peach fruits

2018 ◽  
Vol 98 (5) ◽  
pp. 1045-1057
Author(s):  
Ya-Qi Ke ◽  
Hai-Yan Cheng ◽  
Xing Liu ◽  
Ming-Yue Zhang ◽  
Chao Gu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruit Development ◽  
Reference Genes ◽  
Protein Disulfide Isomerase ◽  
Fruit Trees ◽  
Disulfide Isomerase ◽  
Expression Studies ◽  
Fruit Development And Ripening ◽  
Gene Expression Studies ◽  
Protein Disulfide

Protein disulfide isomerase (PDI) is an important enzyme for protein folding in endoplasmic reticulum. The PDI gene family has been systematically studied in Arabidopsis, barley, rice, and bread wheat; however, little is known about this gene family and their roles during fruit development and ripening in fruit trees. In this study, 63 PDI genes were isolated from 8 fruit trees. Phylogenetic trees showed that these genes were clustered into six different groups, designated as A to F. In the groups, the PDI genes had significant differences in gene structure and conserved regions. The chromosome location of each PDI gene was determined in complete genome-assembly fruit trees and the synteny of chromosome segments was detected among peach, pear, and strawberry. Expression profiles of PDI genes in peach, pear, and strawberry showed that nearly all genes in group D and E were more highly expressed in developmental and ripening fruit tissues than those in other groups, while all genes in group A and B presented the lowest levels of expression in fruits of each stage. Moreover, qRT-PCR analyses revealed that these expressed genes were stable expressed in pear and peach fruits, as well as the reported reference genes. Eventually, PbPDI.F1 presented the highest expression stability in pear fruit while PpPDI.F displayed stronger stability than other genes in peach fruit. Thus, these two genes, which were clustered in group F, are good reference genes for gene expression studies during fruit development and ripening.

scholarly journals Sphingolipid Distribution, Content and Gene Expression during Olive-Fruit Development and Ripening

2018 ◽  
Vol 9 ◽  
Author(s):  
Carla Inês ◽  
Maria C. Parra-Lobato ◽  
Miguel A. Paredes ◽  
Juana Labrador ◽  
Mercedes Gallardo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruit Development ◽  
Olive Fruit ◽  
Fruit Development And Ripening

scholarly journals Developmental Variation in Fruit Polyphenol Content and Related Gene Expression of a Red-Fruited versus a White-Fruited Fragaria vesca Genotype

Horticulturae ◽  
2018 ◽  
Vol 4 (4) ◽  
pp. 30
Author(s):  
Sutapa Roy ◽  
Sanjay Singh ◽  
Douglas Archbold
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Fruit Development ◽  
Biosynthetic Pathway ◽  
Receptor Gene ◽  
Expression Patterns ◽  
Related Gene ◽  
Pathway Gene ◽  
Fruit Development And Ripening ◽  
Flavonoid Biosynthetic Pathway

Two cultivars of F. vesca, red-fruited Baron Solemacher (BS) and white-fruited Pineapple Crush (PC), were studied to compare and contrast the quantitative accumulation of major polyphenols and related biosynthetic pathway gene expression patterns during fruit development and ripening. Developing PC fruit showed higher levels of hydroxycinnamic acids in green stages and a greater accumulation of ellagitannins in ripe fruit in comparison to BS. In addition to anthocyanin, red BS fruit had greater levels of flavan-3-ols when ripe than PC. Expression patterns of key structural genes and transcription factors of the phenylpropanoid/flavonoid biosynthetic pathway, an abscisic acid (ABA) biosynthetic gene, and a putative ABA receptor gene that may regulate the pathway, were also analyzed during fruit development and ripening to determine which genes exhibited differences in expression and when such differences were first evident. Expression of all pathway genes differed between the red BS and white PC at one or more times during development, most notably at ripening when phenylalanine ammonia lyase 1 (PAL1), chalcone synthase (CHS), flavanone-3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), and UDP:flavonoid-O-glucosyltransferase 1 (UFGT1) were significantly upregulated in the red BS fruit. The transcription factors MYB1 and MYB10 did not differ substantially between red and white fruit except at ripening, when both the putative repressor MYB1 and promoter MYB10 were upregulated in red BS but not white PC fruit. The expression of ABA-related gene 9-cis-epoxycarotenoid dioxygenase 1 (NCED1) was higher in red BS fruit but only in the early green stages of development. Thus, a multigenic effect at several points in the phenylpropanoid/flavonoid biosynthetic pathway due to lack of MYB10 upregulation may have resulted in white PC fruit.

scholarly journals Photoperiod-dependent transcriptional modifications in key metabolic pathways in Coffea arabica

2020 ◽  
Author(s):  
Doâa Djerrab ◽  
Benoît Bertrand ◽  
Jean-Christophe Breitler ◽  
Sophie Léran ◽  
Eveline Dechamp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Metabolic Pathways ◽  
Coffea Arabica ◽  
Clock Genes ◽  
Tropical Species ◽  
Perennial Species ◽  
Natural Photoperiod ◽  
Coffea Arabica L ◽  
Short Days

Abstract Photoperiod length induces in temperate plants major changes in growth rates, morphology and metabolism with, for example, modifications in the partitioning of photosynthates to avoid starvation at the end of long nights. However, this has never been studied for a tropical perennial species adapted to grow in a natural photoperiod close to 12 h/12 h all year long. We grew Coffea arabica L., an understorey perennial evergreen tropical species in its natural 12 h/12 h and in a short 8 h/16 h photoperiod, and we investigated its responses at the physiological, metabolic and transcriptomic levels. The expression pattern of rhythmic genes, including core clock genes, was affected by changes in photoperiod. Overall, we identified 2859 rhythmic genes, of which 89% were also rhythmic in Arabidopsis thaliana L. Under short-days, plant growth was reduced, and leaves were thinner with lower chlorophyll content. In addition, secondary metabolism was also affected with chlorogenic acid and epicatechin levels decreasing, and in agreement, the genes involved in lignin synthesis were overexpressed and those involved in the flavanol pathway were underexpressed. Our results show that the 8 h/16 h photoperiod induces drastic changes in morphology, metabolites and gene expression, and the responses for gene expression are similar to those observed in the temperate annual A. thaliana species. Short photoperiod induces drastic changes in gene expression, metabolites and leaf structure, some of these responses being similar to those observed in A. thaliana.

scholarly journals Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening

2004 ◽  
Vol 136 (4) ◽  
pp. 4184-4197 ◽  
Author(s):  
Emma M. Eriksson ◽  
Arnaud Bovy ◽  
Ken Manning ◽  
Liz Harrison ◽  
John Andrews ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Fruit Development ◽  
Tomato Fruit ◽  
Fruit Development And Ripening
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