scholarly journals DNA barcoding of Oryx leucoryx using the mitochondrial cytochrome C oxidase gene

2012 ◽  
Vol 11 (1) ◽  
pp. 539-547 ◽  
Author(s):  
K. Elmeer ◽  
A. Almalki ◽  
K.A. Mohran ◽  
K.N. AL-Qahtani ◽  
M. Almarri
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Cytochrome ◽  
Oxidase Gene ◽  
Mitochondrial Cytochrome C

scholarly journals Novel Point Mutations and A8027G Polymorphism in Mitochondrial-DNA-Encoded Cytochrome c Oxidase II Gene in Mexican Patients with Probable Alzheimer Disease

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Verónica Loera-Castañeda ◽  
Lucila Sandoval-Ramírez ◽  
Fermín Paul Pacheco Moisés ◽  
Miguel Ángel Macías-Islas ◽  
Moisés Alejandro Alatorre Jiménez ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Alzheimer Disease ◽  
Cytochrome C Oxidase ◽  
Point Mutations ◽  
Mitochondrial Cytochrome ◽  
Catalytic Core ◽  
Oxidase Gene ◽  
Mexican Patients ◽  
Mitochondrial Cytochrome C

Mitochondrial dysfunction has been thought to contribute to Alzheimer disease (AD) pathogenesis through the accumulation of mitochondrial DNA mutations and net production of reactive oxygen species (ROS). Mitochondrial cytochrome c-oxidase plays a key role in the regulation of aerobic production of energy and is composed of 13 subunits. The 3 largest subunits (I, II, and III) forming the catalytic core are encoded by mitochondrial DNA. The aim of this work was to look for mutations in mitochondrial cytochrome c-oxidase gene II (MTCO II) in blood samples from probable AD Mexican patients.MTCO IIgene was sequenced in 33 patients with diagnosis of probable AD. Four patients (12%) harbored the A8027G polymorphism and three of them were early onset (EO) AD cases with familial history of the disease. In addition, other four patients with EOAD had only one of the following point mutations: A8003C, T8082C, C8201T, or G7603A. Neither of the point mutations found in this work has been described previously for AD patients, and the A8027G polymorphism has been described previously; however, it hasn’t been related to AD. We will need further investigation to demonstrate the role of the point mutations of mitochondrial DNA in the pathogenesis of AD.

Can DNA barcoding be used to identify closely related Clunio Haliday, 1855 species (Diptera: Chironomidae, Orthocladiinae)?

Zootaxa ◽  
2021 ◽  
Vol 4927 (1) ◽  
pp. 1-8
Author(s):  
PARASKEVA MICHALOVA ◽  
VALERIA LENCIONI ◽  
MOMCHIL NENOV ◽  
SVETOSLAV NIKOLOV
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Methodological Approach ◽  
Dna Barcode ◽  
Mitochondrial Cytochrome ◽  
Base Pairs ◽  
Oxidase Subunit ◽  
Gene Coi ◽  
Mitochondrial Cytochrome C

DNA barcoding based on a fragment of mitochondrial Cytochrome C Oxidase subunit 1 gene (COI) was applied to the two chironomids Clunio balticus Heimbach (690 base pairs) and C. ponticus Michailova (691 base pairs). The two species differed by one deletion in the nucleotide sequence Adenine. However, the 658-nucleotide long sequences of DNA from the mitochondrial Cytochrome C Oxidase subunit 1 gene (COI) of C. balticus and C. ponticus were identical upon comparison. Further, they compared with homologous sequences for C. marinus Holiday and C. tsushimensis Tokunaga from the Barcode of Life (BOLD) database and the results plotted as a weighted graph, where C. tsushimensis, C. marinus and C. balticus C. ponticus formed three almost equidistant groups. From this, we established that the genetic distance between the respective COI sequences of C. balticus and C. ponticus is minimal, indicating a close relationship between the species indicative of recent common origin. However, the comparative analysis between C. tsushimensis, C. marinus, C. balticus and C. ponticus showed a wider divergence in their respective nucleotide sequences. Overall, our results emphasized that the COI region does not work well as a DNA barcode to identify species within the Clunio genus. Either longer sequences or a multifaceted methodological approach, including morphology, cytogenetic and ecology is needed to distinguish some members of Clunio genus.  

scholarly journals Taxonomy and the DNA Barcoding Enterprise

Zootaxa ◽  
2011 ◽  
Vol 2742 (1) ◽  
pp. 67 ◽  
Author(s):  
MALTE C. EBACH
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Type Specimen ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Oxidase Subunit ◽  
Mitochondrial Cytochrome C

DNA Barcoding is elusive to many taxonomists. Like the numbers in a barcode, barcoding attempts to link a type specimen with a part of its DNA, most commonly from the mitochondrial Cytochrome c Oxidase subunit I (COI) gene.

Redescription of Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Acari: Trombidiformes: Stigmaeidae) with DNA barcoding

2019 ◽  
Vol 24 (1) ◽  
pp. 33
Author(s):  
Mohamed W. Negm ◽  
Tetsuo Gotoh
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Predatory Mites ◽  
Mitochondrial Cytochrome ◽  
Morphological Variations ◽  
Oxidase Subunit ◽  
Mitochondrial Cytochrome C

Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Stigmaeidae) are cosmopolitan predatory mites and appear on various plants in Japan. They are so close morphologically and can be mainly separated based on the lengths of dorsal setae sci and c2. The present study aimed to redescribe these two species for detailed morphological variations and generate sequences of partial mitochondrial cytochrome c oxidase subunit I (COI) as DNA barcode data. Our results with DNA barcoding and morphology well separated A. lobatus from the close species A. terminalis.

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