scholarly journals Cloning of a phosphatidylinositol 4-kinase gene based on fiber strength transcriptome QTL mapping in the cotton species Gossypium barbadense

2012 ◽  
Vol 11 (3) ◽  
pp. 3367-3378 ◽  
Author(s):  
H.W. Liu ◽  
R.F. Shi ◽  
X.F. Wang ◽  
Y.X. Pan ◽  
G.Y. Zang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Fiber Strength ◽  
Gossypium Barbadense ◽  
Kinase Gene ◽  
Cotton Species ◽  
Phosphatidylinositol 4

scholarly journals Genome-Wide Mining and Identification of Protein Kinase Gene Family Impacts Salinity Stress Tolerance in Highly Dense Genetic Map Developed from Interspecific Cross between G. hirsutum L. and G. darwinii G. Watt

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 560 ◽  
Author(s):  
Muhammad Shehzad ◽  
Zhongli Zhou ◽  
Allah Ditta ◽  
Xiaoyan Cai ◽  
Majid Khan ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Genetic Map ◽  
Interspecific Cross ◽  
Genetic Erosion ◽  
Growth And Yield ◽  
Limiting Factor ◽  
Kinase Gene ◽  
Cotton Species

Abiotic stress is an important limiting factor in crop growth and yield around the world. Owing to the continued genetic erosion of the upland cotton germplasm due to intense selection and inbreeding, attention has shifted towards wild cotton progenitors which offer unique traits that can be introgressed into the cultivated cotton to improve their genetic performance. The purpose of this study was to characterize the Pkinase gene family in a previously developed genetic map of the F2 population derived from a cross between two cotton species: Gossypium hirsutum (CCRI 12-4) and Gossypium darwinii (5-7). Based on phylogenetic analysis, Pkinase (PF00069) was found to be the dominant domain with 151 genes in three cotton species, categorized into 13 subfamilies. Structure analysis of G. hirsutum genes showed that a greater percentage of genes and their exons were highly conserved within the group. Syntenic analysis of gene blocks revealed 99 duplicated genes among G. hirsutum, Gossypium arboreum and Gossypium raimondii. Most of the genes were duplicated in segmental pattern. Expression pattern analysis showed that the Pkinase gene family possessed species-level variation in induction to salinity and G. darwinii had higher expression levels as compared to G. hirsutum. Based on RNA sequence analysis and preliminary RT-qPCR verification, we hypothesized that the Pkinase gene family, regulated by transcription factors (TFs) and miRNAs, might play key roles in salt stress tolerance. These findings inferred comprehensive information on possible structure and function of Pkinase gene family in cotton under salt stress.

Association between Alternaria macrospora and Alternaria alternata, causal agents of cotton leaf blight

1991 ◽  
Vol 69 (12) ◽  
pp. 2603-2607 ◽  
Author(s):  
Yoav Bashan ◽  
Hanna Levanony ◽  
Reuven Or
Keyword(s):  
Gossypium Hirsutum ◽  
Alternaria Alternata ◽  
Leaf Surface ◽  
Gossypium Barbadense ◽  
Cotton Leaf ◽  
Optimal Dosage ◽  
Alternaria Blight ◽  
Cotton Species ◽  
Infested Field ◽  
Blight Disease

The association between Alternaria macrospora and Alternaria alternata, responsible for the development of alternaria blight disease in cotton, was evaluated in artificially inoculated greenhouse plants and in naturally infested field plants. When greenhouse plants were inoculated with suboptimal doses of both pathogens (< 1.2 × 104 spores/mL) infection was greater than when separately inoculated by each pathogen at optimal dosage. In field-grown, naturally infected plants (Gossypium barbadense), both pathogens were found together in more than 40% of the plants. A second field-grown cotton species (Gossypium hirsutum) exhibited infection mainly by either A. alternata or both pathogens together. When both cotton species were naturally infected by both pathogens together, the number of A. alternata spores (either airborne or on the leaf surface) was greater than that of A. macrospora. We propose that A. macrospora together with A. alternata create a disease composite responsible for alternaria blight symptoms in cotton. Key words: Alternaria, cotton diseases, Gossypium barbadense, Gossypium hirsutum.

Specific attraction to and infection of cotton root cap cells by zoospores of Pythium dissotocum

1989 ◽  
Vol 67 (6) ◽  
pp. 1760-1767 ◽  
Author(s):  
N. P. Goldberg ◽  
M. C. HAwes ◽  
M. E. Stanghellini
Keyword(s):  
Living Cells ◽  
Gossypium Barbadense ◽  
Root Cap ◽  
Chemotactic Response ◽  
Agrostis Palustris ◽  
Isolated Cells ◽  
Cotton Species ◽  
Pythium Dissotocum ◽  
Zoospore Suspension ◽  
Individual Root

Root cap cells of two cotton species (Gossypium barbadense L. and G. hirsutum L.) elicited a specific chemotactic response in zoospores of Pythium dissotocum. When roots of cotton seedlings were placed into a suspension of Pythium dissotocum zoospores, there was immediate attraction, accumulation, and encystment exclusively in the root cap region. Seedlings which attracted zoospores were killed within 24 h. Furthermore, root cap cells remained attractive when isolated nondestructively from the root and placed into a zoospore suspension; attraction, accumulation, and encystment on individual root cap cells occurred within seconds after contact. Penetration and death of isolated cells occurred within 15–30 min. After 30 min, approximately 25% of living cells were directly colonized by zoospores. Root cap cells killed by freezing or drying remained attractive but at a reduced level; approximately half as many killed cells as living cells were directly colonized by zoospores. The number of root cap cells directly colonized by zoospores did not increase with time. In contrast, zoospores of Pythium catenulatum that exhibited a chemotactic response to Agrostis palustris (Bentgrass) were not attracted to and did not infect cotton seedlings or isolated root cap cells.

scholarly journals Genetic Diversity, QTL Mapping, and Marker-Assisted Selection Technology in Cotton (Gossypium spp.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Fakhriddin N. Kushanov ◽  
Ozod S. Turaev ◽  
Dilrabo K. Ernazarova ◽  
Bunyod M. Gapparov ◽  
Barno B. Oripova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Qtl Mapping ◽  
Dna Markers ◽  
Marker Assisted Selection ◽  
Large Scale ◽  
Genome Wide Association Study ◽  
Fiber Quality ◽  
Cotton Species ◽  
Analysis Application ◽  
Or Gene

Cotton genetic resources contain diverse economically important traits that can be used widely in breeding approaches to create of high-yielding elite cultivars with superior fiber quality and adapted to biotic and abiotic stresses. Nevertheless, the creation of new cultivars using conventional breeding methods is limited by the cost and proved to be time consuming process, also requires a space to make field observations and measurements. Decoding genomes of cotton species greatly facilitated generating large-scale high-throughput DNA markers and identification of QTLs that allows confirmation of candidate genes, and use them in marker-assisted selection (MAS)-based breeding programs. With the advances of quantitative trait loci (QTL) mapping and genome-wide-association study approaches, DNA markers associated with valuable traits significantly accelerate breeding processes by replacing the selection with a phenotype to the selection at the DNA or gene level. In this review, we discuss the evolution and genetic diversity of cotton Gossypium genus, molecular markers and their types, genetic mapping and QTL analysis, application, and perspectives of MAS-based approaches in cotton breeding.

scholarly journals Evolution of pectin synthesis relevant galacturonosyltransferase gene family and its expression during cotton fiber development

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Senmiao FAN ◽  
Aiying LIU ◽  
Xianyan ZOU ◽  
Zhen ZHANG ◽  
Qun GE ◽  
...  
Keyword(s):  
Gene Family ◽  
Cotton Fiber ◽  
Fiber Strength ◽  
Expression Patterns ◽  
Fiber Development ◽  
Fiber Elongation ◽  
Transcript Levels ◽  
Cotton Fiber Development ◽  
Conserved Sequence ◽  
Cotton Species

Abstract Background Pectin is a key substance involved in cell wall development, and the galacturonosyltransferases (GAUTs) gene family is a critical participant in the pectin synthesis pathway. Systematic and comprehensive research on GAUTs has not been performed in cotton. Analysis of the evolution and expression patterns of the GAUT gene family in different cotton species is needed to increase knowledge of the function of pectin in cotton fiber development. Results In this study, we have identified 131 GAUT genes in the genomes of four Gossypium species (G. raimondii, G. barbadense, G. hirsutum, and G. arboreum), and classified them as GAUT-A, GAUT-B and GAUT-C, which coding probable galacturonosyltransferases. Among them, the GAUT genes encode proteins GAUT1 to GAUT15. All GAUT proteins except for GAUT7 contain a conserved glycosyl transferase family 8 domain (H-DN-A-SVV-S-V-H-T-F). The conserved sequence of GAUT7 is PLN (phospholamban) 02769 domain. According to cis-elemet analysis, GAUT genes transcript levels may be regulated by hormones such as JA, GA, SA, ABA, Me-JA, and IAA. The evolution and transcription patterns of the GAUT gene family in different cotton species and the transcript levels in upland cotton lines with different fiber strength were analyzed. Peak transcript level of GhGAUT genes have been observed before 15 DPA. In the six materials with high fiber strength, the transcription of GhGAUT genes were concentrated from 10 to 15 DPA; while the highest transcript levels in low fiber strength materials were detected between 5 and 10 DPA. These results lays the foundation for future research on gene function during cotton fiber development. Conclusions The GAUT gene family may affect cotton fiber development, including fiber elongation and fiber thickening. In the low strength fiber lines, GAUTs mainly participate in fiber elongation, whereas their major effect on cotton with high strength fiber is related to both elongation and thickening.

Sign in / Sign up

Export Citation Format

Share Document