scholarly journals Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis

2012 ◽  
Vol 11 (1) ◽  
pp. 30-41 ◽  
Author(s):  
M.A. Latif ◽  
M.Y. Omar ◽  
S.G. Tan ◽  
S.S. Siraj ◽  
M.E. Ali ◽  
...  
Keyword(s):  
Host Plants ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Pcr Analysis ◽  
Sympatric Populations ◽  
Rapd Pcr

Feeding responses of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), to resistant and susceptible host-plants

1989 ◽  
Vol 79 (2) ◽  
pp. 309-318 ◽  
Author(s):  
D. E. Padgham ◽  
S. Woodhead ◽  
H. R. Rapusas
Keyword(s):  
Resistance Genes ◽  
Host Plants ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Resistance Mechanisms ◽  
Population Development ◽  
Rice Varieties ◽  
Susceptible Host ◽  
Insect Activity ◽  
Nilaparvata Lugens Stål

AbstractThe feeding responses, growth and population development of Nilaparvata lugens (Stål) are quantified on a range of twelve susceptible or resistant host-plants. Paris of rice varieties carrying the Bph1, bph2 and Bph3 resistance genes are compared as hosts for N. lugens, and it is concluded that such notations do not adequately describe the diversity of plant resistance mechanisms. Evidence is presented for resistance mechanisms involving enhanced insect activity and gustatory responses to unacceptable phloem.

scholarly journals Effects of Different Nutritional Conditions on the Growth and Reproduction of Nilaparvata lugens (Stål)

2022 ◽  
Vol 12 ◽  
Author(s):  
Kui Kang ◽  
Youjun Cai ◽  
Lei Yue ◽  
Wenqing Zhang
Keyword(s):  
Nutrient Concentration ◽  
Fat Body ◽  
Brown Planthopper ◽  
Total Lipid Content ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Nutritional Deficiencies ◽  
Pcr Analysis ◽  
Nutritional Conditions ◽  
Growth And Reproduction

Growth and reproduction are the two most basic life processes of organisms and the distribution of energy in these processes is a core issue of the life history of organisms. Nilaparvata lugens (Stål), the brown planthopper (BPH), is a single-feeding rice pest. In the present study, this species was used as a model for testing the effects of nutritional conditions on various growth and reproduction indicators. First, the third-instar nymphs were fed with three different concentrations (100, 50, and 25%) of artificial diet until the second day of adulthood. The results showed that as the nutrient concentration decreased, the body development and oviposition of BPH were hindered. The total lipid content in the fat bodies was also significantly reduced. RT-PCR analysis showed compared to the 100% concentration group, the expression levels of vitellogenin (Vg) genes in the fifth-instar nymphs, adults, and in different tissues (ovary, fat body, and other tissues) were significantly decreased in the 50 and 25% treatment groups. Western blot analysis showed that Vg protein expression was highest in the 100% group, followed by the 50% group, with no expression in the 25% group. These results indicate that growth and reproduction in the BPH are regulated by, or correlated with, nutrient concentration. This study is of great significance as it reveals the adaptive strategies of the BPH to nutritional deficiencies and it also provides valuable information for the comprehensive control of this pest.

scholarly journals Genetic Dissection of Sympatric Populations of Brown Planthopper,Nilaparvata lugens(Stål), Using DALP-PCR Molecular Markers

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
M. A. Latif ◽  
M. Y. Rafii ◽  
M. S. Mazid ◽  
M. E. Ali ◽  
F. Ahmed ◽  
...  
Keyword(s):  
Genetic Polymorphisms ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Genetic Distances ◽  
The Other ◽  
Oligonucleotide Primer ◽  
Group Method ◽  
Genetic Dissection ◽  
Sympatric Populations ◽  
Esterase Activities

Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper,Nilaparvata lugensspecies complex for the analysis of genetic polymorphisms. A total of 11 populations ofNilaparvataspp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper,N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations.N. bakeriwas used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations ofNilaparvataspp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations ofN. lugensformed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured fromL. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis.

Genetic analysis of brown planthopper biotypes using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)

1995 ◽  
Vol 16 (1) ◽  
pp. 27-33 ◽  
Author(s):  
K. A. Shufran ◽  
M. E. Whalon
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Random Amplified Polymorphic Dna ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
The Philippines ◽  
Rice Cultivars ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Polymerase Chain

AbstractRandom amplified polymorphic DNA (RAPD) amplified by the polymerase chain reaction (PCR) was used to estimate the relatedness of three biotypes of brown planthopper, Nilaparvata lugens (Stål), from the Philippines. Each individual brown planthopper had a unique set of RAPD bands, but no bands were diagnostic for any one biotype. Brown planthopper types 1, 2 and 3 were genetically homogeneous. This supports results of other studies which conclude that brown planthopper biotypes are not sub-specific categories, but merely represent individual variation for virulence to rice cultivars.

Screening of rice genotypes for resistance to the brown planthopper, Nilaparvata lugens Stål

2012 ◽  
Vol 40 (4) ◽  
pp. 502-508 ◽  
Author(s):  
M. P. Ali ◽  
Salem S. Alghamdi ◽  
M. A. Begum ◽  
A. B. M. Anwar Uddin ◽  
M. Z. Alam ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Genotypes

Molecular characterization of insulin‐like peptides in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

2020 ◽  
Vol 29 (3) ◽  
pp. 309-319 ◽  
Author(s):  
W.‐H. Xue ◽  
Y.‐L. Liu ◽  
Y.‐Q. Jiang ◽  
S.‐F. He ◽  
Q.‐Q. Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Brown Planthopper ◽  
Nilaparvata Lugens
Sign in / Sign up

Export Citation Format

Share Document