scholarly journals Isolation and characterization of nucleotide-binding site and C-terminal leucine-rich repeat-resistance gene candidates in bananas

2011 ◽  
Vol 10 (4) ◽  
pp. 3098-3108 ◽  
Author(s):  
Y. Lu ◽  
W.H. Xu ◽  
Y.X. Xie ◽  
X. Zhang ◽  
J.J. Pu ◽  
...  
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
Isolation And Characterization ◽  
Resistance Gene Candidates

A superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 782-788 ◽  
Author(s):  
W Spielmeyer ◽  
M Robertson ◽  
N Collins ◽  
D Leister ◽  
P Schulze-Lefert ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
Leucine Rich Repeat ◽  
Homoeologous Chromosome ◽  
Entry Points

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

scholarly journals Mutational Analysis of the Arabidopsis Nucleotide Binding Site–Leucine-Rich Repeat Resistance Gene RPS2

2000 ◽  
Vol 12 (12) ◽  
pp. 2541-2554 ◽  
Author(s):  
Yi Tao ◽  
Fenghua Yuan ◽  
R. Todd Leister ◽  
Frederick M. Ausubel ◽  
Fumiaki Katagiri
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
Mutational Analysis ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat

Characterization of resistance gene analogs with a nucleotide binding site isolated from a triploid white poplar

Plant Biology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 310-322 ◽  
Author(s):  
Q. Zhang ◽  
Z.-Y. Zhang ◽  
S.-Z. Lin ◽  
H.-Q. Zheng ◽  
Y.-Z. Lin ◽  
...  
Keyword(s):  
Binding Site ◽  
Resistance Gene ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
White Poplar

scholarly journals Isolation and Characterization of Nucleotide-Binding Site Resistance Gene Homologues in Common Bean (Phaseolus vulgaris)

2013 ◽  
Vol 103 (2) ◽  
pp. 156-168 ◽  
Author(s):  
Luz N. Garzón ◽  
Oscar A. Oliveros ◽  
Benjamin Rosen ◽  
Gustavo A. Ligarreto ◽  
Douglas R. Cook ◽  
...  
Keyword(s):  
Common Bean ◽  
Binding Site ◽  
Resistance Gene ◽  
Focal Point ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
R Genes ◽  
Degenerate Primers ◽  
Model Legume ◽  
Isolation And Characterization

Common bean production is constrained by many fungal, viral, and bacterial pathogens. Thus, the identification of resistance (R) genes is an important focal point of common bean research. The main goal of our study was to identify resistance gene homologues (RGH) in the crop, using degenerate primers designed from conserved sequences in the nucleotide-binding site (NBS) domains of R-genes from the model legume Medicago truncatula. Total DNA of the Andean common bean genotype G19833 was used for amplification of over 500 primer combinations. Sequencing of amplicons showed that 403 cloned fragments had uninterrupted open reading frames and were considered representative of functional RGH genes. The sequences were grouped at two levels of nucleotide identity (90 and 80%) and representative sequences of each group were used for phylogenetic analyses. The RGH sequence diversity of common bean was divided into TIR and non-TIR families, each with different clusters. The TIR sequences grouped into 14 clades while non-TIR sequences grouped into seven clades. Pairwise comparisons showed purifying selection, although some sequences may have been the result of diversifying selection. Knowledge about RGH genes in common bean can allow the design of molecular markers for pyramiding of resistance genes against various pathogens.

scholarly journals Identification and Characterization of Nucleotide-Binding Site-Leucine-Rich Repeat Genes in the Model Plant Medicago truncatula

2007 ◽  
Vol 146 (1) ◽  
pp. 5-21 ◽  
Author(s):  
Carine Ameline-Torregrosa ◽  
Bing-Bing Wang ◽  
Majesta S. O'Bleness ◽  
Shweta Deshpande ◽  
Hongyan Zhu ◽  
...  
Keyword(s):  
Binding Site ◽  
Medicago Truncatula ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
Model Plant ◽  
Identification And Characterization
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