scholarly journals Use of Cryopreserved Osteogenic Matrix Cell Sheets for Bone Reconstruction

2016 ◽  
Vol 06 (01) ◽  
pp. 13-23 ◽  
Author(s):  
Tomohiko Kura ◽  
Manabu Akahane ◽  
Takamasa Shimizu ◽  
Yoshinobu Uchihara ◽  
Yasuaki Tohma ◽  
...  
Keyword(s):  
Bone Reconstruction ◽  
Cell Sheets ◽  
Matrix Cell

Liver bud transplantation in rats

2018 ◽  
Vol 71 (4) ◽  
pp. 163-169
Author(s):  
Eiji Kobayashi ◽  
Shin Enosawa
Keyword(s):  
Fetal Liver ◽  
Liver Parenchyma ◽  
Arterial Blood Flow ◽  
Silk Suture ◽  
Cell Sheets ◽  
Human Liver Tissue ◽  
Arterial Blood ◽  
Matrix Cell ◽  
Experimental Microsurgery

Abstract: Introduction: Research has made progress in organ fabrication using an extracellular matrix, cell sheets, or organoids. Human liver tissue has been constructed using a 3-dimensional (3D) bioprinter and showed evidence that an in vitro generated liver bud was reformed in a rodent liver model. This study describes the stages of development of rat fetal organs and liver structure and reviews recent progress in liver organoid transplantation. Methods: The authors developed the procedures for creating a transected plane for use in experimental microsurgery in rats. A liver lobe was fixed vertically with gauze and it was ligated with 6-0 silk suture in the cut line; the parenchyma was cut, and major vessels were ligated to create the transected plane. The ligated tissue was carefully resected. Hemostasis was not required and hepatic components remained on the transected plane. The plane was covered by omentum. Results: Using this model, we transplanted fetal liver or a 3D bioprinted liver organoid. This microsurgical method enabled creation of an intact liver parenchyma plane. No bleeding was observed. The transplanted liver components successfully engrafted on the liver. Conclusion: This method may provide an essential environment for growing liver using portal and arterial blood flow.

Bone Reconstruction Utilizing Mesenchymal Stem Cell Sheets for Cell Delivery

2012 ◽  
pp. 131-135
Author(s):  
Zou Xiao Hui ◽  
Shen Wei Liang ◽  
Boon Chin Heng ◽  
Ouyang Hong Wei
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Delivery ◽  
Bone Reconstruction ◽  
Cell Sheets

scholarly journals Maxillofacial bone regeneration with osteogenic matrix cell sheets: An experimental study in rats

2016 ◽  
Vol 72 ◽  
pp. 138-145 ◽  
Author(s):  
Yoshihiro Ueyama ◽  
Takahiro Yagyuu ◽  
Masahiko Maeda ◽  
Mitsuhiko Imada ◽  
Manabu Akahane ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Regeneration ◽  
Cell Sheets ◽  
Matrix Cell

scholarly journals Promotion of Osteogenesis and Angiogenesis in Vascularized Tissue-Engineered Bone Using Osteogenic Matrix Cell Sheets

2016 ◽  
Vol 137 (5) ◽  
pp. 1476-1484 ◽  
Author(s):  
Kenichi Nakano ◽  
Keiichi Murata ◽  
Shohei Omokawa ◽  
Manabu Akahane ◽  
Takamasa Shimizu ◽  
...  
Keyword(s):  
Cell Sheets ◽  
Matrix Cell

scholarly journals Osteogenic Matrix Cell Sheets Facilitate Osteogenesis in Irradiated Rat Bone

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Yoshinobu Uchihara ◽  
Manabu Akahane ◽  
Takamasa Shimizu ◽  
Tomoyuki Ueha ◽  
Yusuke Morita ◽  
...  
Keyword(s):  
Treatment Option ◽  
Callus Formation ◽  
Cell Sheet ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Cell Sheets ◽  
Musculoskeletal Tumors ◽  
Computed Tomography Images ◽  
Matrix Cell

Reconstruction of large bone defects after resection of malignant musculoskeletal tumors is a significant challenge in orthopedic surgery. Extracorporeal autogenous irradiated bone grafting is a treatment option for bone reconstruction. However, nonunion often occurs because the osteogenic capacity is lost by irradiation. In the present study, we established an autogenous irradiated bone graft model in the rat femur to assess whether osteogenic matrix cell sheets improve osteogenesis of the irradiated bone. Osteogenic matrix cell sheets were prepared from bone marrow-derived stromal cells and co-transplanted with irradiated bone. X-ray images at 4 weeks after transplantation showed bridging callus formation around the irradiated bone. Micro-computed tomography images at 12 weeks postoperatively showed abundant callus formation in the whole circumference of the irradiated bone. Histology showed bone union between the irradiated bone and host femur. Mechanical testing showed that the failure force at the irradiated bone site was significantly higher than in the control group. Our study indicates that osteogenic matrix cell sheet transplantation might be a powerful method to facilitate osteogenesis in irradiated bones, which may become a treatment option for reconstruction of bone defects after resection of malignant musculoskeletal tumors.

scholarly journals Osteogenesis of osteogenic matrix cell sheets preserved in culture medium in a rat model

2018 ◽  
Vol 27 (8) ◽  
pp. 1281-1288
Author(s):  
Tsutomu Kira ◽  
Manabu Akahane ◽  
Noriko Ouji-Sageshima ◽  
Takamasa Shimizu ◽  
Tadanobu Onishi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Culture Medium ◽  
Stem Cell Markers ◽  
Cell Sheets ◽  
Cytotoxic Effects ◽  
Matrix Cell ◽  
Significant Difference ◽  
Preservation Conditions ◽  
Ldh Release ◽  
Stromal Stem Cells

Osteogenic matrix cell sheets (OMCSs) are ideal for bone regeneration. Transportation of OMCSs may be necessary, during which their osteogenic ability must be maintained. Here, we evaluated different media and temperatures for OMCS preservation. Bone marrow stromal/stem cells (BMSCs) were obtained from Fischer rats and analyzed for stem cell markers by flow cytometry. OMCSs were prepared from BMSCs by treatment with dexamethasone and ascorbic acid phosphate. After OMCS collection, they were stored in minimum essential medium (MEM) or Hank’s balanced salt solution (HBSS) at 37, 22, or 4°C for 24 hours. Cell viability and cytotoxic effects in the preservation conditions were determined by adenosine triphosphate (ATP) contents and lactate dehydrogenase (LDH) release, respectively. Osteogenesis was assessed by subcutaneously implanting preserved OMCSs around β-tricalcium phosphate ceramic disks into syngeneic rats. Implants were evaluated by alkaline phosphatase (ALP) activities, osteocalcin contents, and histology. Mesenchymal stem cells comprised 51% of primary cultured BMSCs. ATP contents were significantly different in OMCSs stored in MEM or HBSS at 22°C and 4°C. LDH release was significantly different in OMCSs stored in HBSS at 22°C and 4°C. The highest LDH release was observed in OMCSs stored in HBSS at 37°C. ALP activities and osteocalcin contents were the lowest in implanted OMCSs stored in HBSS at 37°C at four weeks after subcutaneous implantation. There was a significant difference in the osteocalcin levels of implanted OMCSs stored in MEM at 37°C and HBSS at 4°C. Abundant bone tissue around and inside disks was found in histological sections of OMCSs stored in all preservation conditions except for MEM and HBSS at 37°C. Maintaining the osteogenic ability of OMCSs during transport is important, and preservation of OMCSs in MEM or HBSS at 4°C or 22°C is a simple and inexpensive method.

0steogenic potential of the osteogenic matrix cell sheets in maxillofacial regeneration

2014 ◽  
Vol 72 (9) ◽  
pp. e199-e200
Author(s):  
Y. Ueyama ◽  
T. Yagyuu ◽  
M. Maeda ◽  
T. Kirita
Keyword(s):  
Cell Sheets ◽  
Matrix Cell

scholarly journals Osteogenesis of cryopreserved osteogenic matrix cell sheets

Cryobiology ◽  
2013 ◽  
Vol 66 (3) ◽  
pp. 326-332 ◽  
Author(s):  
Takamasa Shimizu ◽  
Manabu Akahane ◽  
Tomoyuki Ueha ◽  
Akira Kido ◽  
Shohei Omokawa ◽  
...  
Keyword(s):  
Cell Sheets ◽  
Matrix Cell

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

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