scholarly journals Localization of the VP2 Protein of Canine Parvovirus Type 2 on the Baculovirus Envelop and Its Immunogenicity in a Mouse Model

2012 ◽  
Vol 02 (04) ◽  
pp. 178-185 ◽  
Author(s):  
Chih H. Tsai ◽  
Jing Y. Wang ◽  
Xin G. Xu ◽  
De W. Tong ◽  
Hsin Y. Lu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Canine Parvovirus ◽  
Vp2 Protein ◽  
Canine Parvovirus Type 2

scholarly journals Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland

2019 ◽  
Vol 69 (1) ◽  
pp. 32-46 ◽  
Author(s):  
Marek Kowalczyk ◽  
Barbara Majer-Dziedzic ◽  
Krzysztof Kostro ◽  
Aleksandra Szabelak ◽  
Jerzy Ziętek ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Position ◽  
Canine Parvovirus ◽  
Vp2 Protein ◽  
Pcr Product ◽  
Common Causes ◽  
Preventive Vaccination ◽  
High Degree ◽  
Canine Parvovirus Type 2

Abstract Canine parvovirus type 2 is one of the most common causes of death among puppies. Despite preventive vaccination, the disease continues to be diagnosed. The aim of the study was to provide a molecular characterization of CPV-2 isolates found in southeastern Poland. Genetic CPV-2 material was isolated from the blood (n=10) and feces (n=50) of infected dogs. The presence of CPV-2 was confirmed by amplification of sequences coding both VP1 and VP2 protein. The products of the PCR reaction with primers amplifying VP2 protein were sequenced and used for genotyping. Bioinformatics analysis of the sequenced PCR product was performed to determine the phylogenetic relationships with variants recorded in the public databases. Based on the analysis of polymorphism in the nucleotide sequence 7 nucleotide variants were detected and assigned into four amino acid groups. Representatives of three groups contained asparagine at amino acid position 426 of the VP2 protein, which is characteristic of CPV-2a. The variant from the fourth group belonged to type CPV-2b. CPV-2a is the dominant antigenic type of CPV-2 in Poland. The pathogen’s high degree of polymorphism is manifested not only by the presence of numerous variants within the type, but also by the presence of representatives of type CPV-2b. Further studies of the molecular epidemiology of CPV-2 are necessary to optimize the effectiveness of preventive measures.

scholarly journals Risk and Environmental Factors Associated with the Presence of Canine Parvovirus Type 2 in Diarrheic Dogs from Thessaly, Central Greece

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 590
Author(s):  
Maria Kantere ◽  
Labrini V. Athanasiou ◽  
Alexios Giannakopoulos ◽  
Vassilis Skampardonis ◽  
Marina Sofia ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Clinical Symptoms ◽  
Gastrointestinal Disease ◽  
Environmental Parameters ◽  
Canine Parvovirus ◽  
Geographical Information ◽  
Factors Associated ◽  
Working Dogs ◽  
Canine Parvovirus Type 2

Canine parvovirus type 2 (CPV-2) primarily infects dogs, which are the main host reservoir, causing severe gastrointestinal disease associated with immunosuppression. The present study was conducted in Thessaly, Greece and aimed to identify risk and environmental factors associated with CPV-2 infection in diarrheic dogs. Fecal samples were collected from 116 dogs presenting diarrhea and were tested by polymerase chain reaction (PCR) for the presence of CPV-2 DNA. Supplementary data regarding clinical symptoms, individual features, management factors and medical history were also gathered for each animal during clinical evaluation. Sixty-eight diarrheic dogs were found to be positive for the virus DNA in their feces. Statistical analysis revealed that CPV-2 DNA was less likely to be detected in senior dogs, while working dogs, namely hounds and shepherds, had higher odds to be positive for the virus. Livestock density and land uses, specifically the categories of discontinuous urban fabric and of human population density, were identified as significant environmental parameters associated with CPV-2 infection by using Geographical Information System (GIS) together with the Ecological Niche Model (ENM). This is the first description of the environmental variables associated with the presence of CPV-2 DNA in dogs’ feces in Greece.

Isolation of a virus related to canine parvovirus type 2 from a raccoon (Procyon lotor)

2010 ◽  
Vol 166 (1) ◽  
pp. 24-25 ◽  
Author(s):  
S. Kapil ◽  
G. Rezabek ◽  
B. Germany ◽  
L. Johnston
Keyword(s):  
Procyon Lotor ◽  
Canine Parvovirus ◽  
Canine Parvovirus Type 2

scholarly journals Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019

2020 ◽  
Vol 64 (4) ◽  
pp. 477-486
Author(s):  
Wen Hu ◽  
Liangyan Zheng ◽  
Xin Xu ◽  
Qiang Liu ◽  
Jun Ji ◽  
...  
Keyword(s):  
Eastern China ◽  
Canine Parvovirus ◽  
Zhejiang Province ◽  
Central China ◽  
High Morbidity ◽  
Faecal Samples ◽  
Amino Acid Mutations ◽  
Canine Parvovirus Type 2 ◽  
Major Amino Acid

AbstractIntroductionCanine parvovirus type-2 (CPV-2) causes acute infectious diseases in puppies, which show high morbidity and mortality. Better effect of vaccination against these diseases could be achieved with deeper knowledge of CPV-2 genotype dissemination and mutation history. This study investigated CPV-2–positive samples collected recently over a wide region of China.Material and MethodsA total of 118 faecal samples from dogs identified as CPV-positive were collected from veterinary clinics in central and eastern China. Overall, 16 strains collected from Anhui, 29 from Henan, and 16 from Zhejiang Province were sequenced to determine the genotypic composition of CPV-2 and mutational complexity of CPV-VP2.ResultsThe CPV-2a, CPV-2b, and CPV-2c genotypes were detected in Anhui and Henan Provinces, while CPV-2c alone was detected in Zhejiang Province. Sequence analysis of all strains showed 98.5%–99.8%, 98.3%–99.9%, and 98.7%–99.8% identity among the 16 Anhui, 29 Henan, and 16 Zhejiang strains, respectively. Strains collected from Anhui and Henan Provinces showed lower identity (97.0%), suggesting greater genetic divergence in central China. The mutation rates of Henan and Anhui strains were lower than that of Zhejiang strains. Major amino acid mutations occurred at sites 5, 370, 426, and 440. Epitope and entropy analyses implied these sites’ likely conformance to the principles of mutation tendency, complexity, and diversity.ConclusionThe findings for the evolutionary structure of CPV-2 strains collected from three provinces in central and eastern China advance trend monitoring of the genetic variation in canine parvovirus and point to its implications in the development of novel vaccines.

scholarly journals A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

2013 ◽  
Vol 33 (2) ◽  
pp. 214-218 ◽  
Author(s):  
Danúbia S. Fontana ◽  
Paulo Ricardo D. Rocha ◽  
Raquel A.S. Cruz ◽  
Letícya L. Lopes ◽  
Andréia L.T. Melo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetic Variability ◽  
Dna Sequencing ◽  
Canine Parvovirus ◽  
Phylogenetic Study ◽  
Vp2 Gene ◽  
Hemorrhagic Enteritis ◽  
Canine Parvovirus Type 2 ◽  
Representative Samples

Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-2c, displayed a low genetic variability although observed several amino acid substitution. These findings indicated that CPV-2c has been circulating in dogs from the Cuiabá Municipality in Midwestern Brazil.

Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

2016 ◽  
Vol 199 (4) ◽  
pp. 543-549 ◽  
Author(s):  
J. Figueiredo ◽  
C. Miranda ◽  
R. Souto ◽  
E. Silva ◽  
J. Fafetine ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Canine Parvovirus ◽  
Canine Parvovirus Type 2

Canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus

2008 ◽  
Vol 128 (1-2) ◽  
pp. 48-55 ◽  
Author(s):  
N. Spibey ◽  
N.M. Greenwood ◽  
D. Sutton ◽  
W.S.K. Chalmers ◽  
I. Tarpey
Keyword(s):  
Canine Parvovirus ◽  
Virulent Challenge ◽  
Canine Parvovirus Type 2

scholarly journals Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR

2007 ◽  
Vol 146 (1-2) ◽  
pp. 202-208 ◽  
Author(s):  
Gabriella Elia ◽  
Alessandra Cavalli ◽  
Costantina Desario ◽  
Eleonora Lorusso ◽  
Maria Stella Lucente ◽  
...  
Keyword(s):  
Real Time ◽  
Canine Parvovirus ◽  
Rt Pcr ◽  
Canine Parvovirus Type 2

scholarly journals Immunomodulatory effect of Bacillus toyonensis BCT-7112 supplementation in puppies vaccinated against canine parvovirus

2020 ◽  
Vol 40 (11) ◽  
pp. 898-902
Author(s):  
Helen C. Franz ◽  
Neida L. Conrad ◽  
Francisco D.S. Santos ◽  
Vitória S. Gonçalves ◽  
Renata N. Fonseca ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Canine Parvovirus ◽  
Immunomodulatory Effect ◽  
Mrna Transcription ◽  
Peripheral Blood Mononuclear ◽  
Specific Igg ◽  
Probiotic Microorganism ◽  
Canine Parvovirus Type 2 ◽  
Bacillus Toyonensis

ABSTRACT: Bacillus toyonensis is a probiotic microorganism that for decades has been used in animal nutrition around the world. The objective of this work was to evaluate the immunomodulatory effect of oral B. toyonensis supplementation in dogs vaccinated against canine parvovirus. Puppies were randomly selected and divided in two groups, one received B. toyonensis at a concentration of 2x108 viable spores per day and another group without supplementation was left as control. The puppies were vaccinated against canine parvovirus type 2. B. toyonensis supplementation was efficient in stimulating specific IgG for parvovirus with titers of 2, 3, and 2.5-fold higher than controls at 7, 21, and 35 pos-vaccination days respectively. Peripheral blood mononuclear cells (PBMCs) from dogs were cultured and stimulated with B. toyonensis DNA, vegetative cell and spores. The mRNA transcription of cytokines IL-4, IL-17, and IFN-γ up modulated by the stimuli. Thus, we conclude in this study that B. toyonensis supplementation may amplify the vaccine immune response against canine parvovirus.

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