scholarly journals Changes in Biomarkers after Initial Periodontal Treatment in Gingival Crevicular Fluid from Patients with Chronic Periodontitis Presenting with Drug-Induced Gingival Overgrowth

2016 ◽  
Vol 06 (02) ◽  
pp. 64-72 ◽  
Author(s):  
Taro Shimizu ◽  
Takehiko Kubota ◽  
Masanori Iwasaki ◽  
Toshiya Morozumi ◽  
Hiromasa Yoshie
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Periodontal Treatment ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Crevicular Fluid

scholarly journals Gingival crevicular fluid periostin levels in chronic periodontitis patients following nonsurgical periodontal treatment with low-level laser therapy

2016 ◽  
Vol 10 (04) ◽  
pp. 546-550 ◽  
Author(s):  
Dhanangchaayan Kumaresan ◽  
Aruna Balasundaram ◽  
Vanaja Krishna Naik ◽  
Deva Priya Appukuttan
Keyword(s):  
Laser Therapy ◽  
Chronic Periodontitis ◽  
Periodontal Ligament ◽  
Gingival Crevicular Fluid ◽  
Periodontal Treatment ◽  
Low Level Laser Therapy ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Crevicular Fluid

ABSTRACT Objective: Periostin is a matricellular protein highly expressed in periosteum, periodontal ligament and is essential for tissue integrity and maturation. It plays a role in collagen fibrillogenesis and is downregulated in periodontal disease. Biostimulation utilizing low-level laser therapy (LLLT) influences periodontal ligament fibroblast proliferation. This study was conducted with the objective of estimating periostin levels in chronic periodontitis (CP) patients following LLLT as an adjunct to root surface debridement (RSD). Materials and Methods: Thirty periodontally healthy participants (Group I) and sixty CP participants were recruited. Based on the therapeutic intervention, CP patients were allocated to either RSD (Group II) or to RSD with LLLT (Group III) group. Clinical parameters and gingival crevicular fluid (GCF) periostin levels were assessed at the baseline and at the 3rd month. Results: Periostin levels were significantly lower in CP patients when compared to healthy individuals at the baseline (P < 0.01). Following nonsurgical periodontal treatment (NSPT), periostin levels significantly increased in both Group II and III, when compared to baseline values (P < 0.001). Comparison of mean periostin levels between both the treatment groups showed a significant increase in LLLT group than RSD at the 3rd month (P < 0.05). Conclusion: Within the limitations of the present study, LLLT application was found to have additional benefits over RSD with respect to clinical periodontal parameters and GCF periostin levels. Moreover, periostin may be used as a possible biomarker to evaluate the outcome following NSPT.

scholarly journals Periodontal Treatment Downregulates Protease-Activated Receptor 2 in Human Gingival Crevicular Fluid Cells

2013 ◽  
Vol 81 (12) ◽  
pp. 4399-4407 ◽  
Author(s):  
Vanessa Tubero Euzebio Alves ◽  
Henrique Aparecido Bueno da Silva ◽  
Bruno Nunes de França ◽  
Rosangela Santos Eichler ◽  
Luciana Saraiva ◽  
...  
Keyword(s):  
Growth Factor ◽  
Inflammatory Mediators ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Periodontal Treatment ◽  
Positive Staining ◽  
Proinflammatory Mediators ◽  
Periodontal Inflammation ◽  
Crevicular Fluid ◽  
Protease Activated Receptor 2

ABSTRACTProtease-activated receptor 2 (PAR2) is implicated in the pathogenesis of chronic inflammatory diseases, including periodontitis; it can be activated by gingipain and produced byPorphyromonas gingivalisand by neutrophil protease 3 (P3). PAR2 activation plays a relevant role in inflammatory processes by inducing the release of important inflammatory mediators associated with periodontal breakdown. The effects of periodontal treatment on PAR2 expression and its association with levels of proinflammatory mediators and activating proteases were investigated in chronic periodontitis patients. Positive staining for PAR2 was observed in gingival crevicular fluid cells and was reflective of tissue destruction. Overexpression of PAR2 was positively associated with inflammatory clinical parameters and with the levels of interleukin-6 (IL-6), IL-8, tumor necrosis factor alpha, matrix metalloprotease 2 (MMP-2), MMP-8, hepatocyte growth factor, and vascular endothelial growth factor. Elevated levels of gingipain and P3 and decreased levels of dentilisin and the protease inhibitors secretory leukocyte protease inhibitor and elafin were also associated with PAR2 overexpression. Healthy periodontal sites from individuals with chronic periodontitis showed diminished expression of PAR2 mRNA and the PAR2 protein (P< 0.05). Furthermore, periodontal treatment resulted in decreased PAR2 expression and correlated with decreased expression of inflammatory mediators and activating proteases. We concluded that periodontal treatment resulted in decreased levels of proteases and that proinflammatory mediators are associated with decreased PAR2 expression, suggesting that PAR2 expression is influenced by the presence of periodontal infection and is not a constitutive characteristic favoring periodontal inflammation.

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