scholarly journals The <i>phka</i>1 deficient I/LnJ mouse exhibits endurance exercise deficiency with no compensatory changes in glycolytic gene expression

2013 ◽  
Vol 03 (02) ◽  
pp. 87-94
Author(s):  
Ashley M. Mefford ◽  
Claci C. Ayers ◽  
Naomi S. Rowland ◽  
Nancy A. Rice
Keyword(s):  
Gene Expression ◽  
Endurance Exercise ◽  
Glycolytic Gene

Aphid estrogen-related receptor controls glycolytic gene expression and fecundity

2021 ◽  
Vol 130 ◽  
pp. 103529
Author(s):  
Woo-Ram Park ◽  
Da Jung Lim ◽  
Hyunkyu Sang ◽  
Eunae Kim ◽  
Jae-Hak Moon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glycolytic Gene ◽  
Estrogen Related Receptor

The Effects of Endurance Exercise and Diet on Atherosclerosis in Young and Aged ApoE–/– and Wild-Type Mice

Gerontology ◽  
2018 ◽  
Vol 65 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Bojana Jakic ◽  
Mattias Carlsson ◽  
Maja Buszko ◽  
Giuseppe Cappellano ◽  
Christian Ploner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Endurance Exercise ◽  
Transforming Growth Factor ◽  
Physical Endurance ◽  
Heat Shock Protein 60 ◽  
Wild Type ◽  
Aortic Lesion ◽  
Tgf Β1 ◽  
Aortic Lesions

Background: Atherosclerosis is the leading cause of death worldwide. The disease development is by and large driven by old age and lifestyle factors, such as diet, physical activity, and smoking. In the present study, we have investigated the effect of exercise and diet on the development of atherosclerosis in young and aged mice. Objective: This study aimed at comparing multiple age-dependent factors that may influence atherosclerosis in a transgenic mouse model. Methods: Young (14 weeks) and aged (49–52 weeks) C57BL/6 wild-type (WT) and atherosclerosis-prone ApoE–/– mice were subjected to physical endurance exercise on a treadmill, with or without a high-fat diet. Five weeks later, the frequencies of regulatory T cells (TREGs) in lymph nodes were assessed by flow cytometry, plasmatic cytokines (interleukin [IL]-1β, IL-6, IL-10, IL-17, interferon-γ, tumor necrosis factor-α, and transforming growth factor [TGF]-β1) levels were determined by Luminex assay. Lipids (cholesterol and triglycerides) and anti-heat shock protein 60 (HSP60) autoantibodies were measured by ELISA. Aortic lesion sizes were assessed by en face imaging. Microarray analysis and qPCR of skeletal muscle gene expression were also performed. Results: Exercise leads to a reduction of aortic lesions in young ApoE–/– and aged WT mice independent of diet. In most groups, this reduction was followed by an increased proportion of TREGs and TGF-β1 levels. Moreover, gene expression analysis showed that exercise seems to affect the AMPK signaling pathway. In particular, PGC-1α1 mRNA was induced in aged WT mice, whereas it was reduced in young ApoE–/– mice. In addition, GSEA analysis showed a marked reduction in the insulin signaling pathway in aged ApoE–/– mice. Conclusion: Practicing endurance exercise seems to be enough for reducing early aortic lesion formation, independent of diet. However, this was only true in mice with smaller aortic lesions, since mice with large, advanced, complicated atherosclerotic plaques did not show any reduction in lesion size with exercise training.

Effect of Endurance Exercise Training on Liver Gene Expression in Male and Female Mice

2020 ◽  
Author(s):  
Luma Melo ◽  
Karen Tilmant ◽  
Amit Hagar ◽  
JAMES E KLAUNIG
Keyword(s):  
Gene Expression ◽  
Metabolic Disease ◽  
Endurance Exercise ◽  
Metabolic Diseases ◽  
Molecular Pathways ◽  
Hepatic Gene Expression ◽  
Chronic Exercise ◽  
Male And Female ◽  
Female Mice ◽  
Liver Gene

Chronic endurance exercise is a therapeutic strategy in the treatment of many chronic diseases in humans, including the prevention and treatment of metabolic diseases such as diabetes mellitus. Metabolic, cardiorespiratory and endocrine pathways targeted by chronic endurance exercise have been identified. In the liver however, the cellular and molecular pathways that are modified by exercise and have preventive or therapeutic relevance to metabolic disease remain unresolved. The mouse model used in the current study allows for the quantification of a human-relevant exercise “dosage”. In this study we show hepatic gene expression differences between sedentary female and sedentary male mice, and that chronic exercise modifies the transcription of hepatic genes related to metabolic disease and steatosis in both male and female mice. Chronic exercise induces molecular pathways involved in glucose tolerance, glycolysis and gluconeogenesis while producing a decrease in pathways related to insulin resistance, steatosis, fibrosis, and inflammation. Given these findings, this mouse exercise model has potential to dissect the cellular and molecular hepatic changes following chronic exercise with application to understanding the role that chronic exercise plays in preventing human diseases. Novelty Bullets: • Exercise modifies the hepatic gene expression and hepatic pathways related to metabolic disease in male and female mice. • Gender differences were seen in hepatic gene expression between sedentary and exercised mice. • The mouse exercise model used in this study allows for application and evaluation of exercise effects in human disease

Time course-dependent changes in the transcriptome of human skeletal muscle during recovery from endurance exercise: from inflammation to adaptive remodeling

2014 ◽  
Vol 116 (3) ◽  
pp. 274-287 ◽  
Author(s):  
Oliver Neubauer ◽  
Surendran Sabapathy ◽  
Kevin J. Ashton ◽  
Ben Desbrow ◽  
Jonathan M. Peake ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Endurance Exercise ◽  
Time Course ◽  
Acute Stress ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Matrix Remodeling ◽  
Molecular Signatures ◽  
Gene Sets

Reprogramming of gene expression is fundamental for skeletal muscle adaptations in response to endurance exercise. This study investigated the time course-dependent changes in the muscular transcriptome after an endurance exercise trial consisting of 1 h of intense cycling immediately followed by 1 h of intense running. Skeletal muscle samples were taken at baseline, 3 h, 48 h, and 96 h postexercise from eight healthy, endurance-trained men. RNA was extracted from muscle. Differential gene expression was evaluated using Illumina microarrays and validated with qPCR. Gene set enrichment analysis identified enriched molecular signatures chosen from the Molecular Signatures Database. Three hours postexercise, 102 gene sets were upregulated [family wise error rate (FWER), P < 0.05], including groups of genes related with leukocyte migration, immune and chaperone activation, and cyclic AMP responsive element binding protein (CREB) 1 signaling. Forty-eight hours postexercise, among 19 enriched gene sets (FWER, P < 0.05), two gene sets related to actin cytoskeleton remodeling were upregulated. Ninety-six hours postexercise, 83 gene sets were enriched (FWER, P < 0.05), 80 of which were upregulated, including gene groups related to chemokine signaling, cell stress management, and extracellular matrix remodeling. These data provide comprehensive insights into the molecular pathways involved in acute stress, recovery, and adaptive muscular responses to endurance exercise. The novel 96 h postexercise transcriptome indicates substantial transcriptional activity potentially associated with the prolonged presence of leukocytes in the muscles. This suggests that muscular recovery, from a transcriptional perspective, is incomplete 96 h after endurance exercise involving muscle damage.

PS7 - 38. Effects of acute endurance exercise on gene expression in skeletal muscle

2011 ◽  
Vol 9 (3) ◽  
pp. 117-117
Author(s):  
Milène Catoire ◽  
Marco Mensink ◽  
Patrick Schrauwen ◽  
Sander Kersten
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Endurance Exercise

scholarly journals Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1496 ◽  
Author(s):  
Takaki Tominaga ◽  
Sihui Ma ◽  
Kumiko Saitou ◽  
Katsuhiko Suzuki
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Endurance Exercise ◽  
Treadmill Running ◽  
Macrophage Infiltration ◽  
Special Focus ◽  
Water Ingestion ◽  
Glucose Ingestion ◽  
Exercise Induced

Background: Carbohydrate (CHO) supplementation during exercise attenuates exercise-induced increases in plasma Interleukin (IL)-6 concentration. However, the effects of CHO supplementation on muscle IL-6 production during endurance exercise is controversial. The purpose of this study was to investigate the effects of CHO supplementation on muscle IL-6 production during endurance exercise with a special focus on the IL-6 producing cells. Methods: C57BL/6J mice were divided into three groups—sedentary with water ingestion group as the control (Con; n = 10), exercise with water ingestion group (Ex; n = 10), and exercise with 6% glucose ingestion group (Ex + glucose; n = 10). The Ex and Ex + glucose groups completed 3 h of treadmill running (24 m/min, 7% incline) and were sacrificed immediately after exercise. Results: The exercise-induced increases of plasma IL-6 concentration and gastrocnemius IL-6 gene expression were attenuated by glucose ingestion. However, the increases of soleus IL-6 gene expression and gastrocnemius and soleus IL-6 protein expression were not attenuated by glucose ingestion. Furthermore, we observed that macrophages that infiltrated muscle produce IL-6 and glucose ingestion attenuated the infiltration of IL-6-producing macrophages. Conclusion: This study revealed that infiltrating macrophages may be one type of IL-6-producing cells during endurance exercise, and the infiltration of these cells in muscle was attenuated by glucose ingestion. However, the effects of glucose ingestion on muscle IL-6 production were limited.

scholarly journals Pronounced Effects of Acute Endurance Exercise on Gene Expression in Resting and Exercising Human Skeletal Muscle

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e51066 ◽  
Author(s):  
Milène Catoire ◽  
Marco Mensink ◽  
Mark V. Boekschoten ◽  
Roland Hangelbroek ◽  
Michael Müller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Endurance Exercise ◽  
Human Skeletal Muscle

Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites

1990 ◽  
Vol 10 (2) ◽  
pp. 859-862
Author(s):  
G M Santangelo ◽  
J Tornow
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Dna Binding ◽  
Binding Sites ◽  
Binding Activity ◽  
Heterologous Gene ◽  
Dna Binding Activity ◽  
Glycolytic Gene

Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UASRPG elements). We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity.

scholarly journals PBMCs express a transcriptome signature predictor of oxygen uptake responsiveness to endurance exercise training in men

2015 ◽  
Vol 47 (2) ◽  
pp. 13-23 ◽  
Author(s):  
Rodrigo Gonçalves Dias ◽  
Michelle Sabrina Moreira Silva ◽  
Nubia Esteban Duarte ◽  
Wladimir Bolani ◽  
Cleber Renê Alves ◽  
...  
Keyword(s):  
Gene Expression ◽  
Exercise Training ◽  
Oxygen Uptake ◽  
Endurance Training ◽  
Endurance Exercise ◽  
Peripheral Blood ◽  
Functional Enrichment ◽  
Total Rna ◽  
Transcriptional Signature ◽  
Endurance Exercise Training

Peripheral blood cells are an accessible environment in which to visualize exercise-induced alterations in global gene expression patterns. We aimed to identify a peripheral blood mononuclear cell (PBMC) signature represented by alterations in gene expression, in response to a standardized endurance exercise training protocol. In addition, we searched for molecular classifiers of the variability in oxygen uptake (V̇o2). Healthy untrained policemen recruits ( n = 13, 25 ± 3 yr) were selected. Peak V̇o2 (measured by cardiopulmonary exercise testing) and total RNA from PBMCs were obtained before and after 18 wk of running endurance training (3 times/wk, 60 min). Total RNA was used for whole genome expression analysis using Affymetrix GeneChip Human Gene 1.0 ST. Data were normalized by the robust multiarray average algorithm. Principal component analysis was used to perform correlations between baseline gene expression and V̇o2peak. A set of 211 transcripts was differentially expressed (ANOVA, P < 0.05 and fold change > 1.3). Functional enrichment analysis revealed that transcripts were mainly related to immune function, cell cycle processes, development, and growth. Baseline expression of 98 and 53 transcripts was associated with the absolute and relative V̇o2peak response, respectively, with a strong correlation ( r > 0.75, P < 0.01), and this panel was able to classify the 13 individuals according to their potential to improve oxygen uptake. A subset of 10 transcripts represented these signatures to a similar extent. PBMCs reveal a transcriptional signature responsive to endurance training. Additionally, a baseline transcriptional signature was associated with changes in V̇o2peak. Results might illustrate the possibility of obtaining molecular classifiers of endurance capacity changes through a minimally invasive blood sampling procedure.

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