scholarly journals Comparison of the Rose Bengal Plate and the Complement Fixation Tests with the Tube Agglutination Test for Diagnosis of Human Brucellosis

2017 ◽  
Vol 07 (03) ◽  
pp. 73-82 ◽  
Author(s):  
Ying-Hock Teng ◽  
Jung-Jing Teng ◽  
Samantha Chao ◽  
Hsu Chao ◽  
Suryakant D. Waghela
Keyword(s):  
Rose Bengal ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
The Rose

scholarly journals Comparison of the results of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 365-371 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Comparison Of The Results ◽  
The Rose ◽  
Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

Seroprevalence of human brucellosis in rural endemic areas in eastern Turkey

2008 ◽  
Vol 38 (1) ◽  
pp. 42-43 ◽  
Author(s):  
Serhat Vancelik ◽  
Asuman Guraksin ◽  
Ahmet Ayyildiz
Keyword(s):  
Public Health ◽  
Rose Bengal ◽  
Health Problem ◽  
Public Health Problem ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
Community Based ◽  
Cross Sectional ◽  
Rose Bengal Test ◽  
The Rose

In this cross-sectional, community-based study, sera were obtained from 573 subjects. Brucella seropositivity was detected in 5.4% according to the standard tube agglutination test, rising to 11.9% when the Rose-Bengal test was used. Brucellosis is a serious public health problem in eastern Tur key.

scholarly journals The Rose Bengal Test in Human Brucellosis: A Neglected Test for the Diagnosis of a Neglected Disease

2011 ◽  
Vol 5 (4) ◽  
pp. e950 ◽  
Author(s):  
Ramón Díaz ◽  
Aurora Casanova ◽  
Javier Ariza ◽  
Ignacio Moriyón
Keyword(s):  
Rose Bengal ◽  
Human Brucellosis ◽  
Neglected Disease ◽  
Rose Bengal Test ◽  
The Rose

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

The rose bengal plate agglutination test in the diagnosis of brucellosis

1969 ◽  
Vol 85 (23) ◽  
pp. 636-641 ◽  
Author(s):  
WJ Morgan ◽  
DJ MacKinnon ◽  
JR Lawson ◽  
GA Cullen
Keyword(s):  
Rose Bengal ◽  
Agglutination Test ◽  
The Rose

scholarly journals Evaluation of Seven Tests for Diagnosis of Human Brucellosis in an Area Where the Disease Is Endemic

2008 ◽  
Vol 15 (6) ◽  
pp. 1031-1033 ◽  
Author(s):  
M. Concepción Gómez ◽  
José A. Nieto ◽  
Carmen Rosa ◽  
Paloma Geijo ◽  
M. Ángeles Escribano ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Immunoglobulin G ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
Coombs Test ◽  
Specific Antibodies ◽  
Enzyme Immunoassays ◽  
Serologic Tests ◽  
Microagglutination Test ◽  
Conventional Tests

ABSTRACT The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.

scholarly journals Prevalence of antibodies to Brucella spp. in cattle, sheep, goats, horses and camels in the State of Eritrea; influence of husbandry systems

2000 ◽  
Vol 125 (2) ◽  
pp. 447-453 ◽  
Author(s):  
M. K. OMER ◽  
E. SKJERVE ◽  
G. HOLSTAD ◽  
Z. WOLDEHIWET ◽  
A. P. MACMILLAN
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Dairy Herds ◽  
Serological Evidence ◽  
Vaccination Site ◽  
Mixed Crop ◽  
Husbandry System ◽  
The Rose ◽  
Livestock System

Samples from 2427 cattle, 661 goats, 104 sheep, 98 camels and 82 horses were screened for brucella infections by the Rose Bengal Test and positive reactors confirmed by the complement fixation test. In cattle, the highest individual seroprevalence was in dairy herds kept under the intensive husbandry system, with an individual prevalence of 8·2% and unit (herd) seroprevalence of 35·9%. This was followed by the pastoral husbandry system in the Western Lowlands with 5·0% individual but a higher unit (vaccination site) prevalence of 46·1%. The lowest was in the mixed crop-livestock system in the Southern Highlands with individual 0·3% and unit (village) prevalence of 2·4%. In sheep and goats, no positive animals were detected in the mixed crop-livestock areas. In the Eastern Lowlands individual prevalences of 3·8% (goats) and 1·4% (sheep) and unit prevalence of 33·3% (goats) and 16·7% were found, while 14·3% of individual goats and 56·3% of the units in the Western Lowlands were positive. No positive horses were found. The present study documents the first serological evidence of Brucella spp. infection in camels (3·1%) in Eritrea.

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