scholarly journals Expression of a Testis-Specific Nuclear Protein, TRA98, in Mouse Testis during Spermatogenesis. A Quantitative and Qualitative Immunoelectron Microscopy (IEM) Analysis

2011 ◽  
Vol 01 (01) ◽  
pp. 11-20 ◽  
Author(s):  
Natsuki Inoue
Keyword(s):  
Immunoelectron Microscopy ◽  
Nuclear Protein ◽  
Mouse Testis ◽  
Specific Nuclear Protein

scholarly journals High-affinity binding site for a specific nuclear protein in the human IgM gene

Nature ◽  
1985 ◽  
Vol 315 (6016) ◽  
pp. 254-254
Author(s):  
L. Hennighausen ◽  
U. Siebenlist ◽  
D. Danner ◽  
P. Leder ◽  
D. Rawlins ◽  
...  
Keyword(s):  
Binding Site ◽  
Nuclear Protein ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
High Affinity Binding Site ◽  
Specific Nuclear Protein

Arabidopsis EED1 encoding a plant-specific nuclear protein is essential for early embryogenesis

2020 ◽  
Vol 47 (1) ◽  
pp. 61-64
Author(s):  
Mai Yang ◽  
Chun Yan ◽  
Megan Griffith ◽  
Jinping Zhao ◽  
Yongbiao Zhang ◽  
...  
Keyword(s):  
Nuclear Protein ◽  
Early Embryogenesis ◽  
Specific Nuclear Protein

scholarly journals HANP1/H1T2, a Novel Histone H1-Like Protein Involved in Nuclear Formation and Sperm Fertility

2005 ◽  
Vol 25 (16) ◽  
pp. 7107-7119 ◽  
Author(s):  
Hiromitsu Tanaka ◽  
Naoko Iguchi ◽  
Ayako Isotani ◽  
Kouichi Kitamura ◽  
Yoshiro Toyama ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Nuclear Protein ◽  
Physiological Role ◽  
Histone H1 ◽  
Vitro Fertilization ◽  
Rich Domain ◽  
And Function ◽  
Specific Nuclear Protein

ABSTRACT We cloned a testis-specific cDNA from mice that encodes a histone H1-like, haploid germ cell-specific nuclear protein designated HANP1/H1T2. The HANP1/H1T2 protein was specifically localized to the nuclei of murine spermatids during differentiation steps 5 to 13 but not to the nuclei of mature sperm. HANP1/H1T2 contains an arginine-serine-rich domain and an ATP/GTP binding site, and it binds to DNA, ATP, and protamine. To investigate the physiological role of HANP1/H1T2, we generated Hanp1/H1T2-disrupted mutant mice. Homozygous Hanp1/H1T2 mutant males were infertile, but females were fertile. Although a substantial number of sperm were recovered from the epididymides, their shape and function were abnormal. During sperm morphogenesis, the formation of nuclei was disturbed and protamine-1 and -2 were only weakly detectable in the nuclei. The chromatin packaging was aberrant, as demonstrated by electron microscopy and biochemical analysis. The mutant sperm exhibited deficient motility and were not competent to fertilize eggs under in vitro fertilization conditions; however, they were capable of fertilizing eggs via intracytoplasmic sperm injection that resulted in the birth of healthy progeny. Thus, we found that HANP1/H1T2 is essential for nuclear formation in functional spermatozoa and is specifically involved in the replacement of histones with protamines during spermiogenesis. At the time of submission of the manuscript, we found an independent publication by Martianov et al. (I. Martianov, S. Brancorsini, R. Catena, A. Gansmuller, N. Kotaja, M. Parvinen, P. Sassone-Corsi, and I. Davidson, Proc. Natl. Acad. Sci. USA 102:2808-2813, 2005) that reported similar results.

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