scholarly journals <i>Trypanosoma evansi</i>: A Qualitative and Quantitative Ultrastructural Analysis of the Spleen during Experimental Murine Infections

2020 ◽  
Vol 08 (03) ◽  
pp. 31-42
Author(s):  
Lucia Proietti d’Empaire ◽  
Félix Tejero ◽  
Hector J. Finol ◽  
Pedro M. Aso ◽  
Antonio Roschman-Gonzalez
2010 ◽  
Vol 12 (6) ◽  
pp. 844-861 ◽  
Author(s):  
Mustafa Ulasli ◽  
Monique H. Verheije ◽  
Cornelis A. M. De Haan ◽  
Fulvio Reggiori

2018 ◽  
Vol 38 (3) ◽  
pp. 279 ◽  
Author(s):  
Felix Tejero ◽  
Lourdes Lorena Arias-Mota ◽  
Antonio Roschman-González ◽  
Pedro María Aso ◽  
Héctor José Finol

2020 ◽  
Vol 38 (5) ◽  
pp. 1271-1280
Author(s):  
Héctor J Finol ◽  
Estefanie García ◽  
Roschman González ◽  
Elda E Sanchez ◽  
Alexis Rodríguez-Acosta

Author(s):  
R.L. Price ◽  
T.K. Borg ◽  
L. Terracio ◽  
M. Nakagawa

Little is known about the temporal expression of extracellular matrix components (ECM) and its receptors during development of the heart. Recent reports have shown that ECM components undergo both qualitative and quantitative changes during development, and it is believed that ECM components are important in the regulation of cell migration and cell:cell and cell:ECM recognition and adhesion.Integrins are transmembrane glycoproteins which bind several ECM components on their external face and cytoskeletal elements on the cytoplasmic face. Laminin is a basement membrane component which has been recognized as an important site for cell adhesion. Both the integrins and laminin are expressed early in development and continue to be expressed in the adult heart. With their documented roles in cell recognition, and cell:cell and cell:ECM migration and adhesion these proteins appear to be important components in development of the heart, and their temporal expression may play a pivotal role in morphogenesis and myofibrillogenesis of the heart.


Author(s):  
I. Brent Heath

Detailed ultrastructural analysis of fungal mitotic systems and cytoplasmic microtubules might be expected to contribute to a number of areas of general interest in addition to the direct application to the organisms of study. These areas include possibly fundamental general mechanisms of mitosis; evolution of mitosis; phylogeny of organisms; mechanisms of organelle motility and positioning; characterization of cellular aspects of microtubule properties and polymerization control features. This communication is intended to outline our current research results relating to selected parts of the above questions.Mitosis in the oomycetes Saprolegnia and Thraustotheca has been described previously. These papers described simple kinetochores and showed that the kineto- chores could probably be used as markers for the poorly defined chromosomes. Kineto- chore counts from serially sectioned prophase mitotic nuclei show that kinetochore replication precedes centriole replication to yield a single hemispherical array containing approximately the 4 n number of kinetochore microtubules diverging from the centriole associated "pocket" region of the nuclear envelope (Fig. 1).


Author(s):  
Jerrold L. Abraham

Inorganic particulate material of diverse types is present in the ambient and occupational environment, and exposure to such materials is a well recognized cause of some lung disease. To investigate the interaction of inhaled inorganic particulates with the lung it is necessary to obtain quantitative information on the particulate burden of lung tissue in a wide variety of situations. The vast majority of diagnostic and experimental tissue samples (biopsies and autopsies) are fixed with formaldehyde solutions, dehydrated with organic solvents and embedded in paraffin wax. Over the past 16 years, I have attempted to obtain maximal analytical use of such tissue with minimal preparative steps. Unique diagnostic and research data result from both qualitative and quantitative analyses of sections. Most of the data has been related to inhaled inorganic particulates in lungs, but the basic methods are applicable to any tissues. The preparations are primarily designed for SEM use, but they are stable for storage and transport to other laboratories and several other instruments (e.g., for SIMS techniques).


Author(s):  
Gerald Rupp

The marine protozoan Allogromia sp, strain NF Lee extends an elaborate reticulopodial network (RN) which contains an elongate microtubule-(MT)-based cytoskeleton. The MTs are located primarily within cytoplasmic fibrils which are visible by light microscopy (LM) in highly flattened or “two dimensionalized” reticulopodia. It was shown previously that allogromiid RNs withdraw in response to hypertonic Mg2+-seawater. An ultrastructural analysis of this phenomenon indicated that large patches of paracrystalline (PC) material, composed of helical filament aggregates, form concomitant with a decrease in MT number. Similar large patches of PC aggregates are also found in juvenile Allogromia before they extend a RN, which disappear during RN formation. Finally, PC aggregates are occasionally seen near microtubules in normal untreated RNs. Thus there is circumstantial evidence to propose that PC aggregates in Allogromia represent an intermediate form of tubulin; however, more definitive biochemical or immunocytochemical data is not available.


VASA ◽  
2012 ◽  
Vol 41 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Hoerth ◽  
Kundi ◽  
Katzenschlager ◽  
Hirschl

Background: Nailfold capillaroscopy (NVC) is a diagnostic tool particularly useful in the differential diagnosis of rheumatic and connective tissue diseases. Although successfully applied since many years, little is known about prevalence and distribution of NVC changes in healthy individuals. Probands and methods: NVC was performed in 120 individuals (57 men and 63 women; age 18 to 70 years) randomly selected according to predefined age and sex strata. Diseases associated with NVC changes were excluded. The nailfolds of eight fingers were assessed according to standardized procedures. A scoring system was developed based on the distribution of the number of morphologically deviating capillaries, microhaemorrhages, and capillary density. Results: Only 18 individuals (15 %) had no deviation in morphology, haemorrhages, or capillary density on any finger. Overall 67 % had morphological changes, 48 % had microhaemorrhages, and 40 % of volunteers below 40 years of age and 18 % above age 40 had less than 8 capillaries/mm. Among morphological changes tortous (43 %), ramified (47 %), and bushy capillaries (27 %) were the most frequently altered capillary types. A semiquantitative scoring system was developed in such a way that a score above 1 indicates an extreme position (above the 90th percentile) in the distribution of scores among healthy individuals. Conclusions: Altered capillaries occur frequently among healthy individuals and should be interpreted as normal unless a suspicious increase in their frequency is determined by reference to the scoring system. Megacapillaries and diffuse loss of capillaries were not found and seem to be of specific diagnostic value.


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