scholarly journals Identification of Bulge Stem Cells in Mouse and Human Hair Follicles

2018 ◽  
Vol 06 (03) ◽  
pp. 19-29
Author(s):  
Bélgica J. Molina ◽  
Elda Giansante ◽  
Héctor J. Finol
Keyword(s):  
Stem Cells ◽  
Human Hair ◽  
Hair Follicles

scholarly journals Stem cells from human hair follicles: first mechanical isolation for immediate autologous clinical use in androgenetic alopecia and hair loss

2017 ◽  
Vol 4 (7) ◽  
pp. 58-58 ◽  
Author(s):  
Pietro Gentile ◽  
Maria G. Scioli ◽  
Alessandra Bielli ◽  
Augusto Orlandi ◽  
Valerio Cervelli
Keyword(s):  
Stem Cells ◽  
Hair Loss ◽  
Human Hair ◽  
Hair Follicles ◽  
Androgenetic Alopecia ◽  
Clinical Use ◽  
Mechanical Isolation

scholarly journals Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles

2006 ◽  
Vol 168 (6) ◽  
pp. 1879-1888 ◽  
Author(s):  
Hong Yu ◽  
Dong Fang ◽  
Suresh M. Kumar ◽  
Ling Li ◽  
Thiennga K. Nguyen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Hair ◽  
Adult Stem Cells ◽  
Hair Follicles

scholarly journals Induced pluripotent stem cells from human hair follicle keratinocytes as a potential source for in vitro hair follicle cloning

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2695 ◽  
Author(s):  
Sheng Jye Lim ◽  
Shu Cheow Ho ◽  
Pooi Ling Mok ◽  
Kian Lee Tan ◽  
Alan H.K. Ong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Hair Follicle ◽  
Pluripotent Stem Cells ◽  
Human Hair ◽  
Hair Follicles ◽  
Reverse Transcription Pcr ◽  
Pluripotency Markers ◽  
Induced Pluripotent

Background Human hair follicles are important for the renewal of new hairs and their development. The generation of induced pluripotent stem cells (iPSCs) from hair follicles is easy due to its accessibility and availability. The pluripotent cells derived from hair follicles not only have a higher tendency to re-differentiate into hair follicles, but are also more suited for growth in hair scalp tissue microenvironment. Methods In this study, human hair follicular keratinocytes were used to generate iPSCs, which were then further differentiated in vitro into keratinocytes. The derived iPSCs were characterised by using immunofluorescence staining, flow cytometry, and reverse-transcription PCR to check for its pluripotency markers expression. Results The iPSC clones expressed pluripotency markers such as TRA-1-60, TRA-1-81, SSEA4, OCT4, SOX2, NANOG, LEFTY, and GABRB. The well-formed three germ layers were observed during differentiation using iPSCs derived from hair follicles. The successful formation of keratioctyes from iPSCs was confirmed by the expression of cytokeratin 14 marker. Discussion Hair follicles represent a valuable keratinocytes source for in vitro hair cloning for use in treating hair balding or grafting in burn patients. Our significant findings in this report proved that hair follicles could be used to produce pluripotent stem cells and suggested that the genetic and micro-environmental elements of hair follicles might trigger higher and more efficient hair follicles re-differentiation.

scholarly journals LARGE-SCALE EXPANSION AND CHARACTERIZATION OF HUMAN ADULT NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS FROM HAIR FOLLICLE FOR REGENERATIVE MEDICINE APPLICATIONS

2017 ◽  
Vol 39 (3) ◽  
pp. 171-180 ◽  
Author(s):  
R G Vasyliev ◽  
A E Rodnichenko ◽  
O S Gubar ◽  
A V Zlatska ◽  
I M Gordiienko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Hair Follicle ◽  
Human Hair ◽  
Large Scale ◽  
Hair Follicles ◽  
Human Hair Follicle ◽  
Self Renewal ◽  
Multipotent Stem Cells ◽  
Clonogenic Potential

Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2+Sox10+Nestin+CD73+CD90+CD105+CD140a+CD 140b+CD146+CD166+CD271+CD349+ CD34-CD45-CD56-HLA-DR-, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice.

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