scholarly journals Optimization of Liquid Ammonia Treatment for Enzymatic Hydrolysis of Miscanthus sinensis Anderss

2015 ◽  
Vol 03 (07) ◽  
pp. 26-32 ◽  
Author(s):  
Liping Zhang ◽  
Hehuan Peng ◽  
Qinling Yu ◽  
Ying Zhang ◽  
Zhijun Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Liquid Ammonia ◽  
Miscanthus Sinensis ◽  
Ammonia Treatment ◽  
Hydrolysis Of

Bioethanol Production by Optimal Enzymatic Hydrolysis of Pretreated Miscanthus sinensis var. purpurascens

2015 ◽  
Vol 49 (4) ◽  
pp. 135-145
Author(s):  
Hak Gon Kim ◽  
◽  
Hyun Jin Song ◽  
Dong Jin Park ◽  
Woo Hyung Yang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Miscanthus Sinensis ◽  
Bioethanol Production ◽  
Hydrolysis Of

scholarly journals Effects of milling combined with extremely low-liquid ammonia (ELLA) pretreatment to enhance enzymatic hydrolysis of corn stover

2021 ◽  
pp. 105-114
Keyword(s):  
Energy Consumption ◽  
Enzymatic Hydrolysis ◽  
Ball Milling ◽  
Large Scale ◽  
Liquid Ammonia ◽  
Milling Time ◽  
Lignocellulosic Feedstock ◽  
Pretreatment Time ◽  
Hydrolysis Of ◽  
Ball Milling Time

Lignocellulosic biomass is one of the largest carbohydrate sources and has huge potential for biofuels production. However, the problem with lignocellulosic feedstock is that it has useful sugars locked in by lignin, hemicellulose, and cellulose. Some kind of pretreatment; therefore is needed to make carbohydrate accessible which later can be fermented to produce ethanol. The results from this research indicated that the yields of glucan (93%) and xylan (82.8%) were improved by using milling combined with ELLA pretreatment. The optimal enzymatic hydrolysis efficiencies were obtained under 10 min for ball milling time, pretreatment at 1 h, temperature at 150°C, S/L = 0.5 and ammonia loading at 0.25 g-NH3/g-biomass. This method reduced the pretreatment time and short milling time and thus has potential of reducing the energy consumption and promising the application in the large scale.

Microwave-assisted enzymatic hydrolysis of starch

2009 ◽  
Author(s):  
Marcin Lukasiewicz ◽  
Anna Osowiec ◽  
Magdalena Marciniak
Keyword(s):  
Enzymatic Hydrolysis ◽  
Microwave Assisted ◽  
Hydrolysis Of

Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.  

2018 ◽  
Author(s):  
Ángel Batallas ◽  
Erenio González ◽  
Carmen Salvador ◽  
Jonathan Villavicencio ◽  
Humberto González Gavilánez ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrolysis Of

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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