scholarly journals The Opioid Growth Factor Inhibits Established Ovarian Cancer in Nude Mice and Can Be Combined with Taxol or Cisplatin to Enhance Growth Inhibition

2011 ◽  
Vol 02 (02) ◽  
pp. 110-124 ◽  
Author(s):  
Renee N. Donahue ◽  
Ian S. Zagon ◽  
Patricia J. McLaughlin
Keyword(s):  
Ovarian Cancer ◽  
Growth Factor ◽  
Growth Inhibition ◽  
Nude Mice ◽  
Opioid Growth Factor

Cell proliferation of human ovarian cancer is regulated by the opioid growth factor-opioid growth factor receptor axis

2009 ◽  
Vol 296 (6) ◽  
pp. R1716-R1725 ◽  
Author(s):  
Renee N. Donahue ◽  
Patricia J. McLaughlin ◽  
Ian S. Zagon
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Growth Factor ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Cancer Cell Lines ◽  
Human Ovarian Cancer ◽  
Opioid Growth Factor ◽  
Ovarian Cancer Cell Lines

Ovarian cancer is the leading cause of death from gynecological malignancies. Understanding the biology of these tumors, as well as treatment modalities, has been challenging. The opioid growth factor (OGF; [Met5]-enkephalin) and the OGF receptor (OGFr) form an endogenous growth-regulating pathway in homeostasis and neoplasia. In this investigation, we examined the relationship of the OGF-OGFr axis to ovarian cancer, and defined its presence, function, and mechanisms. Using OVCAR-3 and SKOV-3 ovarian cancer cell lines, we found that OGF and OGFr were present and functional. Exogenous OGF was observed to have a dose-dependent, serum-independent, reversible, and receptor-mediated inhibitory action on cell proliferation that was dependent on RNA and protein synthesis. The repressive effect of OGF on cell proliferation also was observed in SW626, CAOV-3, and HEY ovarian cancer cell lines. Endogenous OGF was found to be constitutively produced and tonically active on cell replicative activities, with neutralization of this peptide accelerating cell proliferation. Silencing of OGFr using siRNA technology stimulated cell replication, documenting its integral role. The mechanism of OGF-OGFr action on DNA synthesis was related to the cyclin-dependent kinase inhibitory pathway because knockdown of p16 or p21 in OVCAR-3 cells, and p21 in SKOV-3 cells, eliminated OGF's inhibitory effect on growth. These data are the first to report that the OGF-OGFr system is a native biological regulator of cell proliferation in human ovarian cancer. This information will be important in designing treatment strategies for this deadly disease.

Expression of the opioid growth factor–opioid growth factor receptor axis in human ovarian cancer

2012 ◽  
Vol 124 (2) ◽  
pp. 319-324 ◽  
Author(s):  
James Fanning ◽  
Carrie A. Hossler ◽  
Joshua P. Kesterson ◽  
Renee N. Donahue ◽  
Patricia J. McLaughlin ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Human Ovarian Cancer ◽  
Opioid Growth Factor

Up‐regulation of insulin‐like growth factor binding protein‐3 by apigenin leads to growth inhibition and apoptosis of 22Rv1 xenograft in athymic nude mice

2005 ◽  
Vol 19 (14) ◽  
pp. 2042-2044 ◽  
Author(s):  
Sanjeev Shukla ◽  
Anil Mishra ◽  
Pingfu Fu ◽  
Gregory T. Maclennan ◽  
Martin I. Resnick ◽  
...  
Keyword(s):  
Growth Factor ◽  
Growth Inhibition ◽  
Nude Mice ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Athymic Nude Mice ◽  
Factor Binding

scholarly journals Growth inhibition of thyroid follicular cell-derived cancers by the opioid growth factor (OGF) - opioid growth factor receptor (OGFr) axis

BMC Cancer ◽  
2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Patricia J McLaughlin ◽  
Ian S Zagon ◽  
Sunny S Park ◽  
Andrea Conway ◽  
Renee N Donahue ◽  
...  
Keyword(s):  
Growth Factor ◽  
Growth Inhibition ◽  
Growth Factor Receptor ◽  
Follicular Cell ◽  
Thyroid Follicular Cell ◽  
Opioid Growth Factor
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