scholarly journals <i>In Vitro</i> Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

2012 ◽  
Vol 02 (02) ◽  
pp. 45-50 ◽  
Author(s):  
Maria Helena Madruga Lima-Ribeiro ◽  
Ralph Santos-Oliveira ◽  
Mauricélia Firmino de Santana ◽  
Terezinha de Jesus Andreoli Pinto ◽  
Irene Satiko Kikuchi ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Microbiological Control ◽  
Cratylia Mollis

Decontamination of Mikania glomerata Leaves by Gamma Irradiation: Coumarin Determination by HPLC-DAD, Microbiological Control and Genotoxicological Studies

Planta Medica ◽  
2017 ◽  
Vol 84 (01) ◽  
pp. 65-72
Author(s):  
Marcos Gouvêa ◽  
Samara Ferreira-Machado ◽  
Thalita da Silva ◽  
Julien Lima ◽  
Cláudio Lau ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Raw Material ◽  
In Vivo Models ◽  
Production Chain ◽  
Microbiological Control ◽  
Mikania Glomerata

AbstractGamma irradiation as a decontaminating physical agent could be an important tool in the production chain of herbal medicines by improving the microbiological quality of raw materials and the safety of final products. This study was undertaken to investigate the genotoxic potential and eventual chemical modifications of a batch of Mikania glomerata raw material decontaminated by different doses of gamma irradiation (2.0, 3.5, and 5.0 kGy), using a cesium-137 source. DNA damage was assessed in vitro by agarose gel electrophoresis in regard to double-chain breaks of plasmid pUC 9.1 DNA and in vivo by micronucleus test in bone marrow cells of Wistar rats. Cytotoxicity in bone marrows was assessed by scoring polychromatic and normochromatic erythrocytes ratio. An HPLC-DAD method was adapted and validated for the enhancement of coumarin selectivity from the other matrix constituents. The microbial load was satisfactorily reduced, leading to sterilization at the highest dose. Genotoxic and cytotoxic effects were not increased in the in vitro and in vivo models. The concentration of coumarin and the chromatographic profiles of the hydroalcoholic plant extracts (ethanol 70% v/v) were not modified after such process. Therefore, this work suggests that gamma irradiation of M. glomerata raw material is suitable and safe for microbiological control purposes at the employed doses.

scholarly journals Herbal mouthwash based on Libidibia ferrea: microbiological control, sensory characteristics, sedimentation, pH and density

2015 ◽  
Vol 44 (2) ◽  
pp. 118-124 ◽  
Author(s):  
Gisely Naura Venâncio ◽  
Isis Costa Rodrigues ◽  
Tatiane Pereira de Souza ◽  
Raquel de Oliveira Marreiro ◽  
Maria Fulgência Costa Lima Bandeira ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Herbal Medicines ◽  
Mouth Diseases ◽  
Number Of Microorganisms ◽  
Microbiological Control ◽  
Density Values ◽  
Density Evaluation ◽  
Therapeutic Properties ◽  
And Staphylococcus Aureus

Introduction Phytotherapy is the study of herbal medicines and their applicability to cure diseases in general, being a therapeutic method which can be used for the prevention and treatment of mouth diseases. Among the herbal studied, the Libidibia ferrea, known as jucá or ironwood, is widely used in folk medicine by presenting anti-inflammatory, analgesic, antimicrobial and antipyretic therapeutic properties. Objective To evaluate in vitro pharmacological stability of the Libidibia ferrea extract’s mouthwash (INPA - 228 022). Material and method It was held the mouthwash microbiological control by determining the total number of microorganisms and Salmonella sp, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus; stability characteristics (color, odor, brightness and consistency), sedimentation test (centrifuge), the pH measurement (pH meter) and density evaluation (pycnometer) were analyzed. Result The mouthwash showed to be absent from microorganisms and no changes were observed in the organoleptics and sedimentation characteristics. The average pH values were 6.21, 6.15 and 5.85 at 0, 30 and 60 days, respectively, and 1.029, 1.033 and 1.035 g/ mL density values, respectively, without interfering with the final characteristic of the formulation. Conclusion The mouthwash presented pharmacological stability and quality conditions.

Measurement of Alkali Labile DNA Damage and Cross-Links Following 2450 MHz Microwave and Low Dose Gamma Irradiation In Vitro

2006 ◽  
pp. 135-142
Author(s):  
I. Lagroye ◽  
B. Wettring ◽  
E. G. Moros ◽  
W. L. Straube ◽  
W. F. Pickard ◽  
...  
Keyword(s):  
Dna Damage ◽  
Gamma Irradiation ◽  
Low Dose ◽  
Cross Links

scholarly journals The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.)

2020 ◽  
Vol 10 (2) ◽  
pp. 144-149
Author(s):  
Yunia Efrice Banyo Efrice Banyo ◽  
◽  
Serafinah Indriyani ◽  
Wahyu Widoretno ◽  
◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Pogostemon Cablin

Effects of gamma irradiation on dermal equivalents in vitro

1991 ◽  
Vol 47 (7) ◽  
pp. 725-728 ◽  
Author(s):  
T. D. Nguyen ◽  
J. Cornillet-Stoupy ◽  
P. Gillery ◽  
F. X. Maquart
Keyword(s):  
Gamma Irradiation ◽  
Dermal Equivalents

The Effects of Low Doses of Gamma Irradiation on the Ultrastructure ofNosema Apis in Vitro

1990 ◽  
Vol 29 (3) ◽  
pp. 165-171 ◽  
Author(s):  
T. P. Liu ◽  
T. A. Batchelor ◽  
R. J. Munn ◽  
J. M. Marston ◽  
C. L. Judson
Keyword(s):  
Gamma Irradiation ◽  
Low Doses

Immobilized Cratylia mollis lectin: An affinity matrix to purify a soybean (Glycine max) seed protein with in vitro platelet antiaggregation and anticoagulant activities

2011 ◽  
Vol 46 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Mariana C.C. Silva ◽  
Lucimeire A. Santana ◽  
Rosemeire A. Silva-Lucca ◽  
Amanda L.R. Lima ◽  
Joana G. Ferreira ◽  
...  
Keyword(s):  
Glycine Max ◽  
Seed Protein ◽  
Affinity Matrix ◽  
Cratylia Mollis
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