scholarly journals Genetic Variation in <i>Salamandra</i> <i>infraimmaculata</i> from Different Habitats Using Amplified Fragment Length Polymorphism

2014 ◽  
Vol 05 (02) ◽  
pp. 54-66 ◽  
Author(s):  
Gad Degani ◽  
Tali Goldberg ◽  
Eviatar Nevo
Keyword(s):  
Genetic Variation ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism

scholarly journals Genetic diversity of three European Veratrum species revealed by Amplified Fragment Length Polymorphism

2017 ◽  
Vol 47 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Magdalena Szeliga ◽  
Joanna Ciura ◽  
Mirosław Tyrka
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Amplified Fragment Length Polymorphism ◽  
Veratrum Album ◽  
Metabolic Properties

Abstract Chemical and genetic characterization of Veratrum species deposited in European collections is important for genepool preservation and identification of populations with desired metabolic properties. Veratrum album, V. lobelianum and V. nigrum are native to Europe, and in Poland are ranked as rare or threatened. Genetic variation of European Veratrum species was characterized by Amplified Fragment Length Polymorphism (AFLP) markers. The accumulation of jervine as a representative of steroidal alkaloids was measured in seeds. Distribution of 380 markers generated from eight primer combinations was useful for studying genetic relationships among and within species in the Veratrum genus and the most divergent populations were identified. Genetic variation between 12 populations of Veratrum species supports the classification of V. lobelianum as a subspecies of V. album. However, the results need further validation on extended material. A higher genetic diversity (22.3%) was observed between populations of V. nigrum as compared to V. album (14.5%). Contents of jervine allowed for discrimination of the studied Veratrum species and can be used as a potential chemotaxonomic marker. The highest jervine levels were found in V. album. V. nigrum seeds had only trace amounts and no jervine was detected in seeds of V. lobelianum.

scholarly journals Distribution, habitat profile and genetic variability of Namibian succulent Lithops ruschiorum

Bothalia ◽  
2019 ◽  
Vol 49 (1) ◽  
Author(s):  
Sonja Loots ◽  
Christiane M. Ritz ◽  
Michaela Schwager ◽  
Jasna Sehic ◽  
Veit Herklotz ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Length Polymorphism ◽  
Soil Texture ◽  
Fragment Length ◽  
Plant Density ◽  
Fragment Length Polymorphism ◽  
Conservation Status ◽  
Amplified Fragment Length Polymorphism ◽  
Strong Impact ◽  
Field Surveys

Background: The species-rich flora of southern Africa comprises a high number of endemics, including succulents such as the flowering stones in the genus Lithops, but conservation status for these species is not well underpinned because detailed field data and assessments of genetic diversity are lacking.Objectives: We wanted to assess plant abundance and identify factors that may affect survival in Lithops ruschiorum through carefully conducted field surveys, and to determine amount and partitioning of genetic variation by amplified fragment length polymorphism (AFLP) analysis.Method: Field surveys were carried out in nine populations in Namibia. The most meticulously studied population was divided into 51 sites, while another 43 sites were recognised in the remaining eight populations. At each site, occupied area and number of plants were recorded as well as altitude, aspect, slope, soil texture and substrate. Amplified fragment length polymorphism markers were employed to study 52 individuals from seven populations.Results: In total, 8465 individuals were recorded. Plant density and/or plant number was associated with aspect, slope, soil texture, substrate and geographic distance from the coast. Analysis of molecular variation showed that 95% of the variability occurs within populations. Genetic and geographic distances among populations were correlated suggesting an isolation-by-distance pattern.Conclusion: Results are concordant with a strong impact of fog-based precipitation on plant density in the coastal populations, whereas rain probably is more important at one population which is situated further inland. Within-population genetic variation was medium high as usually reported for perennial, outcrossing species, but the low population differentiation implies considerable gene flow and/or population fragmentation.

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