scholarly journals <i>In vivo</i> effects of genistein, herbimycin a and geldanamycin on rat hepatic cytochrome P4501A

2012 ◽  
Vol 03 (04) ◽  
pp. 334-340 ◽  
Author(s):  
Maria L. Perepechaeva ◽  
Alevtina Y. Grishanova
Keyword(s):  
Herbimycin A ◽  
Cytochrome P4501a ◽  
Hepatic Cytochrome

scholarly journals Chemical modification of herbimycin A. Synthesis and in vivo antitumor activities of halogenated and other related derivatives of herbimycin A.

1986 ◽  
Vol 39 (3) ◽  
pp. 415-423 ◽  
Author(s):  
KIYOSHI SHIBATA ◽  
SADAYOSHI SATSUMABAYASHI ◽  
HIROSHI SANO ◽  
KANKI KOMIYAMA ◽  
AKIRA NAKAGAWA ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Antitumor Activities ◽  
Herbimycin A ◽  
Derivatives Of

Relationship between flow rate and NO production in postnatal mesenteric arteries

2001 ◽  
Vol 280 (1) ◽  
pp. G43-G50 ◽  
Author(s):  
Kristina M. Reber ◽  
Gennifer M. Mager ◽  
Charles E. Miller ◽  
Philip T. Nowicki
Keyword(s):  
Flow Rate ◽  
Kinase Inhibitor ◽  
Age Groups ◽  
Mesenteric Arteries ◽  
No Production ◽  
Herbimycin A ◽  
Wide Range ◽  
Intracellular Ca ◽  
Controlled Flow

We studied mesenteric arterial arcades from 3- and 35-day-old swine to determine the relationship between perfusate flow rate and release of nitric oxide (NO) into mesenteric effluent. Mesenteric arterial arcades were perfused under controlled-flow conditions with a peristaltic pump using warm oxygenated Krebs buffer. Basal rates of NO production were 43.6 ± 4.2 vs. 12.1 ± 2.5 nmol/min in 3- vs. 35-day-old mesentery during perfusion at in vivo flow rates (9 vs. 20 ml/min, respectively). Rate of NO production was directly related to flow rate over a wide range of flows (5–40 ml/min) in 3- but not 35-day-old mesentery. Both age groups demonstrated a brisk, albeit brief, increase in NO production in response to infusion of NO-dependent vasodilator substance P (10−8 M/min). Tyrosine kinase inhibitor herbimycin A andl-arginine analog l-NMMA significantly attenuated flow-induced increase in NO production, and phosphatase inhibitor phenylarsine oxide increased magnitude of flow-induced increase in NO production in 3-day-olds. Removal of extracellular Ca2+ and depletion of intracellular Ca2+ stores (Ca2+-free Krebs with EGTA plus thapsigargin) had no effect on NO production in either group. Thus, basal rate of NO production is greater in mesenteric arterial arcades from 3- than from 35-day old swine, a direct relationship between flow rate and NO production rate is present in mesentery from 3- but not 35-day-olds, and phosphorylation events are necessary for this interaction to occur.

In Vivo Interactions of Aluminum with Hepatic Cytochrome P-450 and Metallothionein

1987 ◽  
Vol 8 (4) ◽  
pp. 541-548
Author(s):  
E. H. JEFFERY ◽  
H. T. JANSEN ◽  
J. A. DELLINGER
Keyword(s):  
Cytochrome P 450 ◽  
Hepatic Cytochrome

scholarly journals beta-Naphthoflavone abolishes interrenal sensitivity to ACTH stimulation in rainbow trout

1998 ◽  
Vol 157 (1) ◽  
pp. 63-70 ◽  
Author(s):  
JM Wilson ◽  
MM Vijayan ◽  
CJ Kennedy ◽  
GK Iwama ◽  
TW Moon
Keyword(s):  
Cytochrome P450 ◽  
Rainbow Trout ◽  
Acth Stimulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Treated Fish ◽  
First Time ◽  
Hepatic Cytochrome ◽  
Stimulation Of

We report for the first time that beta-naphthoflavone (BNF) abolishes ACTH stimulation of cortisol production in rainbow trout (Oncorhynchus mykiss). There was significantly higher hepatic cytochrome P450 content and ethoxyresorufin O-de-ethylase and uridine-5'-diphosphoglucuronic acid transferase activities in BNF-treated fish than in sham-treated controls. BNF did not significantly affect either plasma turnover or tissue distribution of [3H]cortisol-derived radioactivity. Hepatic membrane fluidity and hepatocyte capacity for cortisol uptake were not altered by BNF as compared with the sham-treated fish. These results taken together suggest that BNF does not affect cortisol-clearance mechanisms in trout. A 3 min handling disturbance period elicited a plasma cortisol response in the sham-treated fish; however, the response in the BNF-treated fish was muted and significantly lower than in the sham fish. This in vivo response corroborates the lack of interrenal sensitivity to ACTH in vitro in the BNF-treated fish, suggesting that BNF affects the ACTH pathway in trout. Our results suggest the possibility that cytochrome P450-inducing compounds may affect cortisol dynamics by decreasing interrenal responsiveness to ACTH stimulation in fish, thereby impairing the physiological responses that are necessary for the animal to cope with the stressor.

Sign in / Sign up

Export Citation Format

Share Document