scholarly journals P53 protein expression and cell viability in irradiated peripheral blood mononuclear cells as bioindicators of radiosensitivity

2011 ◽  
Vol 02 (02) ◽  
pp. 63-67 ◽  
Author(s):  
Mariana Brayner Cavalcanti ◽  
Ana Paula Galvão da Silva ◽  
Rafael de Freitas e Silva ◽  
Ademir Amaral
Keyword(s):  
Protein Expression ◽  
Cell Viability ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
P53 Protein ◽  
Peripheral Blood Mononuclear ◽  
P53 Protein Expression ◽  
Blood Mononuclear Cells

Analysis of Protein Expression in Human Cells Cocultured with Porcine Peripheral Blood Mononuclear Cells

Intervirology ◽  
2018 ◽  
Vol 61 (5) ◽  
pp. 237-246
Author(s):  
Yuyuan Ma ◽  
Xiong Zhao ◽  
Junting Jia ◽  
Yongxian Yang ◽  
Rui Fan ◽  
...  
Keyword(s):  
Protein Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Kaempferol derivatives isolated from Lens culinaris Medik. reduce DNA damage induced by etoposide in peripheral blood mononuclear cells

2019 ◽  
Vol 8 (6) ◽  
pp. 896-907 ◽  
Author(s):  
Magdalena Kluska ◽  
Michał Juszczak ◽  
Daniel Wysokiński ◽  
Jerzy Żuchowski ◽  
Anna Stochmal ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Viability ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Lens Culinaris ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Aerial Parts ◽  
Kaempferol Glycoside ◽  
Blood Mononuclear Cells

Abstract Bioactive compounds isolated from plants are considered to be attractive candidates for cancer therapy. In this study, we examined the effect of kaempferol, its derivatives, the polyphenol fraction (PF) and an extract (EX) isolated from the aerial parts of Lens culinaris Medik. on DNA damage induced by etoposide in human cells. We also studied the effect of these compounds and their combinations on cell viability. The studies were conducted on HL-60 cells and human peripheral blood mononuclear cells (PBMCs). We used the comet assay in the alkaline version to evaluate DNA damage. To examine cell viability we applied the trypan blue exclusion assay. We demonstrated that kaempferol glycoside derivatives isolated from the aerial parts of Lens culinaris Medik. reduce DNA damage induced by etoposide in PBMCs, but do not have an impact on DNA damage in HL-60 cells. We also showed that kaempferol induces DNA damage in HL-60 cells and leads to an increase of DNA damage provoked by etoposide. Our data suggest that kaempferol derivatives can be further explored as a potential agent protecting normal cells against DNA damage induced by etoposide. Moreover, kaempferol's ability to induce DNA damage in cancer cells and to increase DNA damage caused by etoposide may be useful in designing and improving anticancer therapies.

Interaction of hypothalamic Na,K-ATPase inhibitor with isolated human peripheral blood mononuclear cells

1994 ◽  
Vol 14 (5) ◽  
pp. 231-242 ◽  
Author(s):  
Beatrice M. Anner ◽  
Danielle Lacotte ◽  
Rolf M. Anner ◽  
Marlis Moosmayer
Keyword(s):  
Cell Viability ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Exchange Process ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Isolated Human Lymphocytes

A ligand for the digitalis receptor located on the membrane-embedded Na,K-ATPase (NKA; EC 3.6.1.37) has been isolated from bovine hypothalamus (hypothalamic inhibitory factor; HIF) and identified as isomeric ouabain (Tymiaket al, 1993,Proc. Natl. Acad. Sci.90: 8189–8193). In analogy to cardioactive steroids (CS) derived from plants or from toad, HIF inhibits the Na/K-exchange process and the ATPase activity of isolated Na,K-ATPase although by a different molecular action mechanism. In the present work we show that, as plant-derived ouabain, HIF inhibits86Rb-uptake by isolated human lymphocytes with an IC50 of about 20 nM; above this concentration HIF reduces cell viability in contrast to ouabain. The decrease in cell viability by excess HIF is accompanied by discrete morphological alterations (mitochondrial swelling) visible by transmission electron microscopy of ultra-thin sectioned peripheral blood mononuclear cells. Taken together the results show that the hypothalamic NKA inhibitor blocks NKA of isolated human lymphocytes with high potency at nanomolar concentrations without toxicity; concentrations exceeding the ones required to block86Rb-uptake reduce cell viability, probably due to leak formation across the NKA molecule. Thus, lymphocytes constitute a potential target for HIF action and by their altered NKA status a possible messenger between the nervous and the immune system.

scholarly journals The effect of aging on the autophagic and heat shock response in human peripheral blood mononuclear cells

2018 ◽  
Vol 105 (3) ◽  
pp. 247-256 ◽  
Author(s):  
JJ McCormick ◽  
TA VanDusseldorp ◽  
CG Ulrich ◽  
RL Lanphere ◽  
K Dokladny ◽  
...  
Keyword(s):  
Heat Shock ◽  
Protein Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Heat Shock Response ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Mrna Levels ◽  
Peripheral Blood Mononuclear ◽  
Shock Response ◽  
Blood Mononuclear Cells

Autophagy is a lysosome degradation pathway through which damaged organelles and macromolecules are degraded within the cell. A decrease in activity of the autophagic process has been linked to several age-associated pathologies, including triglyceride accumulation, mitochondrial dysfunction, muscle degeneration, and cardiac malfunction. Here, we examined the differences in the autophagic response using autophagy-inducer rapamycin (Rapa) in peripheral blood mononuclear cells (PBMCs) from young (21.8 ± 1.9 years) and old (64.0 ± 3.7 years) individuals. Furthermore, we tested the interplay between the heat shock response and autophagy systems. Our results showed a significant increase in LC3-II protein expression in response to Rapa treatment in young but not in old individuals. This was associated with a decreased response in MAP1LC3B mRNA levels, but not SQSTM1/p62. Furthermore, HSPA1A mRNA was upregulated only in young individuals, despite no differences in HSP70 protein expression. The combined findings suggest a suppressed autophagic response following Rapa treatment in older individuals.

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