scholarly journals Decrease in the Hospital Stay of Neonates with Suspected Nosocomial Sepsis with the Use of a Molecular Biology Technique

2019 ◽  
Vol 07 (03) ◽  
pp. 44-51
Author(s):  
Jesús Reyna-Figueroa ◽  
María Fernanda Rodríguez-Sánchez ◽  
Pilar Miyoko Martínez Matsumoto ◽  
Federico Javier Ortiz-Ibarra ◽  
Ana Elena Limón-Rojas
Keyword(s):  
Molecular Biology ◽  
Hospital Stay ◽  
Molecular Biology Technique ◽  
Nosocomial Sepsis

scholarly journals Varietal Identification of Cyclamen and Petunia Seeds Using RAPDs

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 574d-574
Author(s):  
Jianhua Zhang ◽  
Miller B. McDonald
Keyword(s):  
Molecular Biology ◽  
Genetic Variability ◽  
Seed Production ◽  
Seed Quality ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Purity ◽  
Molecular Biology Technique ◽  
Banding Patterns ◽  
Varietal Identification

Varietal identification of cyclamen and petunia is important for flower seed production because these crops are marketed as hybrids and genetic purity determinations assure the purity of the seed lot and the success of hybridization. Random amplified polymorphic DNA (RAPD) banding patterns have been shown to be useful in identifying genotypes of various crops. This molecular biology technique was applied to five commercial cyclamen and six petunia hybrids. Using bulk seed/seedling samples, the varieties could be differentiated. However, when individual seeds of the cyclamen hybrid were tested, differing polymorphisms were observed. These variations were attributed to genetic variability in the inbred parents. We conclude that the genetic purity of cyclamen seeds can be improved and that the use of RAPDs can assist breeders of hybrid flowering crops in better monitoring seed quality.

Development and Application of Molecular Biology Techniques in Environmental Treatment

2014 ◽  
Vol 587-589 ◽  
pp. 892-895
Author(s):  
Yang Yu ◽  
Hai Jiao Yu
Keyword(s):  
Molecular Biology ◽  
Pollution Control ◽  
Deep Understanding ◽  
Point Of View ◽  
Molecular Biology Technique ◽  
Control Practice ◽  
Environmental Treatment ◽  
Biochemical Mechanisms ◽  
Physiological And Biochemical Mechanisms ◽  
Physiological And Biochemical

The application of molecular biology technique to expand the breadth and depth of the study of environmental microbiology, the researchers from the microscopic point of view more detailed deep understanding of the physiological and biochemical mechanisms of microorganisms on the degradation of pollutants, and at the same time, the advanced molecular biology technology for environmental monitoring, environmental pollution treatment, bioremediation of the contaminated environment provides the basis and method for faster, more sensitive, more scientific, which greatly promoted the progress of pollution control practice. The basic theory of molecular biology technology and its application in wastewater through the detection of microorganisms are shown.

scholarly journals How the techniques of molecular biology are developed from natural systems

2018 ◽  
Author(s):  
Isobel Ronai
Keyword(s):  
Nucleic Acid ◽  
Molecular Biology ◽  
Molecular Mechanisms ◽  
Fluorescent Proteins ◽  
Restriction Enzymes ◽  
Developmental Trajectory ◽  
Molecular Biology Technique ◽  
Natural Systems ◽  
Biological Specificity ◽  
Specificity Protein

A striking characteristic of the highly successful techniques in molecular biology is that they are derived from natural occurring systems. RNA interference (RNAi), for example, utilises a mechanism that evolved in eukaryotes to destroy foreign nucleic acid. Other examples include restriction enzymes, the polymerase chain reaction, fluorescent proteins and CRISPR-Cas9. I propose that natural molecular mechanisms are exploited by biologists for their effectors’ (protein or nucleic acid) activity and biological specificity (protein or nucleic acid can cause precise reactions). I also show that the developmental trajectory of novel techniques in molecular biology, such as RNAi, is four characteristic phases. The first phase is discovery of a biological phenomenon. The second is identification of the mechanism’s trigger(s), the effector and biological specificity. The third is the application of the technique. The final phase is the maturation and refinement of the molecular biology technique. The development of new molecular biology techniques from nature is crucial for both biological and biomedical research.

scholarly journals Veterinary molecular laboratory examination

2018 ◽  
pp. 087-097
Author(s):  
Tagesu Abdisa
Keyword(s):  
Molecular Biology ◽  
Disease Process ◽  
Laboratory Examination ◽  
Molecular Biology Technique ◽  
Trained Personnel ◽  
Causative Agents ◽  
Molecular Biological Techniques

Molecular biological techniques are the best dimensions to diagnosis the disease process and its causative agents. The molecular biology technique is performed with sophisticated instruments or equipment’s required specialized trained personnel and undertaken in routine diagnosistic laboratories.

scholarly journals Biodiversity and Dynamics (Rate of Change) of Bacterial Communities Involved in the Biodegradation of Petroleum Refinery Sludge in Contaminated Soils

2020 ◽  
pp. 23-38
Author(s):  
Tudararo-Aherobo Laurelta ◽  
Atuayan Ernest ◽  
Adetutu Eric ◽  
Ball Andrew
Keyword(s):  
Molecular Biology ◽  
Contaminated Soils ◽  
Bacterial Communities ◽  
Petroleum Refinery ◽  
Rate Of Change ◽  
Pseudomonas Sp ◽  
Molecular Biology Technique ◽  
Moving Window Analysis ◽  
Significant Difference ◽  
Culture Independent

Aim: The study assessed the bio treatability of the petroleum refinery sludge in contaminated soils by indigenous bacterial communities and the effects of the sludge contamination and bio stimulants on the biodiversity and dynamics (rate of change) of the bacterial communities involved in the biodegradation of the sludge, using the molecular biology technique, Denaturing Gradient Gel Electrophoresis (DGGE). Study Design: The randomnized block design was used for the study. Place and duration of the Study: The research was conducted in the biology laboratory of Flinders University, Adelaide, South Australia. Methodology: The percentage of total petroleum hydrocarbons (TPH) degraded and the bacterial load in the test microcosms was assessed tri-weekly for 12 weeks. The percentage TPH was assessed using Gas chromatography, while the bacterial count was determined as gene copies using the culture independent molecular tool, quantitative real-time PCR (qRt-PCR) analysis. The effects of the experimental treatments on the biodiversity and dynamics (rate of change) of the bacterial communities involved in the biodegradation of the sludge in the soils was determined by the culture-independent molecular biology technique, DGGE. Moving Windows Analysis (MWA) and Shannon Weaver diversity index were used to determine the dynamics (rate of change) and biodiversity of the bacterial communities respectively. Results: Results obtained for the Moving Window Analysis (MWA) which is used to determine the dynamics (Dy), or rate of change of the bacterial communities, showed that, the 1% and 5% sludge contaminated soils biostimulated with compost, recorded the highest Dy of 86.0 ± 1.90% and 87.0 ± 2.20% respectively.NPK biostimilated soil microcosms however recorded a lower Dy of 33.75± 3.20 and 32.50 ± 4.68% for 1% and 5% sludge contamination respectively. The biodiversity of the bacterial communities expressed as Shannon -Weaver index (H1), recorded the highest value of 2.76 ±0.02 for the compost biostimulated microcosm in the 1% sludge treatment, while for the 5% sludge contamination, the treatment with NPK and surfactant enhanced the bacterial biodiversity most with a value of 2.76 ±0.07%. In the test soils with 1% sludge contamination, bio stimulation with NPK gave the highest % TPH degradation (78.25%) while the treatment with NPK and Triton-X 100 had the highest TPH degradation (46.55%) for the 5% sludge contaminated soils. There was insignificant difference in the % sludge degradation between the control and other treatments at P > 0.05 and F = 4.07 for the 1% sludge treated soils, while for the soils treated with 5% sludge there was significant difference between the control and other treatments at P < 0.05 and F= 4.07. Conclusion: Bacteria species identified in the sludge by molecular biology techniques included; Pseudomonas sp. ITRI77, Uncultured Thauera sp., Uncultured Pseudomonas sp., Flavobacterium sp., Bacillaceae bacterium, Uncultured soil bacterium, Clostridium sp., most of which are Gram negative. Biostimulation with compost enhanced a higher biodiversity (H i) and dynamics (Dy) of the bacterial communities involved in the biodegradation of the sludge. Though the NPK treated soils enhanced the biodegradation of the sludge most, degradation started declining by the 9th week while that of compost continued to rise steadily till the 12th week. Results obtained indicate that compost is as good as NPK in the biodegradation of petroleum sludge especially at 1% sludge contamination, since there was no statistical difference between the % TPH degraded and the use of compost is environmentally friendly and economically sustainable.

scholarly journals NLRP3, IL-1β, and Caspase-1 Gene Transcript Identification and Expression by QCM-D in Obese Children

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Modesto Gómez López ◽  
Nadia Mabel Pérez-Vielma ◽  
Haydee González Martínez ◽  
Eleazar Lara Padilla ◽  
Cindy Bandala ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Biology ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Gene Expression Pattern ◽  
Gene Expression Patterns ◽  
Gene Transcript ◽  
Molecular Biology Technique ◽  
Caspase 1 ◽  
Gene And Protein Expression

Background. Obesity in children is highly prevalent in Mexican population. Adipose tissue has been related to specific pro- and anti-inflammatory cytokine and inflammasome gene and protein expression patterns. Actually, there is no existing biosensor for detecting gene expression patterns in children with obesity. The quartz crystal microbalance with dissipation monitoring (QCM-D) has been used as a transducer for DNA biosensor design. Results. In this study, the gene expression pattern of IL-1β, NLRP3, and CASPASE-1 in children with obesity was successfully determined by means of QCM-D. Gene expression patterns were validated with those obtained by quantitative polymerase chain reaction (qPCR), a validated molecular biology technique for gene expression quantification. QCM-D analysis of the detected mass corresponding results for each of the genes showed a major detected mass for IL-1β, followed by similar NLRP3 and constitutive gene 18S deposited mass and a smaller deposited mass for CASPASE-1. Surprisingly, when comparing mRNA gene expression results for NLRP3, IL-1β, and CASPASE-1 obtained with qPCR and QCM-D, similar patterns were found, revealing greatest expression of IL-1β, followed by NLRP3, with CASPASE-1 being the molecule of least expression in the group of children with obesity. AFM images illustrate the step-by-step changes that took place on the quartz surface. Conclusions. QCM-D proved successfully for determining the gene transcripts and expression of NLRP3, IL-1β, and CASPASE-1 in children with obesity, with similar results validated by qPCR. “QCM-D decreases detection costs compared with a validated molecular biology technique.” The QCM-D biosensor developed by our group was successful for gene expression determination; in the future, it can be used for molecular diagnosis.

scholarly journals Isolation, identification and investigation some biochemical properties of lactic acid bacteria from “Mam sac chua” and “Com me” in Tien Giang province

2021 ◽  
Vol 11 (1) ◽  
pp. 11-20
Author(s):  
Truong Quoc Tat ◽  
Nguyen Duy Khanh
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Molecular Biology ◽  
Lactobacillus Plantarum ◽  
Salinity Tolerance ◽  
Salt Concentration ◽  
Biochemical Properties ◽  
Gram Positive ◽  
Molecular Biology Technique ◽  
Mrs Broth

The objective of this study was isolate, identify and investigate some biochemical properties of strains of lactic acid bacteria from “com me” and “mam sac chua” in Tien Giang province. Nineteen strains of LAB were isolated from four “com me” and three “mam sac chua” samples. They have characterized of lactic acid bacteria such as: halo rings in MRS agar environment added 0,85% CaCO3, rod-shaped cells, Gram positive, catalate and oxidase negative. All 19 strains of LAB were able to produce lactic acid in MRS broth (1,01 – 2,23 mg/ mL after 24 hours). Three strains of LAB were isolated from “com me” were able to produce lactic acid in MRS broth at salt concentration of 0, 2, 4 and 6% (0,57 – 1,29 mg/ mL after 24 hours). In particular, strains of LAB were coded ML3 and ML4 produced the highest lactic acid and VB strain was the most salinity tolerance. Therefore, these three srains were choosed to identify species by molecular biology technique. The results of identification were Staphylococcus piscifermentans VB, Lactobacillus plantarum ML3 and Lactobacillus plantarum ML4 because they are 99% homologous to S. piscifermentans and L. plantarum.

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