scholarly journals Physiological Effects of Salmon Milt Nucleoprotein on Movement, Stress Tolerance and Lifespan of <i>C. elegans</i>

2012 ◽  
Vol 03 (01) ◽  
pp. 48-54 ◽  
Author(s):  
Hiroaki Shintani ◽  
Tsubasa Furuhashi ◽  
Hiroyuki Hano ◽  
Masaji Matsunaga ◽  
Koji Usumi ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Physiological Effects ◽  
C Elegans

scholarly journals Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16

2018 ◽  
Vol 120 (8) ◽  
pp. 872-880 ◽  
Author(s):  
Takaya Sugawara ◽  
Kazuichi Sakamoto
Keyword(s):  
Caenorhabditis Elegans ◽  
Life Span ◽  
Stress Tolerance ◽  
Bifidobacterium Longum ◽  
Intestinal Flora ◽  
Dependent Manner ◽  
Physiological Effects ◽  
E Coli ◽  
C Elegans ◽  
Reduced Body

AbstractProbiotics are bacteria among the intestinal flora that are beneficial for human health. Bifidobacterium longum (BL) is a prototypical probiotic that is widely used in yogurt making, supplements and others. Although various physiological effects of BL have been reported, those associated with longevity and anti-ageing still remain elusive. Here we aimed to elucidate the physiological effects of killed BL (BR-108) on stress tolerance and longevity of Caenorhabditis elegans and their mechanisms. Worms fed killed BL in addition to Escherichia coli (OP50) displayed reduced body length in a BL dose-dependent manner. When compared with those fed E. coli alone, these worms had a higher survival rate following heat stress at 35°C and hydrogen peroxide-induced oxidative stress. A general decrease in motility was observed over time in all worms; however, killed BL-fed ageing worms displayed increased movement and longer life span than those fed E. coli alone. However, the longevity effect was suppressed in sir-2.1, daf-16 and skn-1-deficient worms. Killed BL induced DAF-16 nuclear localisation and increased the expression of the DAF-16 target gene hsp-12.6. These results revealed that the physiological effects of killed BL in C. elegans were mediated by DAF-16 activation. These findings contradict previous observations with different Bifidobacterium and Lactobacillus strains, which showed the role for SKN-1 independently of DAF-16.

scholarly journals Inhibitory peptidergic modulation of C. elegans serotonin neurons is gated by T-type calcium channels

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Kara E Zang ◽  
Elver Ho ◽  
Niels Ringstad
Keyword(s):  
Calcium Channels ◽  
Reproductive Behavior ◽  
Serotonin Release ◽  
Physiological Effects ◽  
Cellular Targets ◽  
C Elegans ◽  
Inhibitory Modulation ◽  
Serotonin Neurons ◽  
Behavioral States

Serotonin is an evolutionarily ancient molecule that functions in generating and modulating many behavioral states. Although much is known about how serotonin acts on its cellular targets, how serotonin release is regulated in vivo remains poorly understood. In the nematode C. elegans, serotonin neurons that drive female reproductive behavior are directly modulated by inhibitory neuropeptides. Here, we report the isolation of mutants in which inhibitory neuropeptides fail to properly modulate serotonin neurons and the behavior they mediate. The corresponding mutations affect the T-type calcium channel CCA-1 and symmetrically re-tune its voltage-dependencies of activation and inactivation towards more hyperpolarized potentials. This shift in voltage dependency strongly and specifically bypasses the behavioral and cell physiological effects of peptidergic inhibition on serotonin neurons. Our results indicate that T-type calcium channels are critical regulators of a C. elegans serotonergic circuit and demonstrate a mechanism in which T-type channels functionally gate inhibitory modulation in vivo.

scholarly journals Extreme Depletion of PIP3 Accompanies the Increased Life Span and Stress Tolerance of PI3K-null C. elegans Mutants

2013 ◽  
Vol 4 ◽  
Author(s):  
Puneet Bharill ◽  
Srinivas Ayyadevara ◽  
Ramani Alla ◽  
Robert J. Shmookler Reis
Keyword(s):  
Life Span ◽  
Stress Tolerance ◽  
C Elegans

Reserpine can confer stress tolerance and lifespan extension in the nematode C. elegans

2008 ◽  
Vol 9 (5) ◽  
pp. 309-316 ◽  
Author(s):  
Deepti Srivastava ◽  
Upasna Arya ◽  
Thangavelu SoundaraRajan ◽  
Hemalata Dwivedi ◽  
Sandeep Kumar ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Lifespan Extension ◽  
C Elegans

Corrigendum to ‘Effects of chlorogenic acid on thermal stress tolerance in C. elegans via HIF-1, HSF-1 and autophagy’ [Phytomedicine 66 (2020) 153132]

Phytomedicine ◽  
2020 ◽  
Vol 69 ◽  
pp. 153204
Author(s):  
Andrea del Valle Carranza ◽  
Alejandra Saragusti ◽  
Gustavo Alberto Chiabrando ◽  
Fernando Carrari ◽  
Ramón Asis
Keyword(s):  
Thermal Stress ◽  
Chlorogenic Acid ◽  
Stress Tolerance ◽  
C Elegans

scholarly journals Ginseng Extract Ameliorates the Negative Physiological Effects of Heat Stress by Supporting Heat Shock Response and Improving Intestinal Barrier Integrity: Evidence from Studies with Heat-Stressed Caco-2 Cells, C. elegans and Growing Broilers

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 835 ◽  
Author(s):  
Georg Sandner ◽  
Andreas S. Mueller ◽  
Xiaodan Zhou ◽  
Verena Stadlbauer ◽  
Bettina Schwarzinger ◽  
...  
Keyword(s):  
Heat Stress ◽  
Caenorhabditis Elegans ◽  
Heat Shock ◽  
Tight Junction ◽  
Intestinal Barrier ◽  
Physiological Effects ◽  
Tight Junction Proteins ◽  
Ginseng Extract ◽  
C Elegans ◽  
Junction Proteins

Climatic changes and heat stress have become a great challenge in the livestock industry, negatively affecting, in particular, poultry feed intake and intestinal barrier malfunction. Recently, phytogenic feed additives were applied to reduce heat stress effects on animal farming. Here, we investigated the effects of ginseng extract using various in vitro and in vivo experiments. Quantitative real-time PCR, transepithelial electrical resistance measurements and survival assays under heat stress conditions were carried out in various model systems, including Caco-2 cells, Caenorhabditis elegans and jejunum samples of broilers. Under heat stress conditions, ginseng treatment lowered the expression of HSPA1A (Caco-2) and the heat shock protein genes hsp-1 and hsp-16.2 (both in C. elegans), while all three of the tested genes encoding tight junction proteins, CLDN3, OCLN and CLDN1 (Caco-2), were upregulated. In addition, we observed prolonged survival under heat stress in Caenorhabditis elegans, and a better performance of growing ginseng-fed broilers by the increased gene expression of selected heat shock and tight junction proteins. The presence of ginseng extract resulted in a reduced decrease in transepithelial resistance under heat shock conditions. Finally, LC-MS analysis was performed to quantitate the most prominent ginsenosides in the extract used for this study, being Re, Rg1, Rc, Rb2 and Rd. In conclusion, ginseng extract was found to be a suitable feed additive in animal nutrition to reduce the negative physiological effects caused by heat stress.

Effects of chlorogenic acid on thermal stress tolerance in C. elegans via HIF-1, HSF-1 and autophagy

Phytomedicine ◽  
2020 ◽  
Vol 66 ◽  
pp. 153132 ◽  
Author(s):  
Andrea del Valle Carranza ◽  
Alejandra Saragusti ◽  
Gustavo Alberto Chiabrando ◽  
Fernando Carrari ◽  
Ramón Asis
Keyword(s):  
Thermal Stress ◽  
Chlorogenic Acid ◽  
Stress Tolerance ◽  
C Elegans

scholarly journals Catalpol Modulates Lifespan via DAF-16/FOXO and SKN-1/Nrf2 Activation inCaenorhabditis elegans

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Hyun Won Seo ◽  
Se Myung Cheon ◽  
Myon-Hee Lee ◽  
Hong Jun Kim ◽  
Hoon Jeon ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Antioxidant Enzyme Activities ◽  
Related Factors ◽  
Pumping Rate ◽  
Resistance Proteins ◽  
C Elegans ◽  
Normal Culture Condition ◽  
Nrf2 Activation ◽  
Pharyngeal Pumping ◽  
Effective Component

Catalpol is an effective component of rehmannia root and known to possess various pharmacological properties. The present study was aimed at investigating the potential effects of catalpol on the lifespan and stress tolerance usingC. elegansmodel system. Herein, catalpol showed potent lifespan extension of wild-type nematode under normal culture condition. In addition, survival rate of catalpol-fed nematodes was significantly elevated compared to untreated control under heat and oxidative stress but not under hyperosmolality conditions. We also found that elevated antioxidant enzyme activities and expressions of stress resistance proteins were attributed to catalpol-mediated increased stress tolerance of nematode. We further investigated whether catalpol’s longevity effect is related to aging-related factors including reproduction, food intake, and growth. Interestingly, catalpol exposure could attenuate pharyngeal pumping rate, indicating that catalpol may induce dietary restriction of nematode. Moreover, locomotory ability of aged nematode was significantly improved by catalpol treatment, while lipofuscin levels were attenuated, suggesting that catalpol may affect age-associated changes of nematode. Our mechanistic studies revealed thatmek-1, daf-2, age-1, daf-16, andskn-1are involved in catalpol-mediated longevity. These results indicate that catalpol extends lifespan and increases stress tolerance ofC. elegansvia DAF-16/FOXO and SKN-1/Nrf activation dependent on insulin/IGF signaling and JNK signaling.

Sign in / Sign up

Export Citation Format

Share Document