scholarly journals Non-Invasive Second Harmonic Generation (SHG) in Macroscopy (MacroSHG) as Bio-Diagnosis to Image Collagen Network Organization in Extracellular Matrix

Engineering ◽  
2014 ◽  
Vol 06 (08) ◽  
pp. 485-490 ◽  
Author(s):  
Dominique Dumas ◽  
Elisabeth Werkmeister ◽  
Sebastien Hupont ◽  
Céline Huselstein ◽  
Natalia De Isla ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Network Organization ◽  
Collagen Network ◽  
Non Invasive

scholarly journals Characterization of Collagen I Fiber Thickness, Density, and Orientation in the Human Skin In Vivo Using Second-Harmonic Generation Imaging

Photonics ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 404
Author(s):  
Marius Kröger ◽  
Johannes Schleusener ◽  
Sora Jung ◽  
Maxim E. Darvin
Keyword(s):  
Extracellular Matrix ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Skin Diseases ◽  
Second Harmonic ◽  
Skin Aging ◽  
Collagen I ◽  
Fast Fourier Transformation ◽  
Occurrence Matrix

The assessment of dermal alterations is necessary to monitor skin aging, cancer, and other skin diseases and alterations. The gold standard of morphologic diagnostics is still histopathology. Here, we proposed parameters to distinguish morphologically different collagen I structures in the extracellular matrix and to characterize varying collagen I structures in the skin with similar SAAID (SHG-to-AF Aging Index of Dermis, SHG—second-harmonic generation; AF—autofluorescence) values. Test datasets for the papillary and reticular extracellular matrix from images in 24 female subjects, 36 to 50 years of age, were generated. Parameters for SAAID, edge detection, and fast Fourier transformation directionality were determined. Additionally, textural analyses based on the grey level co-occurrence matrix (GLCM) were conducted. At first, changes in the GLCM parameters were determined in the native greyscale images and, furthermore, in the Hilbert-transformed images. Our results demonstrate a robust set of parameters for noninvasive in vivo classification for morphologically different collagen I structures in the skin, with similar and different SAAID values. We anticipate our method to enable an automated prevention and monitoring system with an age- and gender-specific algorithm.

scholarly journals Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques

2019 ◽  
Vol 2 (2) ◽  
pp. 49 ◽  
Author(s):  
Mehdi Alizadeh ◽  
Masood Ghotbi ◽  
Pablo Loza-Alvarez ◽  
David Merino
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Imaging Techniques ◽  
Image Plane ◽  
Excitation Beam ◽  
Non Invasive ◽  
Second Harmonic Generation Imaging ◽  
First Time

Polarization sensitive second harmonic generation (pSHG) microscopy is an imaging technique able to provide, in a non-invasive manner, information related to the molecular structure of second harmonic generation (SHG) active structures, many of which are commonly found in biological tissue. The process of acquiring this information by means of pSHG microscopy requires a scan of the sample using different polarizations of the excitation beam. This process can take considerable time in comparison with the dynamics of in vivo processes. Fortunately, single scan polarization sensitive second harmonic generation (SS-pSHG) microscopy has also been reported, and is able to generate the same information at a faster speed compared to pSHG. In this paper, the orientation of second harmonic active supramolecular assemblies in starch granules is obtained on by means of pSHG and SS-pSHG. These results are compared in the forward and backward directions, showing a good agreement in both techniques. This paper shows for the first time, to the best of the authors’ knowledge, data acquired using both techniques over the exact same sample and image plane, so that they can be compared pixel-to-pixel.

scholarly journals Depth resolved label-free multimodal optical imaging platform to study morpho-molecular composition of tissue

2019 ◽  
Vol 18 (5) ◽  
pp. 997-1008 ◽  
Author(s):  
Marco Andreana ◽  
Ryan Sentosa ◽  
Mikael T. Erkkilä ◽  
Wolfgang Drexler ◽  
Angelika Unterhuber
Keyword(s):  
Optical Coherence Tomography ◽  
Raman Scattering ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Structural Information ◽  
Second Harmonic ◽  
Label Free ◽  
Two Photon ◽  
Non Invasive ◽  
Anti Stokes

The presented multi-modal platform combines optical coherence tomography, two-photon excited fluorescence, second harmonic generation and anti-Stokes Raman scattering to provide molecular and structural information of tissue in a fast and non-invasive manner.

Epithelial-derived TGF-β2 modulates basal and wound-healing subepithelial matrix homeostasis

2006 ◽  
Vol 291 (6) ◽  
pp. L1277-L1285 ◽  
Author(s):  
H. Garrett R. Thompson ◽  
Justin D. Mih ◽  
Tatiana B. Krasieva ◽  
Bruce J. Tromberg ◽  
Steven C. George
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Transforming Growth Factor ◽  
Second Harmonic ◽  
Smooth Muscle Actin ◽  
Critical Role ◽  
The Matrix

The epithelium influences the mesenchyme during dynamic processes such as embryogenesis, wound healing, fibrosis, and carcinogenesis. Since transforming growth factor-β (TGF-β) modulates these processes, we hypothesized that epithelial-derived TGF-β also plays a critical role in maintaining the extracellular matrix at basal conditions. We utilized an in vitro model of the epithelial-mesenchymal trophic unit in the human airways to determine the role of epithelial-derived TGF-β in modulating the extracellular matrix under basal and wound-healing conditions. When differentiated at an air-liquid interface, the human bronchial epithelium produces active TGF-β2 at a concentration of 50–70 pg/ml, whereas TGF-β1 is undetectable. TGF-β2 increases two- to threefold following scrape injury in a dose-dependent fashion and significantly enhances both α-smooth muscle actin expression in the underlying collagen-embedded fibroblasts and secretion of tenascin-C into the matrix. Multiphoton microscopy demonstrates substantially enhanced second harmonic generation from fibrillar collagen in the matrix. Pretreatment of the matrix with either sirolimus (2.5 nM) or paclitaxel (10 nM) abolishes the increases in both TGF-β2 and second harmonic generation in response to epithelial injury. In the absence of the epithelium, exogenous active TGF-β2 (0–400 pg/ml) produces a biphasic response in the second harmonic signal with a minimum occurring at the epithelial-derived basal level. We conclude that epithelial-derived TGF-β2 is secreted in response to injury, significantly alters the bulk optical properties of the extracellular matrix, and its tight regulation may be required for normal collagen homeostasis.

Tumor extracellular matrix: lessons from the second-harmonic generation microscopy

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Rodrigo de Andrade Natal ◽  
Javier Adur ◽  
Carlos Lenz Cesar ◽  
José Vassallo
Keyword(s):  
Extracellular Matrix ◽  
Second Harmonic Generation ◽  
Cancer Cell ◽  
Harmonic Generation ◽  
Cancer Progression ◽  
Cell Biology ◽  
Second Harmonic ◽  
Human Tumor ◽  
Matrix Remodeling ◽  
Tissue Samples

AbstractExtracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen is the most abundant protein, making up to 90% of ECM, and 30% of total protein weight in humans. Second-harmonic generation (SHG) microscopy represents an important tool to study collagen organization of ECM in freshly unfixed tissues and paraffin-embedded tissue samples. This manuscript aims to review some of the applications of SHG microscopy in Oncologic Pathology, mainly in the study of ECM of epithelial tumors. It is shown how collagen parameters measured by this technique can aid in the differential diagnosis and in prognostic stratification. There is a tendency to associate higher amount, lower organization and higher linearity of collagen fibers with tumor progression and metastasizing. These represent complex processes, in which matrix remodeling plays a central role, together with cancer cell genetic modifications. Integration of studies on cancer cell biology and ECM are highly advantageous to give us a more complete picture of these processes. As microscopic techniques provide topographic information allied with biologic characteristics of tissue components, they represent important tools for a more complete understanding of cancer progression. In this context, SHG has provided significant insights in human tumor specimens, readily available for Pathologists.

Sign in / Sign up

Export Citation Format

Share Document