scholarly journals Gc-Ms Analysis and Antibacterial Activity of Mangosteen Leaf Extracts against Plant Pathogenic Bacteria

2016 ◽  
Vol 07 (07) ◽  
pp. 1013-1020 ◽  
Author(s):  
Qamar Mohammed Naji Alsultan ◽  
Kamaruzaman Sijam ◽  
Tavga Sulaiman Rashid ◽  
Khairulmazmi Bin Ahmad
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Leaf Extracts ◽  
Plant Pathogenic Bacteria ◽  
Ms Analysis

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals In vivo and in vitro antibacterial activity of neomycin against plant pathogenic bacteria

2011 ◽  
Vol 6 (34) ◽  
pp. 6829-6834, ◽  
Author(s):  
Tao Ke ◽  
Fan Jieyu ◽  
Shi Guanying ◽  
Zhang Xingang ◽  
Zhao Haoyu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Plant Pathogenic Bacteria ◽  
In Vitro Antibacterial Activity

scholarly journals Antibacterial Activities of Xanthium Strumarium L.

2018 ◽  
Vol 29 ◽  
pp. 70-77 ◽  
Author(s):  
Anjana Devkota ◽  
Ritu Kumari Das
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Xanthium Strumarium ◽  
Distilled Water ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. The antibacterial activity was studied against six pathogenic bacteria, three gram negative: Klebsiella pneumoniae (ATCC 15380), Proteus mirabilis (ATCC 49132), Escherichia coli (ATCC 25922) and three gram positive: Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25932) at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ ml, 250 mg/ml) of leaf extracts of X. strumurium. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antibacterial activity of extracts was determined by disc diffusion method and zone of inhibition (ZOI) was measured. Gram negative bacteria was found more resistant than gram positive bacteria. The most susceptible bacterium was S. aureus while the most resistant bacterium was E. coli. Methanolic extract was found more effective than distilled water. These findings suggest that extracts obtained from leaves of X. strumurium possess biobactericidal potential, which can suitably be exploited for making antibacterial drugs.J. Nat. Hist. Mus. Vol. 29, 2015, Page: 70-77

Antibacterial activity of caffeine against plant pathogenic bacteria

2015 ◽  
Vol 62 (3) ◽  
pp. 605-612 ◽  
Author(s):  
Wojciech Sledz ◽  
Emilia Los ◽  
Agnieszka Paczek ◽  
Jacek Rischka ◽  
Agata Motyka ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Plant Pathogenic Bacteria

scholarly journals Evaluation of the Phytochemical and Antibacterial characteristics of leaf extracts of Xanthium strumarium L. against Bacteria

2020 ◽  
Vol 11 (1) ◽  
pp. 725-729
Author(s):  
Antu Kurrey ◽  
Lata Sharma ◽  
Shishir Tiwari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Herbal Medicines ◽  
Wild Plant ◽  
Xanthium Strumarium ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Maximum Inhibition

Plants are one of the large sources of herbal medicines. Many plants have the capability to produce some bioactive constituents which give defense against microorganisms like bacteria and fungi and also from insects. Xanthium strumarium L. is a wild plant that grows like a weed. Leaves of  Xanthium strumarium L. were subjected for extraction in 4 different solvents viz. methanol, chloroform, aqueous, and ethanol. The aim of this study was to investigate the phytochemical screening and antimicrobial activity of methanol, chloroform, aqueous, and ethanol extracts prepared from leaves of  Xanthium strumarium L. The antibacterial activity was assessed by using the agar well diffusion assay against tested bacterial strain, Escherichia coli (CGSC 4312) and Bacillus subtilis (ATCC 9634). Phytochemical analysis has revealed that  Xanthium strumarium L. has most of the significant phytoconstituents like Saponins, Terpenoids, Flavanoids, Phenol, steroids, Anthraquinones, Tannins,  Alkaloids,  Glycosides, and Carbohydrate. The zone of inhibition was measured and compared by standard antibiotic streptomycin. The outcome of the present work showed that chloroform extract (400µg/ml) showed maximum inhibition against E. Coli (23mm), and Ethanol extract(400µg/ml) showed maximum inhibition against Bacillus subtilis (24mm). The result divulged the presence of antibacterial activity of different extracts of Xanthium strumarium L.against human pathogenic bacteria. Thus, this plant has many active compounds that can be used for the development of various potent drugs.

scholarly journals Composition and Antibacterial Activity of Aronia melanocarpa (Michx.) Elliot, Cornus mas L. and Chaenomeles superba Lindl. Leaf Extracts

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2011 ◽  
Author(s):  
Magdalena Efenberger-Szmechtyk ◽  
Agnieszka Nowak ◽  
Agata Czyżowska ◽  
Alicja Z. Kucharska ◽  
Izabela Fecka
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Hydroxycinnamic Acid ◽  
Total Phenolic ◽  
Lag Phase ◽  
Leaf Extracts ◽  
Acinetobacter Baumanii ◽  
Aronia Melanocarpa ◽  
Cornus Mas ◽  
Hydroxycinnamic Acid Derivatives

The purpose of this study was to investigate the composition of leaf extracts from Aronia melanocarpa, Chaenomeles superba, and Cornus mas, and their antimicrobial activity against typical spoilage-causing and pathogenic bacteria found in meat and meat products. The highest total phenolic content (TPC) was detected in C. superba extract, followed by C. mas and A. melanocarpa extracts. The antioxidant capacity of the extracts was measured by DPPH and ABTS assays. The lowest IC50 values were found for C. superba extract, followed by C. mas and A. melanocarpa extracts. LC-MS and HPLC analysis revealed that A. melanocarpa and C. superba extracts contained hydroxycinnamic acid derivatives and flavonoids (mainly flavonols). Hydroxycinnamic acid derivatives were detected in the C. mas extract, as well as flavonols, ellagitannins, and iridoids. The antibacterial activity of the plant extracts was tested against Gram-negative bacteria (Moraxella osloensis, Pseudomonas fragi, Acinetobacter baumanii, Escherichia coli, Enterobacter aerogenes, Salmonella enterica) and Gram-positive bacteria (Enterococcus faecium, Staphylococcus aureus, Brochothrix thermosphacta, Lactobacillus sakei, Listeria monocytogenes) using the microculture method. The extracts acted as bacteriostatic agents, decreasing the growth rate (µmax) and extending the lag phase (tlag). C. mas showed most potent antibacterial activity, as confirmed by principal component analysis (PCA).

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