scholarly journals Genetic Diversity of <i>Fusarium solani</i> f. sp. <i>cucurbitae</i>, the Causal Root and Crown Rot of Cucurbits (Melon) by Using Molecular Markers and Control

2016 ◽  
Vol 07 (15) ◽  
pp. 2151-2172 ◽  
Author(s):  
Falah Abdul-Hasan ◽  
Halima Z. Hussein
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Fusarium Solani ◽  
Crown Rot ◽  
Root And Crown Rot ◽  
And Control

scholarly journals Retrotransposon-Based Molecular Markers for Analysis of Genetic Diversity within the GenusLinum

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Nataliya V. Melnikova ◽  
Anna V. Kudryavtseva ◽  
Alexander V. Zelenin ◽  
Valentina A. Lakunina ◽  
Olga Yu. Yurkevich ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Taxonomic Revision ◽  
Chromosome Localization ◽  
And Control ◽  
Genebank Collections

SSAP method was used to study the genetic diversity of 22Linumspecies from sectionsLinum,Adenolinum, Dasylinum, Stellerolinum, and 46 flax cultivars. All the studied flax varieties were distinguished using SSAP for retrotransposonsFL9andFL11. Thus, the validity of SSAP method was demonstrated for flax marking, identification of accessions in genebank collections, and control during propagation of flax varieties. Polymorphism ofFl1a, Fl1b, andCassandrainsertions were very low in flax varieties, but these retrotransposons were successfully used for the investigation ofLinumspecies. Species clusterization based on SSAP markers was in concordance with their taxonomic division into sectionsDasylinum, Stellerolinum, Adenolinum, andLinum. All species of sect.Adenolinumclustered apart from species of sect.Linum. The data confirmed the accuracy of the separation in these sections. Members of sectionLinumare not as closely related as members of other sections, so taxonomic revision of this section is desirable.L. usitatissimumaccessions genetically distant from modern flax cultivars were revealed in our work. These accessions are of utmost interest for flax breeding and introduction of new useful traits into flax cultivars. The chromosome localization ofCassandraretrotransposon inLinumspecies was determined.

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

Assessment of genetic diversity of pumpkins (Cucurbita spp.) from western Kenya using SSR molecular markers

2021 ◽  
Vol 48 (3) ◽  
pp. 2253-2260
Author(s):  
Lameck A. Nyabera ◽  
Inosters W. Nzuki ◽  
Steven M. Runo ◽  
Peris W. Amwayi
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Western Kenya

scholarly journals Effect of Ionizing Radiation on the Bacterial and Fungal Endophytes of the Halophytic Plant Kalidium schrenkianum

2021 ◽  
Vol 9 (5) ◽  
pp. 1050
Author(s):  
Jing Zhu ◽  
Xiang Sun ◽  
Zhi-Dong Zhang ◽  
Qi-Yong Tang ◽  
Mei-Ying Gu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ionizing Radiation ◽  
Endophytic Bacteria ◽  
Fungal Endophytes ◽  
Root Endophytes ◽  
Fungal Community Structure ◽  
Geochemical Factors ◽  
Bacteria And Fungi ◽  
High Level ◽  
And Control

Endophytic bacteria and fungi colonize plants that grow in various types of terrestrial and aquatic ecosystems. Our study investigates the communities of endophytic bacteria and fungi of halophyte Kalidium schrenkianum growing in stressed habitats with ionizing radiation. The geochemical factors and radiation (at low, medium, high level and control) both affected the structure of endophytic communities. The bacterial class Actinobacteria and the fungal class Dothideomycetes predominated the endophytic communities of K. schrenkianum. Aerial tissues of K. schrenkianum had higher fungal diversity, while roots had higher bacterial diversity. Radiation had no significant effect on the abundance of bacterial classes. Soil pH, total nitrogen, and organic matter showed significant effects on the diversity of root endophytes. Radiation affected bacterial and fungal community structure in roots but not in aerial tissues, and had a strong effect on fungal co-occurrence networks. Overall, the genetic diversity of both endophytic bacteria and fungi was higher in radioactive environments, however negative correlations were found between endophytic bacteria and fungi in the plant. The genetic diversity of both endophytic bacteria and fungi was higher in radioactive environments. Our findings suggest that radiation affects root endophytes, and that the endophytes associated with aerial tissues and roots of K. schrenkianum follow different mechanisms for community assembly and different paradigms in stress response.

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