scholarly journals Anthelminthic Activity of <i>Moringa oleifera</i> Leaf Extracts Evaluated <i>in Vitro</i> on Four Developmental Stages of <i>Haemonchus contortus</i> from Goats

2014 ◽  
Vol 05 (11) ◽  
pp. 1702-1710 ◽  
Author(s):  
Gertrude Mbogning Tayo ◽  
Josué Wabo Poné ◽  
Marie Claire Komtangi ◽  
Jeannette Yondo ◽  
Alidou Marc Ngangout ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Moringa Oleifera ◽  
Developmental Stages ◽  
Leaf Extracts ◽  
Anthelminthic Activity

scholarly journals Efficacy of monepantel against lower developmental stages of a multi-resistant and susceptible Haemonchus contortus isolates: an in vitro study

2013 ◽  
Vol 50 (2) ◽  
pp. 91-95 ◽  
Author(s):  
L. Lecová ◽  
L. Stuchlíková ◽  
J. Lamka ◽  
M. Špulák ◽  
M. Várady ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Developmental Stages ◽  
In Vitro Study ◽  
Lethal Concentration ◽  
In Vitro Tests ◽  
High Efficacy ◽  
Larval Stages ◽  
New Class ◽  
Ovicidal Effect

AbstractMonepantel (MOP) belongs to a new class of anthelmintic compounds, the amino-acetonitrile derivates, which have a different mode of action as the currently used anthelmintics. Many present studies confirmed the high efficacy of MOP against fourth larval and adult stages of Haemonchus contortus. The objective of this study was to determine in vitro efficacy of MOP against lower development stages (eggs, L1–L3 larvae) and to compare it between resistant and susceptible isolates of H. contortus. For this purpose, two in vitro tests - egg hatch test and micro-agar larval development test were used. Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99) and resistance factor (RF). This study revealed the high efficacy against lower larval stages (L1–L3) of both resistant and susceptible strains of this parasite. Larval susceptibility was not dependent of the sensitivity status of the nematode isolate. On the other hand, ovicidal effect of MOP was very low.

scholarly journals Effectiveness Comparison Between Young Leaf Extracts Acacia nilotica with Desmanthus virgatus Against Vermicidal Potency of Haemonchus contortus In-vitro

2021 ◽  
Vol 39 (2) ◽  
pp. 168
Author(s):  
I Gusti Komang Oka Wirawan ◽  
Aholiab Aoetpah ◽  
Jois Moriani Jacob
Keyword(s):  
Haemonchus Contortus ◽  
Immersion Time ◽  
Positive Control ◽  
Negative Control ◽  
Acacia Nilotica ◽  
Leaf Extracts ◽  
Tropical Countries ◽  
Young Leaves ◽  
Mortality Percentage

Acacia nillotica and Desmanthus virgatus are two entopharmacologycal plants that thrives throughout the season in East Nusa Tenggara Province, other Provinces in Indonesia or tropical countries. Extraction of young leaves of Acacia nillotica (EDMAN) and Desmanthus virgatus (EDMDV) contains tannin compound. By pharmacodynamic viewpoint, this extraction has potency as an anthelmintic. Objective: to compare the in-vitro effectivity of young leaves extraction of the two plants as a vermicidal power to combat Haemonchus contortus. Materials: young leaves of Acacia nillotica and Desmanthus virgatus and Haemonchus contortus. Method: The study was grouped into four treatments: EDMAN, EDMDV, Positive control (Albendazole 0,055%) and negative control (aquades).  The concentration of the young leaves extracts are 2.5%, 3.5%, 4.5% out of 0.01 g/mL of extraction. Each treatment was applied to 6 female Haemonchus contortus with four replicates allowing immersion time for 1, 3, 5 or 7 hours. Variable measured and tested was mortality of the H. contortus. The vermicidal effectively was descriptively analysed. The results showed that mortality percentage (vermicidal) treatment of 2.5%, 3.5%, 4.5% EDMAN for 7-hour immersion was 16.7%, 45.8%, 12.5%, respectively. That values for EDMDV for similar concentrations and immersion time was 50%, 33.3%, 12.5%, respectively. Conclusion: EDMDV has a more effective vermicidal power between the two etnopharmacological treatments at 2.5% concentration.

scholarly journals In Vitro and In Silico Screening and Characterization of Antimicrobial Napin Bioactive Protein in Brassica juncea and Moringa oleifera

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2080
Author(s):  
Sangeeta Chandrashekar ◽  
Raman Vijayakumar ◽  
Ramachandran Chelliah ◽  
Eric Banan-Mwine Daliri ◽  
Inamul Hasan Madar ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Brassica Juncea ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Cell Aggregation ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Inhibition Concentration ◽  
Potent Inhibition

The study aimed to investigate the antibacterial activity of Mustard (Brassica juncea) and Moringa (Moringa oleifera) leaf extracts and coagulant protein for their potential application in water treatment. Bacterial cell aggregation and growth kinetics studies were employed for thirteen bacterial strains with different concentrations of leaf extracts and coagulant protein. Moringa oleifera leaf extract (MOS) and coagulant protein showed cell aggregation against ten bacterial strains, whereas leaf extract alone showed growth inhibition of five bacterial strains for up to 6 h and five bacterial strains for up to 3 h. Brassica juncea leaf extract (BJS) showed growth inhibition for up to 6 h, and three bacterial strains showed inhibition for up to 3 h. The highest inhibition concentration with 2.5 mg/mL was 19 mm, and furthermore, the minimum inhibitory concentration (MIC) (0.5 mg/mL) and MBC (1.5 mg/mL) were determined to have a higher antibacterial effect for <3 KDa peptides. Based on LCMS analysis, napin was identified in both MOS and BJS; furthermore, the mode of action of napin peptide was determined on lipoprotein X complex (LpxC) and four-chained structured binding protein of bacterial type II topoisomerase (4PLB). The docking analysis has exhibited moderate to potent inhibition with a range of dock score −912.9 Kcal/mol. Thus, it possesses antibacterial-coagulant potential bioactive peptides present in the Moringa oleifera purified protein (MOP) and Brassica juncea purified protein (BJP) that could act as an effective antimicrobial agent to replace currently available antibiotics. The result implies that MOP and Brassica juncea purified coagulant (BJP) proteins may perform a wide degree of antibacterial functions against different pathogens.

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