scholarly journals Genetic Distance Estimated by RAPD Markers and Performance of Topcross Hybrids in Popcorn

2012 ◽  
Vol 03 (12) ◽  
pp. 1666-1673
Author(s):  
Silvia Graciele Hülse de Souza ◽  
Valéria Carpentieri-Pípolo ◽  
Deoclécio Domingos Garbúglio ◽  
Nelson da Silva Fonseca Júnior ◽  
Claudete de Fátima Ruas ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
And Performance ◽  
Topcross Hybrids

Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes

2011 ◽  
Vol 343-344 ◽  
pp. 981-987
Author(s):  
Feng Juan Li ◽  
Chang Lu Wang ◽  
Dong He ◽  
Ya Qiong Liu ◽  
Mian Hua Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Oil Content ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Ricinus Communis ◽  
Major Group ◽  
Ricinus Communis L ◽  
Polymorphic Bands

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.

Molecular Diversity Analysis of Lentil (Lens culinaris Medik.) through RAPD Markers

2012 ◽  
Vol 22 (1) ◽  
pp. 51-58 ◽  
Author(s):  
M.E. Hoque ◽  
M.M. Hasan
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Group Method ◽  
Diversity Analysis ◽  
Pair Group ◽  
Molecular Genetic Diversity

Random Amplified Polymorphic DNA (RAPD) markers were used to study the molecular genetic diversity analysis among six BARI released lentil varieties viz. BARI masur-1, BARI masur-2, BARI masur-3, BARI masur-4, BARI masur-5 and BARI masur-6. PCR amplified products were visualized on 1.0% agarose gel and the band for each primer were scored. Ten RAPD markers were used in this study. Out of them 7 primers showed amplification of 53 DNA fragments with 60.37% of them being polymorphic. The highest number of polymorphic loci was noticed in the variety BARI masur-3. The same variety also showed maximum Nei’s gene diversity value (0.0552). The highest Nei’s genetic distance (0.5002) was observed in BARI masur-1 vs. BARI masur-5 whereas, the lowest genetic distance (0.0692) was found in BARI masur-1 vs. BARI masur-2. The unweighted pair group method of arithmetic mean (UPGMA) dendrogram based on Nei’s genetic distance grouped the six cultivars into two main clusters. BARI masur-1, BARI masur-2 and BARI masur-3 were in cluster I and BARI masur-4, BARI masur-5 and BARI masur-6 were in cluster II. The cultivar BARI masur-4 was closest to the cultivar BARI masur-6 with the lowest genetic distance (0.0972) and the highest genetic distance (0.5002) was found between BARI masur-1 and BARI masur-5. The RAPD markers were found to be useful in molecular characterization of lentil varieties which could be utilized by the breeders for the improvement of lentil cultivars. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11260 Plant Tissue Cult. & Biotech. 22(1): 51-58, 2012 (June)

Rapd variation of late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces from Ethiopia

2007 ◽  
Vol 55 (3) ◽  
pp. 375-382 ◽  
Author(s):  
S. Mamo ◽  
A. Ayana ◽  
T. Tesso
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Sorghum Bicolor ◽  
Rapd Markers ◽  
Diversity Index ◽  
Random Amplified Polymorphic Dna ◽  
The Mean ◽  
Polymorphic Bands ◽  
High Degree ◽  
Sorghum Bicolor L

A study on the extent and pattern of genetic variability in late-maturing sorghum [ Sorghum bicolor (L.) Moench] landraces collected from the Wello and Hararge areas of Ethiopia was conducted using random amplified polymorphic DNA (RAPD) markers for 70 individuals representing 14 populations. Four oligonucleotide primers generated a total of 55 polymorphic bands with 13–19 bands per primer and a mean of 16 bands across the 70 individuals. The value of the Shannon diversity index among the populations (0.26) and between the two regions (0.24) was low to moderate, despite the high degree of polymorphic bands per primer. The mean genetic distance (0.25) between the populations was found to be low. The low genetic variation may be due to the reduced population size of late-maturing sorghum landraces in the two regions of Ethiopia because of farmers’ decisions in the process of planting, managing, harvesting and processing their crops. Partitioning of the genetic variation into variation between and within the population revealed that 92.9% and 7.10% of the variation was found to be between and within the populations, respectively. Cluster analysis of genetic distance estimates further confirmed a low level of differentiation in late-maturing sorghum populations both between and within the regions. The implications of the results for genetic conservation purposes are discussed.

Use of RAPD analyses to estimate population genetic parameters in the alfalfa leaf-cutting bee, Megachile rotundata

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 655-663 ◽  
Author(s):  
Rui Lu ◽  
Gerald H. Rank
Keyword(s):  
Genetic Distance ◽  
Population Genetic ◽  
Rapd Markers ◽  
Genetic Parameters ◽  
Megachile Rotundata ◽  
Isolated Populations ◽  
Leaf Cutting ◽  
Nucleotide Divergence ◽  
Alfalfa Leaf ◽  
Population Genetic Parameters

RAPD analyses were performed on five geographically isolated populations of Megachile rotundata. We used haploid males of the alfalfa leaf-cutting bee, M. rotundata, to overcome the limitation of the dominance of RAPD markers in the determination of population genetic parameters. Sixteen primers gave rise to 130 polymorphic and 31 monomorphic bands. The unbiased estimators calculated in this study include within- and between-population heterozygosity, nucleotide divergence, and genetic distance. The genetic diversity (H = 0.32–0.35) was found to be about 10 times that of previous estimates (H = 0.033) based on allozyme data. Contrary to the data obtained at the protein level, our results suggest that Hymenoptera do not have a lower level of genetic variability at the DNA level compared with other insect species. Regardless of the different assumptions underlying the calculation of heterozygosity, divergence, and genetic distance, all five populations showed a parallel interrelationship for the three parameters. We conclude that RAPD markers are a convenient tool to estimate population genetic variation in haploid M. rotundata and that with an adequate sample size the technique is applicable to the evaluation of divergence in diploid populations. Key words : Megachile rotundata, RAPD, heterozygosity, genetic distance, nucleotide divergence.

scholarly journals Molecular Analysis of Hexaploid Wheat (Triticum aestivum L.) Cultivars by RAPD Markers

1970 ◽  
Vol 19 (1) ◽  
pp. 35-44 ◽  
Author(s):  
S. Mitra ◽  
K. M. Nasiruddin ◽  
E. H. Chowdhury
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Molecular Genetic Analysis ◽  
Wheat Cultivars ◽  
Polymorphic Loci ◽  
Upgma Dendrogram ◽  
Nei's Genetic Distance

RAPD assay was conducted for molecular genetic analysis of six wheat cultivars, such as, Kanchan, Sourav, Gourab, Shatabdi, Pavon and BAW-1006 to observe genetic variability and relatedness among these cultivars. Three out of 12 decamer random primers showed distinctly polymorphic bands when used to amplify genomic DNA. The primers yielded a total of 23 RAPD markers of which 14 were considered as polymorphic. The proportion of polymorphic loci and gene diversity (h) values were 34.78% and 0.153 for BAW-1006, 30.43% and 0.124 for Kanchan, 26.09% and 0.127 for Shatabdi, 26.09% and 0.127 for Pavon, 26.09% and 0.111 for Gourab, 21.74% and 0.098 for Sourav, respectively. The coefficient of gene differentiation (Gst) and gene flow (Nm) values across all the loci were 0.50 and 0.50, respectively indicating genetic divergence among populations. The UPGMA  dendrogram  based  on Nei’s  genetic distance, grouped  six cultivars into two main clusters:  Kanchan, Sourav, Gourab  and Shatabdi  in cluster I; Pavon and BAW-1006 in cluster II. The cluster I was further separated: Kanchan alone in sub-cluster I and Sourav, Gourab, Shatabdi in sub-cluster II; furthermore, Sourab and Gourab grouped together in sub-sub-cluster I of sub-cluster II with the lowest genetic distance of 0.035. Thus, RAPD offer a potentially simple, rapid and reliable method to evaluate genetic variation and relatedness among six wheat cultivars.  Key words: RAPD, genetic diversity, polymorphic loci, wheat D.O.I. 10.3329/ptcb.v19i1.4915 Plant Tissue Cult. & Biotech. 19(1): 35-44, 2009 (June)

scholarly journals Genetic Relationship Among Ten Promising Eggplant Varieties Using RAPD Markers

1970 ◽  
Vol 19 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Md. Sanaullah Biswas ◽  
Md. Abdullah Yousuf Akhond ◽  
Md. Al-Amin ◽  
Mahmuda Khatun ◽  
Muhammed Rezwan Kabir
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Relationship ◽  
Plant Tissue ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Similarity Index ◽  
The Other ◽  
Upgma Dendrogram ◽  
Potential Tool

RAPD technique was used as a tool for assessing genetic diversity and varietal relationships among ten varieties of eggplant. Out of 21 primers screened four were selected. With these primers 76 clear and bright fragments were obtained of which 44 fragments considered polymorphic. The proportion of polymorphic loci and gene diversity values across all loci were 57.89% and 0.23, respectively. The UPGMA dendrogram based on genetic distance segregated the ten varieties of eggplant into two main clusters. Dohazari, Kazla, Nayantara and ISD-006 were grouped together in cluster I whereas Uttara, Islampuri, Khatkhatia, Singnath, BARI Begun-08 and Eggplant Line-083 into cluster II. Kazla and Nayantara variety pair was very close to each other with the highest intervarietal similarity index (92.54%) and lowest genetic distance (0.14). On the other hand, Khatkhatia and Nayantara pair was the lowest intervarietal similarity index (41.67%) with highest genetic distance (0.48). Therefore, identification of genetically distinct varieties using RAPD markers could be a potential tool for eggplant improvement. Key words: Eggplant, Polymorphism, Genetic relationship, RAPD D.O.I. 10.3329/ptcb.v19i2.5006 Plant Tissue Cult. & Biotech. 19(2): 119-126, 2009 (December)

scholarly journals GENETIC VARIATION AND RELATEDNESS AMONG HIGH YIELDING RICE VARIETIES (Oryza sativa L.) REVEALED BY RAPD MARKERS

2008 ◽  
Vol 21 (1) ◽  
pp. 07-14
Author(s):  
F. Easmin ◽  
M. S. Rahman ◽  
M. S. Islam ◽  
M. A. Samad ◽  
M. S. Alam
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Oryza Sativa L ◽  
Gene Diversity ◽  
Chain Reaction ◽  
Rice Varieties ◽  
Upgma Dendrogram ◽  
Polymerase Chain ◽  
High Level

Genetic variation is a principal concern for the plant breeders. Genetic variation and relationship among high yielding rice varieties viz. Binadhan 4, Binadhan 5, Binadhan 6, Binasail, BRRI dhan28 and BRRI dhan29 were analyzed using four decamer random primers. Polymerase Chain Reaction (PCR) amplified 22 RAPD markers, of which 18 (81.82%) were polymorphic. The proportion of polymorphic loci and the gene diversity values were 59.09% and 0.25 for the Binadhan 4; 59.09% and 0.21 for Binadhan 6; 54.55% and 0.23 for Binasail; 54.55% and 0.19 for BRRI dhan29; 50.00% and 0.19 for Binadhan 5 and 45.45% and 0.18 for BRRI dhan28, respectively. The coefficient of gene differentiation (Gst) across all loci was calculated as 0.35 reflecting the existence of high level of genetic variation among the six modern rice varieties. UPGMA dendrogram based on Nei’s genetic distance segregated the six high yielding rice varieties into two clusters: all four mutant varieties viz. Binadhan 4, Binadhan 5, Binadhan 6 and Binasail formed one cluster and two varieties of BRRI grown in boro season, BRRI dhan28 and BRRI dhan29 grouped together in another cluster. Among the mutants, two boro season varieties, developed from the same parent, Binadhan 5 and Binadhan 6 grouped together with genetic distance of 0.10. Therefore, RAPD offer a reliable method to evaluate genetic variation and relatedness among the high yielding rice varieties.DOI: http://dx.doi.org/10.3329/bjpbg.v21i1.17042

scholarly journals Genetic diversity analysis in cashew (Anacardium occidentale L.) germplasm using RAPD marker

2019 ◽  
Vol 17 (4) ◽  
pp. 461-465
Author(s):  
T Bhadra ◽  
AZM Obaidullah ◽  
Mst Sabiha Sultana ◽  
M Ahmed ◽  
MM Islam
Keyword(s):  
Genetic Distance ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Climatic Conditions ◽  
Anacardium Occidentale ◽  
Cashew Nut ◽  
Major Cluster ◽  
The Family ◽  
Wide Potential

Anacardium occidentale L., commonly known as cashew nut, belongs to the family Anacardiaceae.  It is regarded as a high valued fruit nut crop world-wide. Potential of this economically important nut  is under-utilized in Bangladesh in spite of having all favorable agro-climatic conditions. The objective of the present investigation was to characterize six cashew accessions using Random Amplified Polymorphic DNA (RAPD) markers. Four random primers viz. OPE-02, OPE-18, OPK-03 and OPB-15 were used to amplify DNA segments. A total of 33 reproducible bands were obtained, out of which 11 were monomorphic and 22 were polymorphic. On average 74.12% polymorphism was observed. . Primers OPB-15 and OPK-03 yielded 100% polymorphism and OPE 02 and OPE 18 produced 33.33% and 63.16 % polymorphism, respectively. Cluster analysis revealed two main distinct groups, first group included GP-1 and the second consisted of five genotypes viz. GP-2, GP-3, GP-4, GP-5and GP-6 The major cluster- II was further subdivided into two minor clusters i.e. minor cluster- III and IV. Minor cluster- III contained only one genotype GP-4. Minor cluster- IV consists of four rest genotypes. The genetic distance between the groups was found low and varied from 0.002 to 0.0308. Maximum genetic distance was observed between GP-1 and GP-2cashew germplasm and minimum between GP-5 and GP-6. The low genetic distance which is unusual for this out crossing long-lived tree species, indicates the probability of having common ancestry among the germplasm or may be due to the use of a narrow range of populations for the investigation. J Bangladesh Agril Univ 17(4): 461–465, 2019

scholarly journals Genetic diversity analysis in Brassica varieties through RAPD markers

1970 ◽  
Vol 34 (3) ◽  
pp. 493-503 ◽  
Author(s):  
KK Ghosh ◽  
ME Haque ◽  
S Parvin ◽  
F Akhter ◽  
MM Rahim
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Arithmetic Mean ◽  
Polymorphic Band ◽  
Group Method ◽  
Polymorphic Loci ◽  
Pair Group

This investigation was aimed at exploring the genetic diversity and relationship among nine Brassica varieties, namely BARI Sharisha-12, Agrani, Sampad, BINA Sharisha-4, BINA Sharisha-5, BARI Sharisha-13, Daulot, Rai-5, Alboglabra using Random Amplified Polymorphic DNA (RAPD) markers. In total, 59 reproducible DNA bands were generated by four arbitrary selected primers of which 58 (98.03%) bands were proved to be polymorphic. These bands ranged from 212 to 30686 bp in size. The highest proportion of polymorphic loci and gene diversity values were 37.29% and 0.1373, respectively, for BARI Sharisha-12 and the lowest proportion of polymorphic loci and gene diversity values were 8.47% and 0.0318, 8.47% and 0.0382 for BINA Sharisha-4 and Rai-5, respectively. A dendrogram was constructed using unweighted pair group method of arithmetic mean (UPGMA). The result of cluster analysis indicated that the 9 accessions were capable of being classified into 2 major groups. One group consists of BARI Sharisha-12, Agrani, Sampad, Daulot, Rai-5, Alboglabra. where Daulot and Rai-5 showed the lowest genetic distance of 0.049. And another group contains BINA Sharisha-4, BINA Sharisha-5, and BARI Sharisha-1 3, where BINA Sharisha-5 and BARI sharisha-13 showed genetic distance of 0.071. Key Words: RAPD, Brassica, genetic distance, polymorphic band. DOI: 10.3329/bjar.v34i3.3976 Bangladesh J. Agril. Res. 34(3) : 493-5032, September 2009

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