scholarly journals Comparative Analysis of Protein Expression Concomitant with DNA Methyltransferase 3A Depletion in a Melanoma Cell Line

2011 ◽  
Vol 02 (05) ◽  
pp. 539-572 ◽  
Author(s):  
Xiaoyan Liu ◽  
Shengnan Tang ◽  
Tonghua Li ◽  
Haoyue Wang ◽  
Jiangming Sun ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Protein Expression ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Dna Methyltransferase ◽  
Dna Methyltransferase 3A

scholarly journals Protein expression changes induced in a malignant melanoma cell line by the curcumin analogue compound D6

BMC Cancer ◽  
2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Marina Pisano ◽  
Antonio Palomba ◽  
Alessandro Tanca ◽  
Daniela Pagnozzi ◽  
Sergio Uzzau ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Protein Expression ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Malignant Melanoma Cell ◽  
Curcumin Analogue

scholarly journals UROKINASE AND ITS RECEPTOR IN CUTANEOUS MELANOMA REPRODUCED IN CHRONIC NEUROGENIC PAIN IN MICE OF BOTH GENDERS IN COMPARISON

2020 ◽  
Vol 66 (4) ◽  
pp. 445-450
Author(s):  
Oleg Kit ◽  
Yelena Frantsiyants ◽  
Larisa Kozlova ◽  
Irina Kaplieva ◽  
Valeriya Bandovkina ◽  
...  
Keyword(s):  
Gender Differences ◽  
Comparative Analysis ◽  
Cell Line ◽  
Melanoma Cell ◽  
Cutaneous Melanoma ◽  
Melanoma Cell Line ◽  
Malignant Tumors ◽  
Intact Skin ◽  
Neurogenic Pain ◽  
Terminal Stage

Aim: comparative analysis of plasmin, plasminogen (PG), urokinase PG activator (uPA) and its receptor (uPAR) in tissues of malignant tumors and intact skin of С57ВЬ/6 mice of both genders in В16/F10 melanoma reproduced in chronic neurogenic pain (CNP). Methods: The study was performed on 130 mice and В16/ F10 melanoma cell line. Levels of uPA-antigen (uPA-AG) and uPA-activity (uPA-act), uPAR, PG, PAP (plasmin-a2-antiplasmin complex) were determined by ELISA. Statistics: the Statistica 10 program. Results. CNP-induced depletion of the content and activity of uPA was maintained in the skin and tumors after melanoma transplantation. Elevation of uPAR in females under the influence of CNP after the transplantation normalized at the terminal stage only; decreased levels of uPAR in males were maintained during the whole experiment. Elevation of PAP in females with CNP was maintained in the skin and tumors after the transplantation, and in males it was less marked. CNP affected the time of development of transplantable В16/F10 melanoma in female and male С57ВL/6 mice, changed gender differences typical of the standard tumor transplantation, decreased the period prior to the development of metastases, increased their number and extended localization. Conclusions. The study confirmed involvement of plasmin and uPAR in the formation of В16/F10 melanoma in CNP with changing gender priorities in development and spread of the process.

Predicting the possible effect of miR-203a-3p and miR-29a-3p on DNMT3B and GAS7 genes expression

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Afgar Ali ◽  
Sattarzadeh Bardsiri Mahla ◽  
Vahidi Reza ◽  
Farsinejad Alireza
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Dna Methyltransferase ◽  
Critical Role ◽  
Regulatory Effect ◽  
Aberrant Expression ◽  
Expression Of Genes ◽  
Genes Expression ◽  
A375 Cell Line

Abstract Aberrant expression of genes involved in methylation, including DNA methyltransferase 3 Beta (DNMT3B), can cause hypermethylation of various tumor suppressor genes. In this regard, various molecular factors such as microRNAs can play a critical role in regulating these methyltransferase enzymes and eventually downstream genes such as growth arrest specific 7 (GAS7). Accordingly, in the present study we aimed to predict regulatory effect of miRNAs on DNMT3B and GAS7 genes expression in melanoma cell line. hsa-miR-203a-3p and hsa-miR-29a-3p were predicted and selected using bioinformatics software. The Real-time PCR technique was performed to investigate the regulatory effect of these molecules on the DNMT3B and GAS7 genes expression. Expression analysis of DNMT3B gene in A375 cell line showed that there was a significant increase compared to control (p value = 0.0015). Analysis of hsa-miR-203a-3p and hsa-miR-29a-3p indicated the insignificant decreased expression in melanoma cell line compared to control (p value < 0.05). Compared to control, the expression of GAS7 gene in melanoma cells showed a significant decrease (p value = 0.0323). Finally, our findings showed that the decreased expression of hsa-miR-203a-3p and hsa-miR-29a-3p can hypothesize that their aberrant expression caused DNMT3B dysfunction, possible methylation of the GAS7 gene, and ultimately decreased its expression. However, complementary studies are necessary to definite comment.

Insight into Mechanistic Action of Thymoquinone Induced Melanogenesis in Cultured Melanocytes

2019 ◽  
Vol 26 (12) ◽  
pp. 910-918
Author(s):  
Kamal U. Zaidi ◽  
Firoz N. Khan ◽  
Sharique A. Ali ◽  
Kausar P. Khan
Keyword(s):  
Western Blot ◽  
Signaling Pathways ◽  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Protein Kinase Inhibitors ◽  
Tyrosinase Activity ◽  
Dependent Manner ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cell

Background: Melanin plays a crucial role in camouflage, social communication and protection against harmful ultraviolet radiations. Melanin is synthesized by melanocytes through melanogenesis and several intrinsic and extrinsic factors are involved during the process. Any change occuring in the normal melanogenesis process can cause severe pigmentation problems of hypopigmentation or hyperpigmentation. Objective: The present study is based on the evaluation of the effect of thymoquinone on melanogenesis and their possible mechanism of action using the B16F10 melanoma cell line for the production via blocking signaling pathways. Methods: Phase contrast microscopy, cell viability, tyrosinase activity, melanin content and western blot analysis were used in the present study. Results: In the present investigation, cultured melanocytes exhibit that the stimulation of melanin synthesis when treated with thymoquinone. Tyrosinase activity and melanin production in B16F10 melanoma cell line was increased in doze-dependent manner. In western blot, we investigated the involvement of the cAMP/PKA pathway in thymoquinone induced melanogenesis. It was observed protein kinase inhibitors PKA, PKC, PKB and MEK1 decreased the stimulatory effects of thymoquinone from 11.45- fold value to 8.312, 6.631, 4.51, and 7.211-fold value, respectively. However, the results also prove that thymoquinone may partially induce tyrosinase expression via PKA, PKB, PKC and MEK1 signaling pathways. Conclusion: The present finding proposed that thymoquinone is a protective challenger for melanogenesis and it might be useful for the treatment of hypopigmentary disorders.

scholarly journals In Vitro Anticancer Potential of Jasione montana and Its Main Components Against Human Amelanotic Melanoma Cells

2021 ◽  
Vol 22 (7) ◽  
pp. 3345
Author(s):  
Aleksandra Maria Juszczak ◽  
Robert Czarnomysy ◽  
Jakub Władysław Strawa ◽  
Marijana Zovko Končić ◽  
Krzysztof Bielawski ◽  
...  
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Binding Assay ◽  
Annexin V ◽  
Melanoma Cells ◽  
Amelanotic Melanoma ◽  
Cytotoxic Activities ◽  
Ionization Mass ◽  
Caspase 9

Jasione montana L. (Campanulaceae) is used in traditional Belarusian herbal medicine for sleep disorders in children, but the chemical composition and biological activity have not been investigated. In this study, the activities of J. montana extracts, their fractions and main compounds were evaluated in amelanotic melanoma C32 (CRL-1585) cells and normal fibroblasts (PCS-201-012). The extracts and fractions were analyzed using liquid chromatography–photodiode array detection–electrospray ionization–mass spectrometry (LC–PDA–ESI–MS/TOF) to characterize 25 compounds. Further, three major and known constituents, luteolin (22) and its derivatives such as 7-O-glucoside (12) and 7-O-sambubioside (9) were isolated and identified. The cytotoxic activities against fibroblasts and the amelanotic melanoma cell line were determined using the fixable viability stain (FVS) assay. The influence of diethyl ether (Et2O) fraction (JM4) and 22 on apoptosis induction was investigated using an annexin V binding assay. The obtained results showed significant cytotoxicity of JM4 and 22 with IC50 values of 119.7 ± 3.2 and 95.1 ± 7.2 μg/mL, respectively. The proapoptotic potential after 22 treatment in the C32 human amelanotic melanoma cell line was comparable to that of vinblastine sulfate (VLB), detecting 29.2 ± 3.0% apoptotic cells. Moreover, 22 displayed less necrotic potential against melanoma cells than VLB. In addition, the influences of JM4 and 22 on the dysfunction of the mitochondrial membrane potential (MMP), cell cycle and activity of caspases 3, 8, 9, and 10 were established. The effects of JM4 on MMP change (74.5 ± 3.0% of the cells showed a reduced MMP) corresponded to the results obtained from the annexin V binding assay and activation of caspase-9. JM4 and 22 displayed a significant impact on caspase-9 (40.9 ± 2.4% of the cells contained active caspase-9 after JM4 treatment and 16.6 ± 0.8% after incubation with 22) and the intrinsic (mitochondrial) apoptotic pathway. Moreover, studies have shown that JM4 and 22 affect the activation of external apoptosis pathways by inducing the caspase-8 and caspase-10 cascades. Thus, activation of caspase-3 and DNA damage via external and internal apoptotic pathways were observed after treatment with JM4 and 22. The obtained results suggest that J. montana extracts could be developed as new topical preparations with potential anticancer properties due to their promising cytotoxic and proapoptotic potential.

scholarly journals Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation

2020 ◽  
Vol 11 ◽  
Author(s):  
Otto Luiz Dutra Cerqueira ◽  
Maria Alejandra Clavijo-Salomon ◽  
Elaine Cristina Cardoso ◽  
Tharcisio Citrangulo Tortelli Junior ◽  
Samir Andrade Mendonça ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Line ◽  
Melanoma Cell ◽  
Immune Activation ◽  
Melanoma Cell Line ◽  
Human Melanoma ◽  
Human Melanoma Cell Line ◽  
Human Melanoma Cell ◽  
Interferon Β

scholarly journals MIF inhibition reverts the gene expression profile of human melanoma cell line-induced MDSCs to normal monocytes

Genomics Data ◽  
2016 ◽  
Vol 7 ◽  
pp. 240-242 ◽  
Author(s):  
Sabine Waigel ◽  
Beatriz E. Rendon ◽  
Gwyneth Lamont ◽  
Jamaal Richie ◽  
Robert A. Mitchell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Human Melanoma ◽  
Human Melanoma Cell Line ◽  
Human Melanoma Cell

CELL KINETIC STUDIES OF THE EFFECT OF VARIOUS KINDS OF CHEMOTHERAPEUTIC AGENTS ON A HUMAN MELANOMA CELL LINE (SEKI, II)

1982 ◽  
Vol 9 (4) ◽  
pp. 271-278 ◽  
Author(s):  
Shinji Shimada ◽  
Makoto Kawashima ◽  
Shinichi Watanabe ◽  
Kiyoshi Yamada ◽  
Masako Mizoguchi ◽  
...  
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Kinetic Studies ◽  
Chemotherapeutic Agents ◽  
Human Melanoma ◽  
Human Melanoma Cell Line ◽  
Human Melanoma Cell ◽  
Cell Kinetic
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